茨城大学オープンキャンパス，研究室セミナー，7/12 〜 7/22 の輪読

7/23（土）には水戸キャンパスで茨城大学オープンキャンパスが開催され，生物制御化学研究室の大学院生の勝間田君と北村君が農学部相談コーナーでポスターによる研究発表を行いました．多くの高校生や保護者の皆さんが研究についての説明を熱心に聞かれていました．

7/15 の研究室セミナーの文献紹介では，4 年生の大垣君が下記の論文について発表しました．
Das, S., and Ramana, C.V. (2015). A formal total synthesis of (–)-kumausallene. Tetrahedron 71: 8577–8584.

7/12-7/22 の輪読では下記の論文を取り上げました．

982. Uji, Y. et al. Overexpression of OsMYC2 results in the upregulation of early JA-responsive genes and bacterial blight resistance in rice. Plant Cell Physiol. in press.

983. Maeda, S., Hayashi, N., Sasaya, T., and Mori, M. (2016). Overexpression of BSR1 confers broad-spectrum resistance against two bacterial diseases and two major fungal diseases in rice. Breed. Sci. 66: 396–406.

984. Francisco, M. et al. (2016). The defense metabolite, allyl glucosinolate, modulates Arabidopsis thaliana biomass dependent upon the endogenous glucosinolate pathway. Front. Plant Sci. 7: 774.

985. Wei, Y. et al. (2016). Comparative transcriptional profiling of melatonin synthesis and catabolic genes indicates the possible role of melatonin in developmental and stress responses in rice. Front. Plant Sci. 7: 676.

986. Miyamoto, K. et al. Jasmonoyl-L-isoleucine is required for the production of a flavonoid phytoalexin but not diterpenoid phytoalexins in ultraviolet-irradiated rice leaves. Biosci. Biotechnol. Biochem. in press.

987. Molla, K.A. et al. (2016). Tissue specific expression of Arabidopsis NPR1 gene in rice for sheath blight resistance without compromising phenotypic. Plant Sci. 250: 105–114.

988. Huangfu, J. et al. (2016). The transcription factor OsWRKY45 negatively modulates the resistance of rice to the brown planthopper Nilaparvata lugens. Int. J. Mol. Sci. 17: 697.

989. Willett, C.D. et al. (2016). Benzoxazinone-mediated triazine degradation: a proposed reaction mechanism. J. Agric. Food Chem. 64: 4858–4865.

研究室セミナー & 6/27 〜 7/8 の輪読

7/1 の研究室セミナーの文献紹介では，4 年生の大橋さんが下記の論文について発表しました．
Takeda, N. et al. (2015). Gibberellins interfere with symbiosis signaling and gene expression and alter colonization by arbuscular mycorrhizal fungi in Lotus japonicus. Plant Physiol. 167: 545–557.

7/8 の研究室セミナーの文献紹介では，化学生態学研究室 4 年生の伊與田君が下記の論文について発表しました．
Tatsukami, Y., and Ueda, M. (2016). Rhizobial gibberellin negatively regulates host nodule number. Sci. Rep. 6: 27998.

6/27-7/8 の輪読では下記の論文を取り上げました．

972. Liu, X. et al. (2016). Isolation and identification of potential allelochemicals from aerial parts of Avena fatua L. and their allelopathic effect on wheat. J. Agric. Food Chem. 64: 3492–3500.

973. Akram, W., Anjum, T., and Ali, B. (2016). Phenylacetic acid is ISR determinant produced by Bacillus fortis IAGS162, which involves extensive re-modulation in metabolomics of tomato to protect against Fusarium wilt. Front. Plant Sci. 7: 498.

974. Zhong, Z. et al. (2016). Directional selection from host plants is a major force driving host specificity in Magnaporthe species. Sci. Rep. 6: 25591.

975. Eguchi, Y. et al. (2016). Identification of terpenoids volatilized from Thymus vulgaris L. by heat treatment and their in vitro antimicrobial activity. Physiol. Mol. Plant Pathol. 94: 83–89.

976. Zhou, L.-J. et al. (2016). Antifungal activity of Eucalyptus oil against rice blast fungi and the possible mechanism of gene expression pattern. Molecules 21: 621.

977. Yoshida, K. et al. (2016). Host specialization of the blast fungus Magnaporthe oryzae is associated with dynamic gain and loss of genes linked to transposable elements. BMC Genomics 17: 1–18.

978. Singh, R. et al. (2016). Magnaporthe oryzae effector AVR-Pii helps to establish compatibility by inhibition of the rice NADP-malic enzyme resulting in disruption of oxidative burst and host innate immunity. Mol. Cells 39: 426–438.

