三年生の実験開始，前期日程試験，今週の輪読

今週から来年度卒論配属予定の三年生 2 名が研究室に来て練習の実験を始めてもらいました．

2/25（木）は前期日程の個別学力試験が実施されて，大学構内は関係者以外立ち入り禁止となりましたので，研究室はお休みでした．

今週の輪読では下記の論文を取り上げました．三年生は輪読にも参加し始めてもらいました．

898. Bandoly, M., Grichnik, R., Hilker, M., and Steppuhn, A. Priming of anti-herbivore defence in Nicotiana attenuata by insect oviposition: Herbivore specific effects. Plant Cell Environ. in press.

899. Malmierca, M.G. et al. Botrydial and botcinins produced by Botrytis cinerea regulate expression of Trichoderma arundinaceum genes involved in trichothecene biosynthesis. Mol. Plant Pathol. in press.

900. Hrynkiewicz, K., Deka, P., Ruszkiewicz-Michalska, M., Thiem, D., and Szmidt-Jaworska, A. Interactive physiological potential of Peyronellaea curtisii (=Phoma narcissi) strains for enzymatic attack and defence capabilities against phytoalexins. Eur. J. Plant Pathol. in press.

901. Hayashi, K. et al. (2016). Serotonin attenuates biotic stress and leads to lesion browning caused by a hypersensitive response to Magnaporthe oryzae penetration in rice. Plant J. 85: 46–56.

修論・卒論発表会，研究室追いコン＆新歓，輪読

2/9（火）に資源生物科学専攻修士論文発表会が開催されました．生物制御化学研究室からは渡辺美聡さんが発表しました

• 渡辺美聡「KODA類縁体による花芽誘導機構の解明 －プローブ化を指向した新規類縁体の合成研究－」
  

2/15（月）, 16（火）に資源生物科学科卒業論文発表会が開催されました．生物制御化学研究室の四年生 3 名は 2/16 午前中の発表でした．

• 北村遼「植物ホルモン様活性を有する脂肪酸誘導体の合成研究 －12,13位環状修飾型誘導体の合成－」
  
• 福木葵「植物ホルモン様活性を有する脂肪酸誘導体の合成研究 －KODA構造異性体（D-KODA）および新規エステル型誘導体の合成－」
  
• 勝間田駿「イネいもち病菌によるフラボノイド系フィトアレキシン代謝産物の同定」
  

2/16 に開催された農業化学生態学の卒論発表の打ち上げで，投票によって決められたベストプレゼンテーションの第一位に勝間田君の発表が選ばれました．私の記憶によると，生物制御化学研究室の学生が一位になったのは初めてのことだと思います．スライドの字が大きくて読みやすく，話し方も明瞭で確かに良い発表だったと思います．

2/19（金）には生物制御化学研究室の追いコン＆新歓が大学近くの居酒屋たじまで開催されました．今年度は博士 1 名，修士 1 名が修了，四年生 3 名が卒業予定です．来年度は配属予定の新四年生 4 名，修士課程進学予定 2 で，修士課程在学 2 名と合わせて研究室の学生は 8 名になる予定です．

2/8-18 の輪読では下記の論文を取り上げました．

891. Zhai, Q. et al. (2015). Transcriptional mechanism of jasmonate receptor COI1-mediated delay of flowering time in Arabidopsis. Plant Cell 27: 2814–2828.

892. Boachon, B. et al. (2015). CYP76C1 (cytochrome P450)-mediated linalool metabolism and the formation of volatile and soluble linalool oxides in Arabidopsis flowers: a strategy for defense against floral antagonists. Plant Cell 27: 2972–2990.

893. Lee, G., Chung, M.-S., Kang, M., Chung, B., and Lee, S. Direct suppression of a rice bacterial blight (Xanthomonas oryzae pv. oryzae) by monoterpene (S)-limonene. Protoplasma in press.

894. Ding, X. et al. (2015). Priming maize resistance by its neighbours: activating 1,4-benzoxazine-3-ones synthesis and defence gene expression to alleviate leaf disease. Front. Plant Sci. 6: 830.

