今週の輪読では下記の論文を取り上げました．
516. Macho, A.P. et al. (2014). A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science 343: 1509–1512.
517. Qiu, F. et al. (2014). 2D NMR barcoding and differential analysis of complex mixtures for chemical identification: the Actaea triterpenes. Anal. Chem. 86: 3964–3972.
518. Hoi, K.K. et al. (2014). Dissecting the insect metabolic machinery using twin ion mass spectrometry: a single P450 enzyme metabolizing the insecticide imidacloprid in vivo. Anal. Chem. 86: 3525–3532.
519. Gonzales, G.B., Smagghe, G., Raes, K., and Van Camp, J. (2014). Combined alkaline hydrolysis and ultrasound-assisted extraction for the release of nonextractable phenolics from cauliflower (Brassica oleracea var. botrytis) waste. J. Agric. Food Chem. 62: 3371–3376.
520. Wilkerson, C.G. et al. (2014). Monolignol ferulate transferase introduces chemically labile linkages into the lignin backbone. Science 344: 90–93.
2014年4月25日金曜日
2014年4月18日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
511. Morita, Y. et al. (2014). A chalcone isomerase-like protein enhances flavonoid production and flower pigmentation. Plant J. 78: 294–304.
512. Qi, T. et al. Arabidopsis DELLA and JAZ proteins bind the WD-repeat/bHLH/MYB complex to modulate gibberellin and jasmonate signaling synergy. Plant Cell in press.
513. Taniguchi, S. et al. (2014). Isolation of jasmonate-induced sesquiterpene synthase of rice: Product of which has an antifungal activity against Magnaporthe oryzae. J. Plant Physiol. 171: 625–632.
514. Gamir, J., Pastor, V., Kaever, A., Cerezo, M., and Flors, V. (2014). Targeting novel chemical and constitutive primed metabolites against Plectosphaerella cucumerina. Plant J. 78: 227–240.
515. Fan, M. et al. (2014). The bHLH transcription factor HBI1 mediates the trade-off between growth and pathogen-associated molecular pattern–triggered immunity in Arabidopsis. Plant Cell 26: 828–841.
511. Morita, Y. et al. (2014). A chalcone isomerase-like protein enhances flavonoid production and flower pigmentation. Plant J. 78: 294–304.
512. Qi, T. et al. Arabidopsis DELLA and JAZ proteins bind the WD-repeat/bHLH/MYB complex to modulate gibberellin and jasmonate signaling synergy. Plant Cell in press.
513. Taniguchi, S. et al. (2014). Isolation of jasmonate-induced sesquiterpene synthase of rice: Product of which has an antifungal activity against Magnaporthe oryzae. J. Plant Physiol. 171: 625–632.
514. Gamir, J., Pastor, V., Kaever, A., Cerezo, M., and Flors, V. (2014). Targeting novel chemical and constitutive primed metabolites against Plectosphaerella cucumerina. Plant J. 78: 227–240.
515. Fan, M. et al. (2014). The bHLH transcription factor HBI1 mediates the trade-off between growth and pathogen-associated molecular pattern–triggered immunity in Arabidopsis. Plant Cell 26: 828–841.
2014年4月11日金曜日
日本農芸化学会 2014 年度大会，新年度，輪読
3/27～30 に川崎市の明治大学生田キャンパスなどで日本農芸化学会 2014 年度大会が開催されました．本研究室からは修士課程の濱名君と私がそれぞれ「イネのフィトアレキシンであるサクラネチンのいもち病菌による代謝」，「紫外線照射イネ葉からの新規ファイトカサン類縁体の同定」というタイトルで口頭発表を行いました．農芸化学会の発表は，一昨年は各自の PC を接続してプロジェクターで行い，昨年は紙に出力したものを OHC で映写して行いましたが，今年は USB メモリでパワーポイントファイルを持って行って，会場の PC を使う形式になりました．私が見ていた会場では USB メモリの認識に時間がかかって若干遅れたりしたときはありましたが，概ね順調に発表は行われていました．濱名君の発表は，事前のチェックや発表練習では気が付かなかったちょっと恥ずかしいスライドの間違いもありましたが，まあ何とか終了しました．
4 月になりましたので，年度が切り替わりました．今年度の研究室メンバーは博士課程 2 年生 1 名，修士課程 2 年生 1 名，1 年生 1 名，学部 4 年生 4 名の計 6 名となりました．人数的には昨年より 1 名増です．
輪読は今週から再開し，下記の論文を取り上げました．
506. Kawai, Y., Ono, E., and Mizutani, M. (2014). Evolution and diversity of the 2–oxoglutarate-dependent dioxygenase superfamily in plants. Plant J. 78: 328–343.