979. Lou, Y.-R., Bor, M., Yan, J., Preuss, A.S., and Jander, G. (2016). Arabidopsis NATA1 acetylates putrescine and decreases defense-related hydrogen peroxide accumulation. Plant Physiol. 171: 1443–1455.

980. Liu, J. et al. (2106). Alternative splicing of rice WRKY62 and WRKY76 transcription factor genes in pathogen defense. Plant Physiol. 171: 1427–1442.

981. Kure, A. et al. (2016). Metabolic fate of luteolin in rats: its relationship to anti-inflammatory effect. J. Agric. Food Chem. 64: 4246–4254.

研究室セミナー＆今週の輪読

6/21 の研究室セミナーは都合により 2 名の文献紹介を実施しました．

化学生態学研究室修士 2 年の鈴木君は下記の論文について発表しました．
Li, X.-J. et al. (2016). DWARF overexpression induces alteration in phytohormone homeostasis, development, architecture and carotenoid accumulation in tomato. Plant Biotechnol. J. 14: 1021–1033.

修士 2 年の渡邉さんは下記の論文について発表しました．
Anabuki, T., Tsukahara, M., Matsuura, H., and Takahashi, K. (2016). Tandem photoaffinity labeling of a target protein using a linker with biotin, alkyne and benzophenone groups and a bioactive small molecule with an azide group. Biosci. Biotechnol. Biochem. 80: 432–439.

6/20-24 の輪読では下記の論文を取り上げました．

967. Shibata, Y. et al. The full-size ABCG transporters Nb-ABCG1 and Nb-ABCG2 function in pre- and post-invasion defense against Phytophthora infestans in Nicotiana benthamiana. Plant Cell in press.

968. Miyamoto, K. et al. Evolutionary trajectory of phytoalexin biosynthetic gene clusters in rice. Plant J. in press.

969. Okada, K. et al. (2016). HpDTC1, a stress-inducible bifunctional diterpene cyclase involved in momilactone biosynthesis, functions in chemical defence in the moss Hypnum plumaeforme. Sci. Rep. 6: 25316.

970. Ray, S. et al. (2016). Turnabout is fair play: herbivory-induced plant chitinases excreted in fall armyworm frass suppress herbivore defenses in maize. Plant Physiol. 171: 694–706.

971. Cook, S.D. et al. Auxin biosynthesis: Are the indole-3-acetic acid and phenylacetic acid biosynthesis pathways mirror images. Plant Physiol. in press.

丹羽杯ソフトボール大会，研究室セミナー，5/23 〜 6/17 の輪読

しばらくブログ更新をサボってしまいました．

6/4（土）には学科の研究室対抗ソフトボール大会丹羽杯が開催されました．研究室対抗と言っても，最近は研究室が教員一人一人の単位で運営されていて，研究室単位ではチームが成り立たなくなってしまっているので，チームを作るために適当な合同チームで実施されています．生物制御化学研究室も一研と二研は別のチームに入って参加しています．今年は一研の入っているチームが優勝，二研の入っているチームが準優勝と大変優秀な成績だったようです．

5/26 の研究室セミナーの文献紹介の担当は修士 1 年の北村君で，紹介した論文は Markovič, M., Lopatka, P., Koóš, P., and Gracza, T. (2015). First total synthesis of natural andytriol and a biomimetic approach to varioxiranes. Tetrahedron 71: 8407–8415 でした．6/2 は修士 1 年の勝間田君の担当で，紹介した論文は Piotrowski, J.S. et al. (2015). Plant-derived antifungal agent poacic acid targets β-1,3-glucan. Proc. Natl. Acad. Sci. USA 112: E1490–E1497 でした．6/9 は化学生態学研究室修士 2 年の横山さんの担当で，紹介した論文は Katz, E. et al. (2015). The glucosinolate breakdown product indole-3-carbinol acts as an auxin antagonist in roots of Arabidopsis thaliana. Plant J. 82: 547–555 でした．

5/23-6/17 の輪読では下記の論文を取り上げました．

947. Liu, S., Bartnikas, L.M., Volko, S.M., Ausubel, F.M., and Tang, D. (2016). Mutation of the glucosinolate biosynthesis enzyme cytochrome P450 83A1 monooxygenase increases camalexin accumulation and powdery mildew resistance. Front. Plant Sci. 7: 227.

948. Vannini, C. et al. (2016). An interdomain network: the endobacterium of a mycorrhizal fungus promotes antioxidative responses in both fungal and plant hosts. New Phytol. 211: 265–275.