895. Shabuer, G. et al. (2015). Plant pathogenic anaerobic bacteria use aromatic polyketides to access aerobic territory. Science 350: 670–674.

896. Huang, W.-K., Ji, H.-L., Gheysen, G., and Kyndt, T. Thiamine-induced priming against root-knot nematode infection in rice involves lignification and hydrogen peroxide generation. Mol. Plant Pathol. in press.

897. Blake, S.N., Barry, K.M., Gill, W.M., Reid, J.B., and Foo, E. The role of strigolactones and ethylene in disease caused by Pythium irregulare. Mol. Plant Pathol. in press.

修論・卒論発表直前＆今週の輪読

今週は修士論文発表練習会や予備審査会が開催されて，いよいよ来週 2/9 が修論発表会の本番です．卒業論文発表会は再来週 2/15, 16 に予定されていて，こちらもラストスパートの時期になっています．

今週の輪読では以下の論文を取り上げました．

887. Venturelli, S. et al. (2015). Plants release precursors of histone deacetylase inhibitors to suppress growth of competitors. Plant Cell 27: 3175–3189.

888. Tzin, V. et al. (2015). Dynamic maize responses to aphid feeding are revealed by a time series of transcriptomic and metabolomic assays. Plant Physiol. 169: 1727–1743.

889. Etalo, D.W., De Vos, R.C.H., Joosten, M.H.A.J., and Hall, R.D. (2015). Spatially resolved plant metabolomics: some potentials and limitations of laser-ablation electrospray ionization mass spectrometry metabolite imaging. Plant Physiol. 169: 1424–1435.

890. Gasperini, D. et al. (2015). Axial and radial oxylipin transport. Plant Physiol. 169: 2244–2254.

今週の輪読

今週の輪読では以下の論文を取り上げました．

884. Lee, B., Park, Y.-S., Lee, S., Song, G.C., and Ryu, C.-M. (2016). Bacterial RNAs activate innate immunity in Arabidopsis. New Phytol. 209: 785–797.

885. Wang, G.-F. et al. (2015). Maize homologs of hydroxycinnamoyltransferase, a key enzyme in lignin biosynthesis, bind the nucleotide binding leucine-rich repeat Rp1 proteins to modulate the defense response. Plant Physiol. 169: 2230–2243.

886. Marcos, R. et al. (2015). 9-Lipoxygenase-derived oxylipins activate brassinosteroid signaling to promote cell wall-based defense and limit pathogen infection. Plant Physiol. 169: 2324–2334.

堀江清孝君の学位論文発表会＆2015/12/28-2016/1/22 の輪読

1/21（木）に遺伝子実験施設 1 階の研修セミナー室で連合農学研究科博士課程 3 年の堀江清孝君の学位論文発表会が開催されました．堀江君の研究成果はイネのフィトアレキシンの構造の多様性を明らかにした重要な研究で，博士課程から新たに研究を始めた 3 年間の研究としてはかなり充実した結果が得られています．

2015/12/28〜2016/1/22 の輪読では下記の論文を取り上げました．

871. Soliman, S. et al. (2015). An endophyte constructs fungicide-containing extracellular barriers for its host plant. Curr. Biol. 25: 2570–2576.

872. Pimentel-Elardo, S.M. et al. (2015). Activity-independent discovery of secondary metabolites using chemical elicitation and cheminformatic inference. ACS Chem. Biol. 10: 2616–2623.

873. Cho, J.-G. et al. (2015). Diterpenes from the roots of Oryza sativa L. and their inhibition activity on NO production in LPS-stimulated RAW264.7 macrophages. Chem. Biodiversity 12: 1356–1364.

874. Goto, S. et al. Development of disease-resistant rice by pathogen-responsive expression of WRKY45. Plant Biotechnol. J. in press.

875. Xu, X.-H. et al. (2015). Friend or foe: differential responses of rice to invasion by mutualistic or pathogenic fungi revealed by RNAseq and metabolite profiling. Sci. Rep. 5: 13624.