507. Wölwer-Rieck, U., May, B., Lankes, C., and Wüst, M. (2014). Methylerythritol and mevalonate pathway contributions to biosynthesis of mono-, sesqui-, and diterpenes in glandular trichomes and leaves of Stevia rebaudiana Bertoni. J. Agric. Food Chem. 62: 2428–2435.
508. Ke, Y., Liu, H., Li, X., Xiao, J., and Wang, S. Rice OsPAD4 functions differently from Arabidopsis AtPAD4 in host-pathogen interactions. Plant J. in press.
509. Chassy, A.W., Adams, D.O., and Waterhouse, A.L. (2014). Tracing phenolic metabolism in Vitis vinifera berries with 13C6-phenylalanine: implication of an unidentified intermediate reservoir. J. Agric. Food Chem. 62: 2321–2326.
510. Gaquerel, E., Gulati, J., and Baldwin, I.T. Revealing insect herbivory-induced phenolamide metabolism: from single genes to metabolic network plasticity analysis. Plant J. in press.
4 月になりましたので，年度が切り替わりました．今年度の研究室メンバーは博士課程 2 年生 1 名，修士課程 2 年生 1 名，1 年生 1 名，学部 4 年生 4 名の計 6 名となりました．人数的には昨年より 1 名増です．
輪読は今週から再開し，下記の論文を取り上げました．
506. Kawai, Y., Ono, E., and Mizutani, M. (2014). Evolution and diversity of the 2–oxoglutarate-dependent dioxygenase superfamily in plants. Plant J. 78: 328–343.
507. Wölwer-Rieck, U., May, B., Lankes, C., and Wüst, M. (2014). Methylerythritol and mevalonate pathway contributions to biosynthesis of mono-, sesqui-, and diterpenes in glandular trichomes and leaves of Stevia rebaudiana Bertoni. J. Agric. Food Chem. 62: 2428–2435.
508. Ke, Y., Liu, H., Li, X., Xiao, J., and Wang, S. Rice OsPAD4 functions differently from Arabidopsis AtPAD4 in host-pathogen interactions. Plant J. in press.
509. Chassy, A.W., Adams, D.O., and Waterhouse, A.L. (2014). Tracing phenolic metabolism in Vitis vinifera berries with 13C6-phenylalanine: implication of an unidentified intermediate reservoir. J. Agric. Food Chem. 62: 2321–2326.
510. Gaquerel, E., Gulati, J., and Baldwin, I.T. Revealing insect herbivory-induced phenolamide metabolism: from single genes to metabolic network plasticity analysis. Plant J. in press.
2014年3月20日木曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．学会や年度の切り替わり時期で学生さんが少ないため，輪読は二週間お休みして，4/7 再開予定です．
503. Hubert, J. et al. (2014). Identification of natural metabolites in mixture: a pattern recognition strategy based on 13C NMR. Anal. Chem. 86: 2955–2962.
504. Prince, D.C., Drurey, C., Zipfel, C., and Hogenhout, S. The leucine-rich repeat receptor-like kinase BAK1 and the cytochrome P450 PAD3 contribute to innate immunity to aphids in Arabidopsis. Plant Physiol. in press.
505. Gulati, J., Baldwin, I.T., and Gaquerel, E. The roots of plant defenses: integrative multivariate analyses uncover dynamic behaviors of gene and metabolic networks of roots elicited by leaf herbivory. Plant J. in press.
503. Hubert, J. et al. (2014). Identification of natural metabolites in mixture: a pattern recognition strategy based on 13C NMR. Anal. Chem. 86: 2955–2962.
