9/7-12/25 の輪読

実に 4 ヶ月近くブログをサボってしまいました．実は長谷川の個人的事情でしばらく更新できない時期があり，溜まってしまうと余計更新作業が億劫になってしまうので，ここまで更新なしになっていた次第です．このまま年を越すのはよろしくないので，とりあえず，輪読の題材のアップから取り掛かります．

この間，実は輪読も 9/12-10/18 の期間は完全にお休みしていますが，これまでの輪読では下記の論文を取り上げました．

823. Venkateshwaran, M. et al. (2015). A role for the mevalonate pathway in early plantsymbiotic signaling. Proc. Natl. Acad. Sci. USA 112: 9781–9786.

824. Mirabella, R. et al. (2015). WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis. Plant J. 83: 1082–1096.

825. Liebeke, M. et al. (2015). Unique metabolites protect earthworms against plant polyphenols. Nat. Commun. 6: 7869.

826. Naessens, E. et al. (2015). A secreted MIF cytokine enables aphid feeding and represses plant immune responses. Curr. Biol. 25: 1898–1903.

827. Zhang, D. et al. (2015). Repression of microRNA biogenesis by silencing of OsDCL1 activates the basal resistance to Magnaporthe oryzae in rice. Plant Sci. 237: 24–32.

828. Conn, C.E. et al. (2015). Convergent evolution of strigolactone perception enabled host detection in parasitic plants. Science 349: 540–543.

829. Yoshimoto, N. et al. (2015). Identification of a flavin-containing S-oxygenating monooxygenase involved in alliin biosynthesis in garlic. Plant J. 83: 941–951.

830. Lori, M. et al. (2015). Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling. J. Exp. Bot. 66: 5315–5325.

831. Plach, M.G., Löffler, P., Merkl, R., and Sterner, R. (2015). Conversion of anthranilate synthase into isochorismate synthase: implications for the evolution of chorismate-utilizing enzymes. Angew. Chem. Int. Ed. 54: 11270–11274.

832. Ng, J.L.P. et al. (2015). Flavonoids and auxin transport inhibitors rescue symbiotic nodulation in the Medicago truncatula cytokinin perception mutant cre1. Plant Cell 27: 2210–2226.

833. Frerot, E., Neirynck, N., Cayeux, I., Yuan, Y.H.-J., and Yuan, Y.-M. (2015). New umami amides: structure–taste relationship studies of cinnamic acid derived amides and the natural occurrence of an intense umami amide in Zanthoxylum piperitum. J. Agric. Food Chem. 63: 7161–7168.

834. Dadashipour, M., Ishida, Y., Yamamoto, K., and Asano, Y. (2015). Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis. Proc. Natl. Acad. Sci. USA 112: 10605–10610.

835. Wang, H. et al. (2015). Rice WRKY4 acts as a transcriptional activator mediating defense responses toward Rhizoctonia solani, the causing agent of rice sheath blight. Plant Mol. Biol. 89: 157–171.

836. Zhang, F. et al. (2015). Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling. Nature 525: 269–273.

837. Ishiga, Y. et al. (2015). Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust. Sci. Rep. 5: 13061.

838. Lemarié, S. et al. (2015). Camalexin contributes to the partial resistance of Arabidopsis thaliana to the biotrophic soilborne protist Plasmodiophora brassicae. Front. Plant Sci. 6: 539.

839. Strugala, R., Delventhal, R., and Schaffrath, U. (2015). An organ-specific view on nonhost resistance. Front. Plant Sci. 6: 526.

840. Galanie, S., Thodey, K., Trenchard, I.J., Filsinger Interrante, M., and Smolke, C.D. (2015). Complete biosynthesis of opioids in yeast. Science 349: 1095–1100.

841. Patkar, R.N. et al. (2015). A fungal monooxygenase-derived jasmonate attenuates host innate immunity. Nat. Chem. Biol. 11: 733–740.

842. Speed, M.P., Fenton, A., Jones, M.G., Ruxton, G.D., and Brockhurst, M.A. (2015). Coevolution can explain defensive secondary metabolite diversity in plants. New Phytol. 208: 1251–1263.

843. Byeon, Y., Choi, G.-H., Lee, H.Y., and Back, K. (2015). Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice. J. Exp. Bot. 66: 6917–6925.