876. Ueno, Y. et al. (2015). Abiotic stresses antagonize the rice defence pathway through the tyrosine-dephosphorylation of OsMPK6. PLoS Pathogens 11: e1005231.

877. Wolkenstein, K., Sun, H., Falk, H., and Griesinger, C. (2015). Structure and absolute configuration of Jurassic polyketide-derived spiroborate pigments obtained from microgram quantities. J. Am. Chem. Soc. 137: 13460–13463.

878. Fang, M. et al. (2015). Thermal degradation of small molecules: a global metabolomic investigation. Anal. Chem. 87: 10935–10941.

879. Lin, B. et al. (2016). A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system. New Phytol. 209: 1159–1173.

880. Mao, H., Liu, J., Ren, F., Peters, R.J., and Wang, Q. (2016). Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis. Phytochemistry 121: 4–10.

881. García-Cristobal, J., García-Villaraco, A., Ramos, B., Gutierrez-Mañero, J., and Lucas, J.A. (2015). Priming of pathogenesis related-proteins and enzymes related to oxidative stress by plant growth promoting rhizobacteria on rice plants upon abiotic and biotic stress challenge. J. Plant Physiol. 188: 72–79.

882. Li, Y., Kim, J.I., Pysh, L., and Chapple, C. (2015). Four isoforms of Arabidopsis thaliana 4-coumarate: CoA ligase have overlapping yet distinct roles in phenylpropanoid metabolism. Plant Physiol. 169: 2409–2421.

883. Yamamura, C. et al. (2015). Diterpenoid phytoalexin factor, a bHLH transcription factor, plays a central role in the biosynthesis of diterpenoid phytoalexins in rice. Plant J. 84: 1100–1113.

9/7-12/25 の輪読

実に 4 ヶ月近くブログをサボってしまいました．実は長谷川の個人的事情でしばらく更新できない時期があり，溜まってしまうと余計更新作業が億劫になってしまうので，ここまで更新なしになっていた次第です．このまま年を越すのはよろしくないので，とりあえず，輪読の題材のアップから取り掛かります．

この間，実は輪読も 9/12-10/18 の期間は完全にお休みしていますが，これまでの輪読では下記の論文を取り上げました．

823. Venkateshwaran, M. et al. (2015). A role for the mevalonate pathway in early plantsymbiotic signaling. Proc. Natl. Acad. Sci. USA 112: 9781–9786.

824. Mirabella, R. et al. (2015). WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis. Plant J. 83: 1082–1096.

825. Liebeke, M. et al. (2015). Unique metabolites protect earthworms against plant polyphenols. Nat. Commun. 6: 7869.

826. Naessens, E. et al. (2015). A secreted MIF cytokine enables aphid feeding and represses plant immune responses. Curr. Biol. 25: 1898–1903.

827. Zhang, D. et al. (2015). Repression of microRNA biogenesis by silencing of OsDCL1 activates the basal resistance to Magnaporthe oryzae in rice. Plant Sci. 237: 24–32.

828. Conn, C.E. et al. (2015). Convergent evolution of strigolactone perception enabled host detection in parasitic plants. Science 349: 540–543.

829. Yoshimoto, N. et al. (2015). Identification of a flavin-containing S-oxygenating monooxygenase involved in alliin biosynthesis in garlic. Plant J. 83: 941–951.

830. Lori, M. et al. (2015). Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling. J. Exp. Bot. 66: 5315–5325.

831. Plach, M.G., Löffler, P., Merkl, R., and Sterner, R. (2015). Conversion of anthranilate synthase into isochorismate synthase: implications for the evolution of chorismate-utilizing enzymes. Angew. Chem. Int. Ed. 54: 11270–11274.

832. Ng, J.L.P. et al. (2015). Flavonoids and auxin transport inhibitors rescue symbiotic nodulation in the Medicago truncatula cytokinin perception mutant cre1. Plant Cell 27: 2210–2226.

833. Frerot, E., Neirynck, N., Cayeux, I., Yuan, Y.H.-J., and Yuan, Y.-M. (2015). New umami amides: structure–taste relationship studies of cinnamic acid derived amides and the natural occurrence of an intense umami amide in Zanthoxylum piperitum. J. Agric. Food Chem. 63: 7161–7168.