504. Prince, D.C., Drurey, C., Zipfel, C., and Hogenhout, S. The leucine-rich repeat receptor-like kinase BAK1 and the cytochrome P450 PAD3 contribute to innate immunity to aphids in Arabidopsis. Plant Physiol. in press.
505. Gulati, J., Baldwin, I.T., and Gaquerel, E. The roots of plant defenses: integrative multivariate analyses uncover dynamic behaviors of gene and metabolic networks of roots elicited by leaf herbivory. Plant J. in press.
2014年3月14日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
498. Xin, G.-Z. et al. Rapid identification of plant materials by wooden-tip electrospray ionization mass spectrometry and a strategy to differentiate the bulbs of Fritillaria. Anal. Chim. Acta in press.
499. Xu, T. et al. (2014). Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Science 343: 1025–1028.
500. Rojas, C.M., Senthil-Kumar, M., Tzin, V., and Mysore, K. (2014). Regulation of primary plant metabolism during plant-pathogen interactions and its contribution to plant defense. Front. Plant Sci. 5: 17.
501. Kang, J.-H. et al. (2014). The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato. Plant Physiol. 164: 1161–1174.
502. Aerts, J.T. et al. Patch clamp electrophysiology and capillary electrophoresis–mass spectrometry metabolomics for single cell characterization. Anal. Chem. in press.
498. Xin, G.-Z. et al. Rapid identification of plant materials by wooden-tip electrospray ionization mass spectrometry and a strategy to differentiate the bulbs of Fritillaria. Anal. Chim. Acta in press.
499. Xu, T. et al. (2014). Cell surface ABP1-TMK auxin-sensing complex activates ROP GTPase signaling. Science 343: 1025–1028.
500. Rojas, C.M., Senthil-Kumar, M., Tzin, V., and Mysore, K. (2014). Regulation of primary plant metabolism during plant-pathogen interactions and its contribution to plant defense. Front. Plant Sci. 5: 17.
501. Kang, J.-H. et al. (2014). The flavonoid biosynthetic enzyme chalcone isomerase modulates terpenoid production in glandular trichomes of tomato. Plant Physiol. 164: 1161–1174.
502. Aerts, J.T. et al. Patch clamp electrophysiology and capillary electrophoresis–mass spectrometry metabolomics for single cell characterization. Anal. Chem. in press.
2014年3月8日土曜日
卒論発表会，新しい冷却水循環装置，輪読
一ヶ月近くブログ更新をさぼってしまいました．この間，2/13, 14 に資源生物科学科の卒業論文発表会が開催されて，生物制御化学研究室の 4 名の四年生も無事に発表を終えました．例年のことですが，発表自体は練習の成果もあってそれなりに立派にできていましたが，質疑応答に関しては当日の緊張や日頃の勉強不足の影響でもうひと頑張りが必要だったように感じました．4 名中 3 名は就職，1 名は大学院進学予定ですが，卒論での経験を次の進路でも生かして頑張っていただきたいと思います．
2/10-3/7 の輪読では下記の論文を取り上げました．
485. Van Oystaeyen, A. et al. (2014). Conserved class of queen pheromones stops social insect workers from reproducing. Science 343: 287–290.
486. Liu, Y. et al. Quantification of a botanical negative marker without an identical standard: ginkgotoxin in Ginkgo biloba. J. Nat. Prod. in press.
487. Schmelz, E.A. et al. Biosynthesis, elicitation and roles of monocot terpenoid phytoalexins. Plant J. in press.
488. Kästner, J. et al. (2014). Salicylic acid, a plant defense hormone, is specifically secreted by a molluscan herbivore. PLoS ONE 9: e86500.
489. Song, Y.Y. et al. (2014). Hijacking common mycorrhizal networks for herbivore-induced defence signal transfer between tomato plants. Sci. Rep. 4: 3915.
490. Nagel, R., Berasategui, A., Paetz, C., Gershenzon, J., and Schmidt, A. (2014). Overexpression of an isoprenyl diphosphate synthase in spruce leads to unexpected terpene diversion products that function in plant defense. Plant Physiol. 164: 555–569.