844. Clausen, M. et al. (2015). The bifurcation of the cyanogenic glucoside and glucosinolate biosynthetic pathways. Plant J. 84: 558–573.

845. Rodiuc, N. et al. Evolutionarily distant pathogens require the Arabidopsis phytosulfokine signalling pathway to establish disease. Plant Cell Environ. in press.

846. Kettle, A.J. et al. (2015). A γ-lactamase from cereal infecting Fusarium spp. catalyses the first step in the degradation of the benzoxazolinone class of phytoalexins. Fungal Genet. Biol. 83: 1–9.

847. Hidalgo, W., Chandran, J.N., Menezes, R.C., Otálvaro, F., and Schneider, B. Phenylphenalenones protect banana plants from infection by Mycosphaerella fijiensis and are deactivated by metabolic conversion. Plant Cell Environ. in press.

848. Moghe, G., and Last, R.L. (2015). Something old, something new: Conserved enzymes and the evolution of novelty in plant specialized metabolism. Plant Physiol. 169: 1512–1523.

849. Tsuchiya, Y. et al. (2015). Probing strigolactone receptors in Striga hermonthica with fluorescence. Science 349: 864–868.

850. Olate, V.R., Vélez, M.L., Greslebin, A., and Schmeda-Hirschmann, G. (2015). Phytophthora austrocedri elicitates changes in diterpene profile of Austrocedrus chilensis. Molecules 20: 15084–15097.

851. Falcone Ferreyra, M.L. et al. (2015). The identification of maize and Arabidopsis type I FLAVONE SYNTHASEs links flavones with hormones and biotic interactions. Plant Physiol. 169: 1090–1107.

852. Zheng, S. et al. (2015). Evaluating the effect of expressing a peanut resveratrol synthase gene in rice. PLoS ONE 10: e0136013.

853. Christensen, S.A. et al. (2015). Maize death acids, 9-lipoxygenase–derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators. Proc. Natl. Acad. Sci. USA 112: 11407–11412.

854. Ryffel, F. et al. Metabolic footprint of epiphytic bacteria on Arabidopsis thaliana leaves. ISME J. in press.

855. Zamioudis, C. et al. (2015). Rhizobacterial volatiles and photosynthesis-related signals coordinate MYB72 in Arabidopsis roots during onset of induced systemic resistance and iron deficiency responses. Plant J. 84: 309–322.

856. Hadwiger, L.A. (2015). Nonhost resistance: Self-inflicted DNA damage by fungal DNase accumulation is a major factor in terminating fungal growth in the pea-Fusarium solani f.sp. phaseoli interaction. Physiol. Mol. Plant Pathol. 92: 79–87.

857. Chamam, A., Wisniewski-Dyé, F., Comte, G., Bertrand, C., and Prigent-Combaret, C. (2015). Differential responses of Oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria. Planta 242: 1439–1452.

858. Rasher, D.B. et al. (2015). Marine and terrestrial herbivores display convergent chemical ecology despite 400 million years of independent evolution. Proc. Natl. Acad. Sci. USA 112: 12110–12115.

859. Ray, S. et al. (2015). Maize plants recognize herbivore-associated cues from caterpillar frass. J. Chem. Ecol. 41: 781–792.

860. Li, R. et al. (2015). Prioritizing plant defence over growth through WRKY regulation facilitates infestation by non-target herbivores. eLife 4: e04805.

861. Pedras, M.S.C., Alavi, M., and To, Q.H. (2015). Expanding the nasturlexin family: Nasturlexins C and D and their sulfoxides are phytoalexins of the crucifers Barbarea vulgaris and B. verna. Phytochemistry 118: 131–138.

862. Siciliano, I., Amaral Carneiro, G., Spadaro, D., Garibaldi, A., and Gullino, M. (2015). Jasmonic acid, abscisic acid and salicylic acid are involved in the phytoalexin responses of rice to Fusarium fujikuroi, a high gibberellin producer pathogen. J. Agric. Food Chem. 63: 8134–8142.

863. Xiao, G.-Q., Zhang, H.-W., Lu, X.-Y., and Huang, R.-F. (2015). Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.). J. Integr. Agric. 14: 1687–1696.