949. Zhang, T. et al. (2016). Hormone crosstalk in wound stress response: wound-inducible amidohydrolases can simultaneously regulate jasmonate and auxin homeostasis in Arabidopsis thaliana. J. Exp. Bot. 67: 2107–2120.

950. Yamamoto, K. et al. (2016). Cell-specific localization of alkaloids in Catharanthus roseus stem tissue measured with Imaging MS and Single-cell MS. Proc. Natl. Acad. Sci. USA 113: 3891–3896.

951. Yuan, J., Sun, K., Deng-Wang, M.Y., and Dai, C. (2016). The mechanism of ethylene signaling induced by endophytic fungus Gilmaniella sp. AL12 mediating sesquiterpenoids biosynthesis in Atractylodes lancea. Front. Plant Sci. 7: 361.

952. Zhang, N. et al. (2016). Melatonin improved anthocyanin accumulation by regulating gene expressions and resulted in high reactive oxygen species scavenging capacity in cabbage. Front. Plant Sci. 7: 197.

953. Drira, R., and Sakamoto, K. (2016). Sakuranetin induces melanogenesis in B16BL6 melanoma cells through inhibition of ERK and PI3K/AKT signaling pathways. Phytother. Res. 30: 997–1002.

954. Tanabe, K., Hojo, Y., Shinya, T., and Galis, I. Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants. J. Integr. Plant Biol. in press.

955. Choi, H.W. et al. (2016). Activation of plant innate immunity by extracellular High Mobility Group Box 3 and its inhibition by salicylic acid. PLoS Pathog. 12: e1005518.

956. Lan, W. et al. (2016). Maize tricin-oligolignol metabolites and their implications for monocot lignification. Plant Physiol. 171: 810–820.

957. Park, C.H. et al. (2016). The E3 ligase APIP10 connects the effector AvrPiz-t to the NLR receptor Piz-t in rice. PLoS Pathog. 12: e1005529.

958. Marin, A.M. et al. Genetic and functional characterisation of a novel meta-pathway for degradation of naringenin in Herbaspirillum seropedicae SmR1. Environ. Microbiol. in press.

959. Jia, M., Potter, K.C., and Peters, R.J. (2016). Extreme promiscuity of a bacterial and a plant diterpene synthase enables combinatorial biosynthesis. Metab. Eng. 37: 24–34.

960. Llorens, E., Camañes, G., Lapeña, L., and García-Agustín, P. (2016). Priming by Hexanoic acid induce activation of mevalonic and linolenic pathways and promotes the emission of plant volatiles. Front. Plant Sci. 7: 495.

961. Masachis, S. et al. (2016). A fungal pathogen secretes plant alkalinizing peptides to increase infection. Nature Microbiology 1: 16043.

962. Xu, J. et al. (2016). Pathogen-responsive MPK3 and MPK6 reprogram the biosynthesis of indole glucosinolates and their derivatives in Arabidopsis immunity. Plant Cell 28: 1144–1162.

963. Zarattini, M. et al. The bile acid deoxycholate elicits defenses in Arabidopsis and reduces bacterial infection. Mol. Plant Pathol. in press.

964. Fan, S. et al. Identification of phenolic compounds that suppress the virulence of Xanthomonas oryzae on rice via the type III secretion system. Mol. Plant Pathol. in press.

965. Feng, C. et al. (2016). The polygalacturonase-inhibiting protein 4 (OsPGIP4), a potential component of the qBlsr5a locus, confers resistance to bacterial leaf streak in rice. Planta 243: 1297–1308.

966. Cosme, M. et al. A fungal endophyte helps plants to tolerate root herbivory through changes in gibberellin and jasmonate signaling. New Phytol. in press.

研究室セミナー＆ 5/9-20 の輪読

5/2 で今年度の年間計画発表が終了したので，5/12 の研究室セミナーからは文献紹介が始まりました．5/12 は化学生態学研究室博士 1 年の武井さんの担当で，紹介した論文は Christensen, S.A. et al. (2015). Maize death acids, 9-lipoxygenase–derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators. Proc. Natl. Acad. Sci. USA 112: 11407–11412でした．5/19 は修士 2 年の武山君の担当で，紹介した論文は Chanclud, E. et al. (2016). Cytokinin production by the rice blast fungus is a pivotal requirement for full virulence. PLoS Pathoge. 12: e1005457 でした．

5/9-20 の輪読では下記の論文を取り上げました．

938. Zimmermann, M.R., Mithöfer, A., Will, T., Felle, H.H., and Furch, A.C.U. (2016). Herbivore-triggered electrophysiological reactions: candidates for systemic signals in higher plants and the challenge of their identification. Plant Physiol. 170: 2407–2419.

