先週の水曜日から外国人研究者としてインドネシアのウダヤナ大学講師の Wiwik さんが生物制御化学研究室に滞在することになりました．Wiwik さんは大学教員であると同時に博士課程の学生でもあり，博士論文をまとめるために茨城大学で機器分析を用いた天然有機化合物の構造解析を行う予定です．月曜日には研究室の学生さんたちとささやかな歓迎会を霞光荘で行いました．日本語の話せない外国人研究者を受入れるのは生物制御化学研究室としては久しぶりです．学生さんたちは（長谷川も）貧しい英会話能力で何とかコミュニケーションを取らなければならないので苦労していますが，そういう経験も良い刺激になると思います．Wiwik さんの滞在期間は三か月と短いですが，是非博士論文に使える良いデータを出してもらいたいと思います．
9/22, 23 の土日には化学生態学研究室と合同で鬼怒川温泉に研究室旅行に出かけました．戸嶋先生と何人かの学生さんは都合が合わずに不参加でしたが，総勢 12 名での旅行となりました．初日はお昼に宇都宮餃子を食べてから，鬼怒川温泉の伊東園ホテルニューさくらにチェックインしました．バイキングでの夕食を取った後は，温泉，麻雀，トランプ，部屋飲みなどで夜の時間を過ごしました．二日目は天候が悪くかなり雨が降っていたのですが，一本杉渓流魚センターというところの釣り堀でニジマス釣りを行いました．あまりに雨が激しかったので，釣りたい人に釣りは任せて，私も含めて半分ぐらいの人は屋根のあるバーベキュー場で雨宿りしていました．釣り組がびしょ濡れになりながら魚を釣った後は串に刺した魚を炭火で焼いて食べました．14:30 過ぎに渓流魚センターを出て，途中道の駅にのみや（真岡）で休憩して，阿見には 19:00 ごろ帰ってきました．雨に濡れながら釣りをしたせいで，その後風邪を引いた学生さんもいたようです．
今週の輪読では下記の論文を取り上げました．
165. Zhang, L., Oh, Y., Li, H., Baldwin, I.T., and Galis, I. Alternative oxidase in resistance to biotic stresses: Nicotiana attenuata AOX contributes to resistance to a pathogen and a piercing-sucking insect but not Manduca sexta larvae. Plant Physiol. in press.
166. Holčapek, M., Jirásko, R., and Lísa, M. (2012). Recent developments in liquid chromatography–mass spectrometry and related techniques. J. Chromatogr. A 1259: 3–15.
167. Soler, R., Erb, M., and Kaplan, I. Long distance root–shoot signalling in plant–insect community interactions. Trends Plant Sci. in press.
168. Sturm, S., and Seger, C. (2012). Liquid chromatography–nuclear magnetic resonance coupling as alternative to liquid chromatography–mass spectrometry hyphenations: Curious option or powerful and complementary routine tool? J. Chromatogr. A 1259: 50–61.
169. Vrkoslav, V., and Cvačka, J. (2012). Identification of the double-bond position in fatty acid methyl esters by liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry. J. Chromatogr. A 1259: 244–250.
2012年9月28日金曜日
2012年9月21日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
161. Li, W. et al. (2012). Ectopic expression of hrf1 enhances bacterial resistance via regulation of diterpene phytoalexins, silicon and reactive oxygen species burst in rice. PLoS ONE 7: e43914.
162. Adelin, E. et al. Biotransformation of natural compounds: Unexpected thio conjugation of Sch-642305 with 3-mercaptolactate catalyzed by Aspergillus niger ATCC 16404 cells. Phytochemistry in press.
163. Khan, I.A., and Smillie, T. Implementing a “quality by design” approach to assure the safety and integrity of botanical dietary supplements. J. Nat. Prod. in press.
164. Milani, N.A., Lawrence, D.P., Elizabeth, A., A., and VanEtten, H.D. Origin of pisatin demethylase (PDA) in the genus Fusarium. Fungal Genet. Biol. in press.
161. Li, W. et al. (2012). Ectopic expression of hrf1 enhances bacterial resistance via regulation of diterpene phytoalexins, silicon and reactive oxygen species burst in rice. PLoS ONE 7: e43914.
162. Adelin, E. et al. Biotransformation of natural compounds: Unexpected thio conjugation of Sch-642305 with 3-mercaptolactate catalyzed by Aspergillus niger ATCC 16404 cells. Phytochemistry in press.
163. Khan, I.A., and Smillie, T. Implementing a “quality by design” approach to assure the safety and integrity of botanical dietary supplements. J. Nat. Prod. in press.
164. Milani, N.A., Lawrence, D.P., Elizabeth, A., A., and VanEtten, H.D. Origin of pisatin demethylase (PDA) in the genus Fusarium. Fungal Genet. Biol. in press.
2012年9月14日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
156. Kusari, S., Pandey, S.P., and Spiteller, M. Untapped mutualistic paradigms linking host plant and endophytic fungal production of similar bioactive secondary metabolites. Phytochemistry in press.