864. Alamgir, K.M. et al. Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory. Plant Cell Environ. in press.

865. Qi, J. et al. Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice. J. Integr. Plant Biol. in press.

866. Hu, L. et al. (2015). The rice transcription factor WRKY53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of mitogen-activated protein kinase activity. Plant Physiol. 169: 2907–2921.

867. Scholz, S.S., Reichelt, M., Boland, W., and Mithöfer, A. (2015). Additional evidence against jasmonate-induced jasmonate induction hypothesis. Plant Sci. 239: 9–14.

868. Poloni, A., and Schirawski, J. Host specificity in Sporisorium reilianum is determined by distinct mechanisms in maize and sorghum. Mol. Plant Pathol. in press.

869. Klein, A.P., and Sattely, E.S. (2015). Two cytochromes P450 catalyze S-heterocyclizations in cabbage phytoalexin biosynthesis. Nat. Chem. Biol. 11: 837–839.

870. Poreddy, S. et al. (2015). Detoxification of hostplant/’s chemical defence rather than its anti-predator co-option drives β-glucosidase-mediated lepidopteran counteradaptation. Nat. Commun. 6: 8525.

停電＆今週の輪読

明日の土曜日は毎年行われている電気設備点検のために農学部は朝から夕方まで停電になります．今日は停電に備えて MS や NMR をシャットダウンしました．

今週の輪読では下記の論文を取り上げました．

818. Wang, C. et al. The Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J. in press.

819. Mo, H. et al. Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae. Plant J. in press.

820. Pfeilmeier, S., Saur, I.M.-L., Rathjen, J.P., Zipfel, C., and Malone, J.G. High levels of cyclic-di-GMP in plant-associated Pseudomonas correlate with evasion of plant immunity. Mol. Plant Pathol. in press.

821. Zwack, P.J., and Rashotte, A.M. (2015). Interactions between cytokinin signalling and abiotic stress responses. J. Exp. Bot. 66: 4863–4871.

822. Sripong, K. et al. (2015). Combined treatment with hot water and UV-C elicits disease resistance against anthracnose and improves the quality of harvested mangoes. Crop Prot. 77: 1–8.

先週の輪読

先週の輪読では下記の論文を取り上げました．

813. Fujisaki, K. et al. (2015). Rice Exo70 interacts with a fungal effector, AVR-Pii and is required for AVR-Pii-triggered immunity. Plant J. 83: 875–887.

814. Zhou, Z. et al. (2015). An Arabidopsis plasma membrane proton ATPase modulates JA signaling and is exploited by the Pseudomonas syringae effector protein AvrB for stomatal invasion. Plant Cell 27: 2032–2041.

815. Manosalva, P. et al. (2015). Conserved nematode signalling molecules elicit plant defenses and pathogen resistance. Nat. Commun. 6: 7795.

816. Benouaret, R., and Goupil, P. (2015). Grape marc extract-induced defense reactions and protection against Phytophthora parasitica are impaired in NahG tobacco plants. J. Agric. Food Chem. 63: 6653–6659.

817. Pruitt, R.N. et al. (2015). The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium. Sci. Adv. 1: e1500245.

全学一斉休業＆輪読

8/12-14 は茨城大学では全学一斉休業日になっていましたので，研究室も基本的にお休みでした．

8/10-20 の輪読では下記の論文を取り上げました．

807. Yahyaa, M. et al. Identification, functional characterization, and evolution of terpene synthases from a basal dicot. Plant Physiol. in press.

808. Ceja-Navarro, J.A. et al. (2015). Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee. Nat. Commun. 6: 7618.

809. Joshi, J.R., Burdman, S., Lipsky, A., Yariv, S., and Yedidia, I. Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum subsp brasiliense via quorum-sensing regulation. Mol. Plant Pathol. in press.

810. Widemann, E. et al. (2015). Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates. Phytochemistry 117: 388–399.

811. Lebeis, S.L. et al. (2015). Salicylic acid modulates colonization of the root microbiome by specific bacterial taxa. Science 349: 860–864.

812. Mou, S. et al. (2015). Over-expression of rice CBS domain containing protein, OsCBSX3, confers rice resistance to Magnaporthe oryzae inoculation. Int. J. Mol. Sci. 16: 15903–15917.