491. Shah, J., Chaturvedi, R., Chowdhury, Z., Venables, B., and Petros, R.A. Signaling by small metabolites in systemic acquired resistance. Plant J. in press.
492. Taurino, M. et al. (2014). Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors. Plant J. 77: 418–429.
493. Sharfman, M., Bar, M., Schuster, S., Leibman, M., and Avni, A. (2014). Sterol-dependent induction of plant defense responses by a microbe-associated molecular pattern from Trichoderma viride. Plant Physiol. 164: 819–827.
494. Gika, H., Wilson, I., and Theodoridis, G. LC-MS-based holistic metabolic profiling. Problems, limitations, advantages, and future perspectives. J. Chromatogr. B in press.
495. VWang, W., Xie, Z.-P., and Staehelin, C. Functional analysis of chimeric LysM domain receptors mediating Nod factor-induced defense signaling in Arabidopsis thaliana and chitin-induced nodulation signaling in Lotus japonicus. Plant J. in press.
496. Boycheva, S., Daviet, L., Wolfender, J.-L., and Fitzpatrick, T.B. The rise of operon-like gene clusters in plants. Trends Plant Sci. in press.
497. LeBrun, E.G., Jones, N.T., and Gilbert, L.E. (2014). Chemical warfare among invaders: a detoxification interaction facilitates an ant invasion. Science 343: 1014–1017.
一研でロータリーエバポレーターや遠心型エバポレーターのために使っている冷却水循環装置は非常に古い器械でもうモーターは使えなくなってしまっていて，別な循環装置を送液に利用することによって，だましだまし使っていました（こちらの記事参照）．何とか予算の目処が立ったので，この装置を更新することにして，2/22 に新しい冷却水循環装置が納品されました．東京理化の CA-1115A という装置です．従来の装置に比べると非常に音が静かで省スペースになりました．
485. Van Oystaeyen, A. et al. (2014). Conserved class of queen pheromones stops social insect workers from reproducing. Science 343: 287–290.
486. Liu, Y. et al. Quantification of a botanical negative marker without an identical standard: ginkgotoxin in Ginkgo biloba. J. Nat. Prod. in press.
487. Schmelz, E.A. et al. Biosynthesis, elicitation and roles of monocot terpenoid phytoalexins. Plant J. in press.
488. Kästner, J. et al. (2014). Salicylic acid, a plant defense hormone, is specifically secreted by a molluscan herbivore. PLoS ONE 9: e86500.
489. Song, Y.Y. et al. (2014). Hijacking common mycorrhizal networks for herbivore-induced defence signal transfer between tomato plants. Sci. Rep. 4: 3915.
490. Nagel, R., Berasategui, A., Paetz, C., Gershenzon, J., and Schmidt, A. (2014). Overexpression of an isoprenyl diphosphate synthase in spruce leads to unexpected terpene diversion products that function in plant defense. Plant Physiol. 164: 555–569.
491. Shah, J., Chaturvedi, R., Chowdhury, Z., Venables, B., and Petros, R.A. Signaling by small metabolites in systemic acquired resistance. Plant J. in press.
492. Taurino, M. et al. (2014). Jasmonate-dependent modifications of the pectin matrix during potato development function as a defense mechanism targeted by Dickeya dadantii virulence factors. Plant J. 77: 418–429.
493. Sharfman, M., Bar, M., Schuster, S., Leibman, M., and Avni, A. (2014). Sterol-dependent induction of plant defense responses by a microbe-associated molecular pattern from Trichoderma viride. Plant Physiol. 164: 819–827.
494. Gika, H., Wilson, I., and Theodoridis, G. LC-MS-based holistic metabolic profiling. Problems, limitations, advantages, and future perspectives. J. Chromatogr. B in press.
495. VWang, W., Xie, Z.-P., and Staehelin, C. Functional analysis of chimeric LysM domain receptors mediating Nod factor-induced defense signaling in Arabidopsis thaliana and chitin-induced nodulation signaling in Lotus japonicus. Plant J. in press.
496. Boycheva, S., Daviet, L., Wolfender, J.-L., and Fitzpatrick, T.B. The rise of operon-like gene clusters in plants. Trends Plant Sci. in press.