157. Abe, M. et al. Key structural features of cis-cinnamic acid as an allelochemical. Phytochemistry in press.
158. Günnewich, N. et al. A diterpene synthase from the clary sage Salvia sclarea catalyzes the cyclization of geranylgeranyl diphosphate to (8R)-hydroxy-copalyl diphosphate. Phytochemistry in press.
159. De Rybel, B. et al. (2012). A role for the root cap in root branching revealed by the non-auxin probe naxillin. Nat. Chem. Biol. 8: 798–805.
160. Gerbode, S.J., Puzey, J.R., McCormick, A.G., and Mahadevan, L. (2012). How the cucumber tendril coils and overwinds. Science 337: 1087–1091.
156. Kusari, S., Pandey, S.P., and Spiteller, M. Untapped mutualistic paradigms linking host plant and endophytic fungal production of similar bioactive secondary metabolites. Phytochemistry in press.
157. Abe, M. et al. Key structural features of cis-cinnamic acid as an allelochemical. Phytochemistry in press.
158. Günnewich, N. et al. A diterpene synthase from the clary sage Salvia sclarea catalyzes the cyclization of geranylgeranyl diphosphate to (8R)-hydroxy-copalyl diphosphate. Phytochemistry in press.
159. De Rybel, B. et al. (2012). A role for the root cap in root branching revealed by the non-auxin probe naxillin. Nat. Chem. Biol. 8: 798–805.
160. Gerbode, S.J., Puzey, J.R., McCormick, A.G., and Mahadevan, L. (2012). How the cucumber tendril coils and overwinds. Science 337: 1087–1091.
2012年9月7日金曜日
研究室セミナー & 今週の輪読
８月中は夏休みにしていた研究室セミナーを今週から再開しました．９月中は木曜日の午前中に実験報告のみを実施します．
今週の輪読では下記の論文を取り上げました．
151. Gao, Y., Honzatko, R.B., and Peters, R.J. Terpenoid synthase structures: a so far incomplete view of complex catalysis. Nat. Prod. Rep. in press.
152. Zandalinas, S.I., Vives-Peris, V., Gómez-Cadenas, A., and Arbona, V. (2012). A fast and precise method to identify indolic glucosinolates and camalexin in plants by combining mass spectrometric and biological information. J. Agric. Food Chem. 60: 8648–8658.
153. Yamaguchi, H. et al. Phytohormones and willow gall induction by a gall-inducing sawfly. New Phytol. in press.
154. Fujikawa, T. et al. (2012). Surface α-1,3-glucan facilitates fungal stealth infection by interfering with innate immunity in plants. PLoS Pathogens 8: e1002882.
155. He, H. et al. Barnyard grass stress up regulates the biosynthesis of phenolic compounds in allelopathic rice. J. Plant Physiol. in press.
今週の輪読では下記の論文を取り上げました．
151. Gao, Y., Honzatko, R.B., and Peters, R.J. Terpenoid synthase structures: a so far incomplete view of complex catalysis. Nat. Prod. Rep. in press.
152. Zandalinas, S.I., Vives-Peris, V., Gómez-Cadenas, A., and Arbona, V. (2012). A fast and precise method to identify indolic glucosinolates and camalexin in plants by combining mass spectrometric and biological information. J. Agric. Food Chem. 60: 8648–8658.
153. Yamaguchi, H. et al. Phytohormones and willow gall induction by a gall-inducing sawfly. New Phytol. in press.
154. Fujikawa, T. et al. (2012). Surface α-1,3-glucan facilitates fungal stealth infection by interfering with innate immunity in plants. PLoS Pathogens 8: e1002882.
155. He, H. et al. Barnyard grass stress up regulates the biosynthesis of phenolic compounds in allelopathic rice. J. Plant Physiol. in press.
2012年8月31日金曜日
停電 & 今週の輪読
明日は年に一度の電気設備点検のための停電が 9:00～18:00 の間にあります．今日は停電に備えて MS や NMR を停止させました．
今週の輪読では下記の論文を取り上げました．
146. Matsuura, H. et al. Transportation of de novo synthesized jasmonoyl isoleucine in tomato. Phytochemistry in press.
147. Agati, G., Azzarello, E., Pollastri, S., and Tattini, M. Flavonoids as antioxidants in plants: location and functional significance. Plant Sci. in press.
148. Valmalette, J.C. et al. (2012). Light- induced electron transfer and ATP synthesis in a carotene synthesizing insect. Sci. Rep. 2: 579.
149. Tello, E., Castellanos, L., Arévalo-Ferro, C., and Duque, C. Disruption in quorum-sensing systems and bacterial biofilm inhibition by cembranoid diterpenes isolated from the octocoral Eunicea knighti. J. Nat. Prod. in press.
150. Sadanandom, A., Bailey, M., Ewan, R., Lee, J., and Nelis, S. The ubiquitin–proteasome system: central modifier of plant signalling. New Phytol. in press.