前学期終了＆今週の輪読

茨城大学は 8/7 で前期の期末試験が終了して夏休みに入りました．

今週の輪読では下記の論文を取り上げました．

802. Wang, Y. et al. Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice. J. Exp. Bot. in press.

803. Meharg, C., and Meharg, A.A. (2015). Silicon, the silver bullet for mitigating biotic and abiotic stress, and improving grain quality, in rice. Environ. Exp. Bot. 120: 8–17.

804. Zheng, X. et al. (2015). Spatial and temporal regulation of biosynthesis of the plant immune signal salicylic acid. Proc. Natl. Acad. Sci. USA 112: 9166–9173.

805. Cai, B.-D. et al. (2015). Profiling of phytohormones in rice under elevated cadmium concentration levels by magnetic solid-phase extraction coupled with liquid chromatography tandem mass spectrometry. J. Chromatogr. A 1406: 78–86.

806. Wang, Q. et al. (2015). (E)-β-caryophyllene functions as a host location signal for the rice white-backed planthopper Sogatella furcifera. Physiol. Mol. Plant Pathol. 91: 106–112.

農学部オープンキャンパス，夏休み自由研究相談，大学院入試，研究室セミナー，輪読

しばらくブログ更新をサボってしまいました．世間では夏休みに入っていますが，大学は今が期末試験期間で夏休みは来週からです．

7/18（土）には農学部でオープンキャンパスが開催されました．長谷川は別の業務で研究室見学には対応できなかったのですが，研究室の学生さんたちが高校生と保護者の方に熱心に説明してくれていたようです．

7/30（木）には茨城県教育委員会主催のいばらき理科教育推進事業で小学生の夏休み科学自由研究相談会が農学部で開催され，長谷川と生物制御化学研究室の大学院生 2 名が自由研究の進め方について助言を行いました．必ずしも専門に近くはない分野についての自由研究も多かったので，私も含め院生も事前の下調べが大変でしたが，勉強にはなりました．

8/1（土）には茨城大学大学院農学研究科修士課程の入学試験が実施されました．今年度から資源生物科学選考では英語の試験は外部試験（TOEIC など）のスコア提出に切り替わりましたので，当日の試験は専門科目と面接だけになりました．

7/17 の研究室セミナーでの文献紹介の担当は四年生の福木さんで，紹介した論文は Xu, S., Nguyen, T., Pomilio, I., Vitale, M.C., and Velu, S.E. (2014). Total synthesis of calothrixins A and B via oxidative radical reaction of cyclohexenone with aminophenanthridinedione. Tetrahedron 70: 5928–5933 でした．

7/24 の研究室セミナーでの文献紹介の担当は四年生の佐山君と化学生態学研究室修士二年生の藤原さんで，紹介した論文はそれぞれ Ditengou, F.A. et al. (2015). Volatile signalling by sesquiterpenes from ectomycorrhizal fungi reprogrammes root architecture. Nat. Commun. 6: 6279 と Qu, Y. et al. (2015). Completion of the seven-step pathway from tabersonine to the anticancer drug precursor vindoline and its assembly in yeast. Proc. Natl. Acad. Sci. USA 112: 6224–6229 でした．

7/13-7/30 の輪読では下記の論文を取り上げました．

790. Salas, R.A., Scott, R.C., Dayan, F.E., and Burgos, N.R. (2015). EPSPS gene amplification in glyphosate-resistant italian ryegrass (Lolium perenne ssp. multiflorum) populations from Arkansas (United States). J. Agric. Food Chem. 63: 5885–5893.

791. Cao, W.-L. et al. OsJAMyb, a R2R3-type MYB transcription factor, enhanced blast resistance in transgenic rice. Physiol. Mol. Plant Pathol. in press.

792. Chandler, S. et al. (2015). Role of cyclic lipopeptides produced by Bacillus subtilis in mounting induced immunity in rice (Oryza sativa L.). Physiol. Mol. Plant Pathol. 91: 20–30.

793. Lee, J.S. et al. (2015). Competitive binding of antagonistic peptides fine-tunes stomatal patterning. Nature 522: 439–443.