939. Li, L., Chen, X., Ma, C., Wu, H., and Qi, S. (2016). Piriformospora indica requires kaurene synthase activity for successful plant colonization. Plant Physiol. Biochem. 102: 151–160.

940. Sugano, S. et al. (2016). Rice OsVAMP714, a membrane-trafficking protein localized to the chloroplast and vacuolar membrane, is involved in resistance to rice blast disease. Plant Mol. Biol. 91: 81–95.

941. Tsou, L.K. et al. (2016). Antibacterial flavonoids from medicinal plants covalently inactivate type III protein secretion substrates. J. Am. Chem. Soc. 138: 2209–2218.

942. Chanclud, E. et al. (2016). Cytokinin production by the rice blast fungus is a pivotal requirement for full virulence. PLoS Pathog. 12: e1005457.

943. Yi, J. et al. (2016). OsMPK6 plays a critical role in cell differentiation during early embryogenesis in Oryza sativa. J. Exp. Bot. 67: 2425–2437.

944. Takagi, H. et al. (2016). Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner. J. Exp. Bot. 67: 2519–2532.

945. Tian, S. et al. Plant aquaporin AtPIP1;4 links apoplastic H2O2 induction to disease immunity pathways. Plant Physiol. in press.

946. Kumari, C., Dutta, T.K., Banakar, P., and Rao, U. (2016). Comparing the defence-related gene expression changes upon root-knot nematode attack in susceptible versus resistant cultivars of rice. Sci. Rep. 6: 22846.

4/25-5/6 の輪読

4/25-5/6 の輪読では下記の論文を取り上げました．

932. Flamini, R. et al. (2016). Stilbene oligomer phytoalexins in grape as a response to A. carbonarius infection. Physiol. Mol. Plant Pathol. 93: 112–118.

933. Wang, S. et al. (2016). α-Ketol linolenic acid (KODA) application affects endogenous abscisic acid, jasmonic acid and aromatic volatiles in grapes infected by a pathogen (Glomerella cingulata). J. Plant Physiol. 192: 90–97.

934. Dastmalchi, M., Bernards, M., and Dhaubhadel, S. (2016). Twin anchors of the soybean isoflavonoid metabolon: evidence for tethering of the complex to the endoplasmic reticulum by IFS and C4H. Plant J. 85: 689–706.

935. Kim, S.H., Qi, D., Ashfield, T., Helm, M., and Innes, R.W. (2016). Using decoys to expand the recognition specificity of a plant disease resistance protein. Science 351: 684–687.

936. Pelagio-Flores, R., Ruiz-Herrera, L.F., and López-Bucio, J. Serotonin modulates Arabidopsis root growth via changes in reactive oxygen species and jasmonic acid–ethylene signaling. Physiol. Plant. in press.

937. Tamiru, M. et al. A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice. New Phytol. in press.

堀江清孝君の論文が Phytochemistry Letters 誌に掲載．

ブログでの報告が遅れていましたが，この 3 月に博士の学位を取得して連合大学院を修了した堀江清孝君が筆頭著者の論文がヨーロッパ植物化学会の英文誌 Phytochemistry Letters の 2016 年 3 月号に掲載されました．

Horie, K., Sakai, K., Okugi, M., Toshima, H., and Hasegawa, M. (2016). Ultraviolet-induced amides and casbene diterpenoids from rice leaves. Phytochem. Lett. 15: 57–62.

この研究成果は堀江君の博士論文研究の一部ですが，卒業生の酒井健伍君と奥木美咲さんの卒業論文の成果も含まれています．

紫外線照射したイネ葉片に含まれる二次代謝産物を LC/MS を用いてプロファイリングし，従来はイネからは知られていなかった化合物 3 つを発見しました．一つはたの植物からはすでに単離が報告されている N-ベンゾイルチラミンでした．あとの 2 つはカスベン骨格を持つ新規ジテルペン化合物で．2013 年に本研究室の井上靖乃さんが報告したフィトアレキシンである ent-10-オキソデプレッシン の生合成前駆体と考えられるものでした．また，今回発見した化合物を含めて既知のイネフィトアレキシンの蓄積量といもち病菌に対する抗菌活性を再評価し，2 種類のアミド化合物（N-ベンゾイルトリプタミン，N-シナモイルトリプタミン）がいもち病菌に対するフィトアレキシンとして機能していることも明らかにしました．