834. Dadashipour, M., Ishida, Y., Yamamoto, K., and Asano, Y. (2015). Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis. Proc. Natl. Acad. Sci. USA 112: 10605–10610.

835. Wang, H. et al. (2015). Rice WRKY4 acts as a transcriptional activator mediating defense responses toward Rhizoctonia solani, the causing agent of rice sheath blight. Plant Mol. Biol. 89: 157–171.

836. Zhang, F. et al. (2015). Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling. Nature 525: 269–273.

837. Ishiga, Y. et al. (2015). Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust. Sci. Rep. 5: 13061.

838. Lemarié, S. et al. (2015). Camalexin contributes to the partial resistance of Arabidopsis thaliana to the biotrophic soilborne protist Plasmodiophora brassicae. Front. Plant Sci. 6: 539.

839. Strugala, R., Delventhal, R., and Schaffrath, U. (2015). An organ-specific view on nonhost resistance. Front. Plant Sci. 6: 526.

840. Galanie, S., Thodey, K., Trenchard, I.J., Filsinger Interrante, M., and Smolke, C.D. (2015). Complete biosynthesis of opioids in yeast. Science 349: 1095–1100.

841. Patkar, R.N. et al. (2015). A fungal monooxygenase-derived jasmonate attenuates host innate immunity. Nat. Chem. Biol. 11: 733–740.

842. Speed, M.P., Fenton, A., Jones, M.G., Ruxton, G.D., and Brockhurst, M.A. (2015). Coevolution can explain defensive secondary metabolite diversity in plants. New Phytol. 208: 1251–1263.

843. Byeon, Y., Choi, G.-H., Lee, H.Y., and Back, K. (2015). Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice. J. Exp. Bot. 66: 6917–6925.

844. Clausen, M. et al. (2015). The bifurcation of the cyanogenic glucoside and glucosinolate biosynthetic pathways. Plant J. 84: 558–573.

845. Rodiuc, N. et al. Evolutionarily distant pathogens require the Arabidopsis phytosulfokine signalling pathway to establish disease. Plant Cell Environ. in press.

846. Kettle, A.J. et al. (2015). A γ-lactamase from cereal infecting Fusarium spp. catalyses the first step in the degradation of the benzoxazolinone class of phytoalexins. Fungal Genet. Biol. 83: 1–9.

847. Hidalgo, W., Chandran, J.N., Menezes, R.C., Otálvaro, F., and Schneider, B. Phenylphenalenones protect banana plants from infection by Mycosphaerella fijiensis and are deactivated by metabolic conversion. Plant Cell Environ. in press.

848. Moghe, G., and Last, R.L. (2015). Something old, something new: Conserved enzymes and the evolution of novelty in plant specialized metabolism. Plant Physiol. 169: 1512–1523.

849. Tsuchiya, Y. et al. (2015). Probing strigolactone receptors in Striga hermonthica with fluorescence. Science 349: 864–868.

850. Olate, V.R., Vélez, M.L., Greslebin, A., and Schmeda-Hirschmann, G. (2015). Phytophthora austrocedri elicitates changes in diterpene profile of Austrocedrus chilensis. Molecules 20: 15084–15097.

851. Falcone Ferreyra, M.L. et al. (2015). The identification of maize and Arabidopsis type I FLAVONE SYNTHASEs links flavones with hormones and biotic interactions. Plant Physiol. 169: 1090–1107.

852. Zheng, S. et al. (2015). Evaluating the effect of expressing a peanut resveratrol synthase gene in rice. PLoS ONE 10: e0136013.

853. Christensen, S.A. et al. (2015). Maize death acids, 9-lipoxygenase–derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators. Proc. Natl. Acad. Sci. USA 112: 11407–11412.

854. Ryffel, F. et al. Metabolic footprint of epiphytic bacteria on Arabidopsis thaliana leaves. ISME J. in press.

855. Zamioudis, C. et al. (2015). Rhizobacterial volatiles and photosynthesis-related signals coordinate MYB72 in Arabidopsis roots during onset of induced systemic resistance and iron deficiency responses. Plant J. 84: 309–322.