794. Wu, T. et al. (2015). 3,5-Diarylpyrazole derivatives obtained by ammonolysis of the total flavonoids from Chrysanthemum indicum extract show potential for the treatment of Alzheimer’s disease. J. Nat. Prod. 78: 1593–1599.

795. Winzer, T. et al. (2015). Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein. Science 349: 309–312.

796. Naseem, M., Kaltdorf, M., and Dandekar, T. (2015). The nexus between growth and defence signalling: auxin and cytokinin modulate plant immune response pathways. J. Exp. Bot. 66: 4885–4896.

797. Zerbe, P. et al. Exploring diterpene metabolism in non-model species: Transcriptome-enabled discovery and functional characterization of labda-7,13E-dienyl diphosphate synthase from Grindelia robusta. Plant J. in press.

798. Tamaki, H. et al. (2015). Targeting hormone-related pathways to improve grain yield in rice: a chemical approach. PLoS ONE 10: e0131213.

799. Müller, T.M. et al. (2015). TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE-mediated generation and metabolic analysis of camalexin-deficient cyp71a12 cyp71a13 double knockout lines. Plant Physiol. 168: 849–858.

800. Zhang, B., Xie, M., Bruschweiler-Li, L., Bingol, K., and Brüschweiler, R. (2015). Use of charged nanoparticles in NMR-based metabolomics for spectral simplification and improved metabolite identification. Anal. Chem. 87: 7211–7217.

801. Magnard, J.-L. et al. (2015). Biosynthesis of monoterpene scent compounds in roses. Science 349: 81–83.

研究室セミナー＆今週の輪読

今週の研究室セミナーでの文献紹介の担当は修士課程一年の渡邉さんで，紹介した論文は Toneto Novaes, L.F., Drekener, R.L., Avila, C.M., and Pilli, R.A. (2014). Total synthesis of cryptomoscatones D1 and D2: stereochemical assignment of cryptomoscatone D1. Tetrahedron 70: 6467–6473 でした．細胞分裂阻害活性を持つ植物由来天然物の全合成による絶対立体配置の決定の報告でした．

今週の輪読では下記の論文を取り上げました．

786. Savolainen, O., Pekkinen, J., Katina, K., Poutanen, K., and Hanhineva, K. (2015). Glycosylated benzoxazinoids are degraded during fermentation of wheat bran. J. Agric. Food Chem. 63: 5943–5949.

787. Bandoly, M., Hilker, M., and Steppuhn, A. Oviposition by Spodoptera exigua on Nicotiana attenuata primes induced plant defence against larval herbivory. Plant J. in press.

788. Jin, J. et al. (2015). The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase. J. Exp. Bot. 66: 3959–3975.

789. Aubert, Y., Widemann, E., Miesch, L., Pinot, F., and Heitz, T. (2015). CYP94-mediated jasmonoyl-isoleucine hormone oxidation shapes jasmonate profiles and attenuates defence responses to Botrytis cinerea infection. J. Exp. Bot. 66: 3879–3892.

研究室セミナー＆今週の輪読

今週の研究室セミナーでの文献紹介の担当は化学生態学研究室修士課程一年の横山さんで，紹介した論文は Ma, W. et al. (2014). Auxin biosynthetic gene TAR2 is involved in low nitrogen-mediated reprogramming of root architecture in Arabidopsis. Plant J. 78: 70–79 でした．

今週の輪読では下記の論文を取り上げました．

781. Guo, L. et al. A host plant genome (Zizania latifolia) after a century-long endophyte infection. Plant J. in press.

782. Jiao, X. et al. (2015). Effects of Fusarium solani and F. oxysporum infection on the metabolism of ginsenosides in American ginseng roots. Molecules 20: 10535–10552.

783. Mucha, S., Walther, D., Müller, T.M., Hincha, D.K., and Glawischnig, E. (2015). Substantial reprogramming of the Eutrema salsugineum (Thellungiella salsuginea) transcriptome in response to UV and silver nitrate challenge. BMC Plant Biol. 15: 137.

784. Lam, P.Y., Liu, H., and Lo, C. Completion of tricin biosynthesis pathway in rice: Cytochrome P450 75B4 is a novel chrysoeriol 5’-hydroxylase. Plant Physiol. in press.