497. LeBrun, E.G., Jones, N.T., and Gilbert, L.E. (2014). Chemical warfare among invaders: a detoxification interaction facilitates an ant invasion. Science 343: 1014–1017.
2014年2月10日月曜日
先週および先々週の輪読
先週および先々週の輪読では下記の論文を取り上げました．
475. Nakabayashi, R. et al. (2014). Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids. Plant J. 77: 367–379.
476. Irmisch, S. et al. (2013). Two herbivore-induced cytochrome P450 enzymes CYP79D6 and CYP79D7 catalyze the formation of volatile aldoximes involved in poplar defense. Plant Cell 25: 4737–4754.
477. Choi, J.-H. et al. (2014). The source of “fairy rings”: 2-azahypoxanthine and its metabolite found in a novel purine metabolic pathway in plants. Angew. Chem. Int. Ed. 53: 1552–1555.
478. Hayafune, M. et al. (2014). Chitin-induced activation of immune signaling by the rice receptor CEBiP relies on a unique sandwich-type dimerization. Proc. Natl. Acad. Sci. USA 111: E404–E413.
479. Liu, Y., Wu, Z., Feng, S., Yang, X., and Huang, D. (2014). Hormesis of glyceollin I, an induced phytoalexin from soybean, on budding yeast chronological lifespan extension. Molecules 19: 568–580.
480. Jiang, L. et al. (2013). DWARF 53 acts as a repressor of strigolactone signalling in rice. Nature 504: 401–405.
481. Zhou, F. et al. (2013). D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling. Nature 504: 406–410.
482. Havaux, M. Carotenoid oxidation products as stress signals in plants. Plant J. in press.
483. Smith, J.M., Salamango, D.J., Leslie, M.E., Collins, C.A., and Heese, A. (2014). Sensitivity to flg22 is modulated by ligand-induced degradation and de novo synthesis of the endogenous flagellin-receptor FLAGELLIN-SENSING2. Plant Physiol. 164: 440–454.
484. Waters, M.T., Scaffidi, A., Sun, Y.K., Flematti, G.R., and Smith, S.M. The karrikin response system of Arabidopsis. Plant J. in press.
475. Nakabayashi, R. et al. (2014). Enhancement of oxidative and drought tolerance in Arabidopsis by overaccumulation of antioxidant flavonoids. Plant J. 77: 367–379.
476. Irmisch, S. et al. (2013). Two herbivore-induced cytochrome P450 enzymes CYP79D6 and CYP79D7 catalyze the formation of volatile aldoximes involved in poplar defense. Plant Cell 25: 4737–4754.
477. Choi, J.-H. et al. (2014). The source of “fairy rings”: 2-azahypoxanthine and its metabolite found in a novel purine metabolic pathway in plants. Angew. Chem. Int. Ed. 53: 1552–1555.
478. Hayafune, M. et al. (2014). Chitin-induced activation of immune signaling by the rice receptor CEBiP relies on a unique sandwich-type dimerization. Proc. Natl. Acad. Sci. USA 111: E404–E413.
479. Liu, Y., Wu, Z., Feng, S., Yang, X., and Huang, D. (2014). Hormesis of glyceollin I, an induced phytoalexin from soybean, on budding yeast chronological lifespan extension. Molecules 19: 568–580.
480. Jiang, L. et al. (2013). DWARF 53 acts as a repressor of strigolactone signalling in rice. Nature 504: 401–405.
481. Zhou, F. et al. (2013). D14-SCFD3-dependent degradation of D53 regulates strigolactone signalling. Nature 504: 406–410.
482. Havaux, M. Carotenoid oxidation products as stress signals in plants. Plant J. in press.
483. Smith, J.M., Salamango, D.J., Leslie, M.E., Collins, C.A., and Heese, A. (2014). Sensitivity to flg22 is modulated by ligand-induced degradation and de novo synthesis of the endogenous flagellin-receptor FLAGELLIN-SENSING2. Plant Physiol. 164: 440–454.
484. Waters, M.T., Scaffidi, A., Sun, Y.K., Flematti, G.R., and Smith, S.M. The karrikin response system of Arabidopsis. Plant J. in press.
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