856. Hadwiger, L.A. (2015). Nonhost resistance: Self-inflicted DNA damage by fungal DNase accumulation is a major factor in terminating fungal growth in the pea-Fusarium solani f.sp. phaseoli interaction. Physiol. Mol. Plant Pathol. 92: 79–87.

857. Chamam, A., Wisniewski-Dyé, F., Comte, G., Bertrand, C., and Prigent-Combaret, C. (2015). Differential responses of Oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria. Planta 242: 1439–1452.

858. Rasher, D.B. et al. (2015). Marine and terrestrial herbivores display convergent chemical ecology despite 400 million years of independent evolution. Proc. Natl. Acad. Sci. USA 112: 12110–12115.

859. Ray, S. et al. (2015). Maize plants recognize herbivore-associated cues from caterpillar frass. J. Chem. Ecol. 41: 781–792.

860. Li, R. et al. (2015). Prioritizing plant defence over growth through WRKY regulation facilitates infestation by non-target herbivores. eLife 4: e04805.

861. Pedras, M.S.C., Alavi, M., and To, Q.H. (2015). Expanding the nasturlexin family: Nasturlexins C and D and their sulfoxides are phytoalexins of the crucifers Barbarea vulgaris and B. verna. Phytochemistry 118: 131–138.

862. Siciliano, I., Amaral Carneiro, G., Spadaro, D., Garibaldi, A., and Gullino, M. (2015). Jasmonic acid, abscisic acid and salicylic acid are involved in the phytoalexin responses of rice to Fusarium fujikuroi, a high gibberellin producer pathogen. J. Agric. Food Chem. 63: 8134–8142.

863. Xiao, G.-Q., Zhang, H.-W., Lu, X.-Y., and Huang, R.-F. (2015). Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.). J. Integr. Agric. 14: 1687–1696.

864. Alamgir, K.M. et al. Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory. Plant Cell Environ. in press.

865. Qi, J. et al. Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice. J. Integr. Plant Biol. in press.

866. Hu, L. et al. (2015). The rice transcription factor WRKY53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of mitogen-activated protein kinase activity. Plant Physiol. 169: 2907–2921.

867. Scholz, S.S., Reichelt, M., Boland, W., and Mithöfer, A. (2015). Additional evidence against jasmonate-induced jasmonate induction hypothesis. Plant Sci. 239: 9–14.

868. Poloni, A., and Schirawski, J. Host specificity in Sporisorium reilianum is determined by distinct mechanisms in maize and sorghum. Mol. Plant Pathol. in press.

869. Klein, A.P., and Sattely, E.S. (2015). Two cytochromes P450 catalyze S-heterocyclizations in cabbage phytoalexin biosynthesis. Nat. Chem. Biol. 11: 837–839.

870. Poreddy, S. et al. (2015). Detoxification of hostplant/’s chemical defence rather than its anti-predator co-option drives β-glucosidase-mediated lepidopteran counteradaptation. Nat. Commun. 6: 8525.

停電＆今週の輪読

明日の土曜日は毎年行われている電気設備点検のために農学部は朝から夕方まで停電になります．今日は停電に備えて MS や NMR をシャットダウンしました．

今週の輪読では下記の論文を取り上げました．

818. Wang, C. et al. The Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J. in press.

819. Mo, H. et al. Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae. Plant J. in press.

820. Pfeilmeier, S., Saur, I.M.-L., Rathjen, J.P., Zipfel, C., and Malone, J.G. High levels of cyclic-di-GMP in plant-associated Pseudomonas correlate with evasion of plant immunity. Mol. Plant Pathol. in press.

821. Zwack, P.J., and Rashotte, A.M. (2015). Interactions between cytokinin signalling and abiotic stress responses. J. Exp. Bot. 66: 4863–4871.

822. Sripong, K. et al. (2015). Combined treatment with hot water and UV-C elicits disease resistance against anthracnose and improves the quality of harvested mangoes. Crop Prot. 77: 1–8.