785. Cui, G. et al. Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza Bunge. Plant Physiol. in press.

研究室セミナー＆先週の輪読

先週の研究室セミナーでの文献紹介の担当は化学生態学研究室修士課程一年の鈴木君で，紹介した論文は Lan, Z., Krosse, S., Achard, P., van Dam, N.M., and Bede, J.C. (2014). DELLA proteins modulate Arabidopsis defences induced in response to caterpillar herbivory. J. Exp. Bot. 65: 571–583 でした．ジベレリンのシグナル伝達に関わる DELLA タンパク質がシロイヌナズナの食害昆虫に対する防御のシグナル伝達の調節にも関与していることについての報告でした．

先週の輪読では下記の論文を取り上げました．

776. Carvalhais, L.C. et al. Linking jasmonic acid signalling, root exudates and rhizosphere microbiomes. Mol. Plant Microbe Interact. in press.

777. Jisha, V. et al. (2015). Overexpression of an AP2/ERF type transcription factor OsEREBP1 confers biotic and abiotic stress tolerance in rice. PLoS ONE 10: e0127831.

778. Kim, J.I., Dolan, W.L., Anderson, N.A., and Chapple, C. (2015). Indole glucosinolate biosynthesis limits phenylpropanoid accumulation in Arabidopsis thaliana. Plant Cell 27: 1529–1546.

779. Kim, Y., Miyashita, M., and Miyagawa, H. Photocontrol of elicitor activity of PIP-1 to investigate temporal factors involved in phytoalexin biosynthesis. J. Agric. Food Chem. in press.

780. Li, W. et al. The durably resistant rice cultivar Digu activates defense gene expression before the full maturation of Magnaporthe oryzae appressorium. Mol. Plant Pathol. in press.

研究室セミナー＆今週の輪読

今週の研究室セミナーでの文献紹介の担当は修士課程一年の武山君で，紹介した論文は Chen, X.-L. et al. (2014). N-Glycosylation of effector proteins by an α-1,3-mannosyltransferase is required for the rice blast fungus to evade host innate immunity. Plant Cell 26: 1360–1376 でした．

今週の輪読では下記の論文を取り上げました．

771. Song, Z. et al. Heterologous expression of the avirulence gene ACE1 from the fungal rice pathogen Magnaporthe oryzae. Chem. Sci. in press.

772. Miyazaki, S. et al. (2015). Analysis of ent-kaurenoic acid by ultra performance liquid chromatography-tandem mass spectrometry. Biochem. Biophys. Rep. 2: 103–107.

773. Mutuku, J.M. et al. The WRKY45-dependent signaling pathway is required for resistance against Striga parasitism. Plant Physiol. in press.

774. Ferrieri, A.P. et al. A Nicotiana attenuata cell wall invertase inhibitor (NaCWII) reduces growth and increases secondary metabolite biosynthesis in herbivore-attacked plants. New Phytol. in press.

775. Guo, H.-M., Sun, S.-C., Zhang, F.-M., and Miao, X.-X. Identification of genes potentially related to herbivore resistance in OPR3 overexpression rice by microarray analysis. Physiol. Mol. Plant Pathol. in press.

研究室セミナー＆今週の輪読

今週の研究室セミナーでの文献紹介の担当は化学生態学研究室修士課程一年の新井君で，紹介した論文は Kessler, S.C. et al. (2015). Bees prefer foods containing neonicotinoid pesticides. Nature 521: 74–76 でした．ネオニコチノイド系殺虫剤を微量に含む餌についてのハチの嗜好性に関する報告でした．

今週の輪読では下記の論文を取り上げました．

766. Menotta, M., Biagiotti, S., Streppa, L., Rossi, L., and Magnani, M. (2015). Label-free quantification of Tacrolimus in biological samples by atomic force microscopy. Anal. Chim. Acta 884: 90–96.

767. Li, R. et al. (2015). A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X. Sci. Rep. 5: 9682.

768. Mao, X.-M. et al. Epigenetic genome mining of an endophytic fungus leads to the pleiotropic biosynthesis of natural products. Angew. Chem. Int. Ed. in press.