今週の輪読では下記の論文を取り上げました．
146. Matsuura, H. et al. Transportation of de novo synthesized jasmonoyl isoleucine in tomato. Phytochemistry in press.
147. Agati, G., Azzarello, E., Pollastri, S., and Tattini, M. Flavonoids as antioxidants in plants: location and functional significance. Plant Sci. in press.
148. Valmalette, J.C. et al. (2012). Light- induced electron transfer and ATP synthesis in a carotene synthesizing insect. Sci. Rep. 2: 579.
149. Tello, E., Castellanos, L., Arévalo-Ferro, C., and Duque, C. Disruption in quorum-sensing systems and bacterial biofilm inhibition by cembranoid diterpenes isolated from the octocoral Eunicea knighti. J. Nat. Prod. in press.
150. Sadanandom, A., Bailey, M., Ewan, R., Lee, J., and Nelis, S. The ubiquitin–proteasome system: central modifier of plant signalling. New Phytol. in press.
2012年8月24日金曜日
今週の輪読
先週は輪読を夏休みにしましたが，今週から再開です．今週の輪読では下記の論文を取り上げました．
141. Caniard, A. et al. (2012). Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture. BMC Plant Biol. 12: 119.
142. Woldemariam, M.G., Onkokesung, N., Baldwin, I.T., and Galis, I. Jasmonoyl-L-isoleucine hydrolase 1 (Jih1) regulates jasmonoyl-L-isoleucine levels and attenuates plant defenses against herbivores. Plant J. in press.
143. Nisha, S. et al. Effect of plant compounds on induced activities of defense-related enzymes and pathogenesis related protein in bacterial blight disease susceptible rice plant. Physiol. Mol. Plant Pathol. in press.
144. Nour-Eldin, H.H. et al. (2012). NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds. Nature 488: 531–534.
145. Aliferis, K.A., and Jabaji, S. (2012). FT-ICR/MS and GC-EI/MS metabolomics networking unravels global potato sprout’s responses to Rhizoctonia solani infection. PLoS ONE 7: e42576.
141. Caniard, A. et al. (2012). Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture. BMC Plant Biol. 12: 119.
142. Woldemariam, M.G., Onkokesung, N., Baldwin, I.T., and Galis, I. Jasmonoyl-L-isoleucine hydrolase 1 (Jih1) regulates jasmonoyl-L-isoleucine levels and attenuates plant defenses against herbivores. Plant J. in press.
143. Nisha, S. et al. Effect of plant compounds on induced activities of defense-related enzymes and pathogenesis related protein in bacterial blight disease susceptible rice plant. Physiol. Mol. Plant Pathol. in press.
144. Nour-Eldin, H.H. et al. (2012). NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds. Nature 488: 531–534.
145. Aliferis, K.A., and Jabaji, S. (2012). FT-ICR/MS and GC-EI/MS metabolomics networking unravels global potato sprout’s responses to Rhizoctonia solani infection. PLoS ONE 7: e42576.
2012年8月12日日曜日
先週の輪読
先週の輪読では下記の論文を取り上げました．火曜日は長谷川も出張で学生さんも一人しか研究室に来ていなかったので，輪読は中止でした．今週は輪読はお休みで，8/20 から再スタートです．
137. Shariatgorji, M. et al. Deuterated matrix-assisted laser desorption ionization matrix uncovers masked mass spectrometry imaging signals of small molecules. Anal. Chem. in press.
138. Wu, S., Peiffer, M., Luthe, D.S., and Felton, G.W. (2012). ATP hydrolyzing salivary enzymes of caterpillars suppress plant defenses. PLoS ONE 7: e41947.
139. Liscombe, D.K., Louie, G.V., and Noel, J.P. Architectures, mechanisms and molecular evolution of natural product methyltransferases. Nat. Prod. Rep. in press.
140. Zuther, K. et al. (2012). Host specificity of Sporisorium reilianum is tightly linked to generation of the phytoalexin luteolinidin by Sorghum bicolor. Mol. Plant Microbe Interact. 25: 1230–1237.
137. Shariatgorji, M. et al. Deuterated matrix-assisted laser desorption ionization matrix uncovers masked mass spectrometry imaging signals of small molecules. Anal. Chem. in press.
138. Wu, S., Peiffer, M., Luthe, D.S., and Felton, G.W. (2012). ATP hydrolyzing salivary enzymes of caterpillars suppress plant defenses. PLoS ONE 7: e41947.
139. Liscombe, D.K., Louie, G.V., and Noel, J.P. Architectures, mechanisms and molecular evolution of natural product methyltransferases. Nat. Prod. Rep. in press.
140. Zuther, K. et al. (2012). Host specificity of Sporisorium reilianum is tightly linked to generation of the phytoalexin luteolinidin by Sorghum bicolor. Mol. Plant Microbe Interact. 25: 1230–1237.
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