769. Du, B. et al. (2015). Phloem-exudate proteome analysis of response to insect brown plant-hopper in rice. J. Plant Physiol. 183: 13–22.

770. Hazman, M., Hause, B., Eiche, E., Nick, P., and Riemann, M. (2015). Increased tolerance to salt stress in OPDA-deficient rice ALLENE OXIDE CYCLASE mutants is linked to an increased ROS-scavenging activity. J. Exp. Bot. 66: 3339–3352.

研究室セミナー＆今週の輪読

今週の研究室セミナーでの文献紹介の担当は化学生態学研究室修士課程二年の武井さんで，紹介した論文は Ameye, M. et al. (2015). Priming of wheat with the green leaf volatile Z-3-hexenyl acetate enhances defense against Fusarium graminearum but boosts deoxynivalenol production. Plant Physiol. 167: 1671–1684 でした．揮発性化合物によるコムギのフザリウムに対する病害抵抗性の増強に関する報告でした．

今週の輪読では下記の論文を取り上げました．

761. Suzuki, M. et al. (2015). Multiomics in grape berry skin revealed specific induction of the stilbene synthetic pathway by ultraviolet-C irradiation. Plant Physiol. 168: 47–59.

762. Kumar, Y. et al. Metabolic profiling of chickpea-Fusarium interaction identifies differential modulation of disease resistance pathways. Phytochemistry in press.

763. Tsukahara, K. et al. (2015). Ozone-induced rice grain yield loss is triggered via a change in panicle morphology that is controlled by ABERRANT PANICLE ORGANIZATION 1 gene. PLoS ONE 10: e0123308.

764. Lahrmann, U. et al. Mutualistic root endophytism is not associated with the reduction of saprotrophic traits and requires a noncompromised plant innate immunity. New Phytol. in press.

765. Klein, A.T. et al. (2015). Investigation of the chemical interface in the soybean–aphid and rice–bacteria interactions using MALDI-mass spectrometry imaging. Anal. Chem. 87: 5294–5301.

今週の輪読

今週の輪読では下記の論文を取り上げました．

756. Van Bockhaven, J. et al. Primary metabolism plays a central role in moulding silicon-inducible brown spot resistance in rice. Mol. Plant Pathol. in press.

757. Qin, Y., Shen, X., Wang, N., and Ding, X. (2015). Characterization of a novel cyclase-like gene family involved in controlling stress tolerance in rice. J. Plant Physiol. 181: 30–41.

758. Ito, J., Mizuochi, S., Nakagawa, K., Kato, S., and Miyazawa, T. (2015). Tandem mass spectrometry analysis of linoleic and arachidonic acid hydroperoxides via promotion of alkali metal adduct formation. Anal. Chem. 87: 4980–4987.

759. Bartels, S., and Boller, T. Quo vadis, Pep? Plant elicitor peptides at the crossroads of immunity, stress and development. J. Exp. Bot. in press.

760. Tonnessen, B.W. et al. (2015). Rice phenylalanine ammonia-lyase gene OsPAL4 is associated with broad spectrum disease resistance. Plant Mol. Biol. 87: 273–286.

研究室セミナー＆今週の輪読

今週の研究室セミナーでの文献紹介の担当は修士課程二年の渡辺さんで，紹介した論文は Yue, X.-D., Chen, L., and Li, W.-D.Z. (2014). Synthesis and applications of angular acyl-substituted azulenones: formal synthesis of (±)-acorenol and facile access to the hydroazulene core of pseudolaric acid. Tetrahedron 70: 5505–5512 でした．アズレノン誘導体の転位反応を利用した天然物の合成研究についての報告でした．

今週の輪読では下記の論文を取り上げました．

752. Nisa, H. et al. (2015). Fungal endophytes as prolific source of phytochemicals and other bioactive natural products: A review. Microb. Pathog. 82: 50–59.

753. Park, S.-Y. et al. (2015). Agrochemical control of plant water use using engineered abscisic acid receptors. Nature 520: 545–548.

754. Barra, L., Ibrom, K., and Dickschat, J.S. Structural revision and elucidation of the biosynthesis of hypodoratoxide by 13C,13C COSY NMR spectroscopy. Angew. Chem. Int. Ed. in press.

755. Yan, J. et al. (2015). The tyrosine aminomutase TAM1 is required for β-tyrosine biosynthesis in rice. Plant Cell 27: 1265–1278.

阿見オリエンテーション

5/16（土）は農学部の一年生が阿見キャンパスを訪問して大学院生の発表や研究室の見学をする阿見オリエンテーションが開催されました．この行事は一年生向けの教養科目フレッシュマンゼミナール I（主題別ゼミナール）の一部として行われているもので，今年度は長谷川も担当している一年さ生の学生担任が担当しています．

資源生物科学科は全員の学生が出席して，午前中は大学院生 3 名の研究発表（農学セミナー）を聞き，一年生からも多くの質問が出ました．生物制御化学研究室の修士 1 年の渡邉彩音さんにも発表をしてもらいました．午後は学科の研究室をグループごとに訪問して先生や先輩たちの説明を聞いてもらいました．今回のオリエンテーションで集めた情報をもとに来週のフレッシュマンゼミナール I ではグループで発表をしてもらう予定です．

農学セミナーで発表する渡邉彩音さん

真空ポンプ，研究室セミナー，今週の輪読

有機化学系の実験では試料中の溶媒を除去するために真空ポンプで減圧にする操作を多用します．そのための油回転真空ポンプはなくてはならない器械なのですが，先日長年使っていた真空ポンプが電源を入れても動かないという状態になってしまいました．メーカーに引き取って修理の見積をしてもらったところ，修理は可能だったのですが，新品を買うよりちょっと安いぐらいの修理費用がかかるということだったので，結局同等品の新しい真空ポンプを購入しました．年度当初から 20 万円オーバーのかなり痛い出費です．新品はもう届いているのですが，古い真空ポンプを引き取ってもらう時にオイルミストフィルターとかホース接続のためのコネクタとかも一緒に持って行かれてしまったので，それを返してもらうまではまだ使えない状態です．

今週から研究室セミナーでの文献紹介が始まりました．今週の担当は博士課程の堀江君で，紹介した論文は Akagi, A. et al. (2014). WRKY45-dependent priming of diterpenoid phytoalexin biosynthesis in rice and the role of cytokinin in triggering the reaction. Plant Mol. Biol. 86: 171–183 でした．イネのジテルペン系フィトアレキシンの WRKY45 を介したプライミング効果についての報告です．

今週の輪読では下記の論文を取り上げました．

747. Tezuka, D., Ito, A., Mitsuhashi, W., Toyomasu, T., and Imai, R. (2015). The rice ent-KAURENE SYNTHASE LIKE 2 encodes a functional ent-beyerene synthase. Biochem. Biophys. Res. Commun. 460: 766–771.

748. Lan, W. et al. (2015). Tricin, a flavonoid monomer in monocot lignification. Plant Physiol. 167: 1284–1295.

749. Kurotani, K.- et al. (2015). Elevated levels of CYP94 family gene expression alleviate the jasmonate response and enhance salt tolerance in rice. Plant Cell Physiol. 56: 779–789.

750. Soltwisch, J. et al. (2015). Mass spectrometry imaging with laser-induced postionization. Science 348: 211–215.

751. Saint-Macary, M.E. et al. (2015). Methionine biosynthesis is essential for infection in the rice blast fungus Magnaporthe oryzae. PLoS ONE 10: e0111108.

今週の輪読

今週の輪読では下記の論文を取り上げました．
745. Yang, Y.-N. et al. (2015). NMR spectroscopic method for the assignment of 3,5-dioxygenated aromatic rings in natural products. J. Nat. Prod. 78: 705–711.
746. Sun, J. et al. (2015). Characterization and quantitative analysis of phenylpropanoid amides in eggplant (Solanum melongena L.) by high performance liquid chromatography coupled with diode array detection and hybrid ion trap time-of-flight mass spectrometry. J. Agric. Food Chem. 63: 3426–3436.

今週の輪読

今週の輪読では下記の論文を取り上げました．
741. Rahman, A., Wallis, C., and Uddin, W. Silicon induced systemic defense responses in perennial ryegrass against infection by Magnaporthe oryzae. Phytopathology in press.
742. Ditengou, F.A. et al. (2015). Volatile signalling by sesquiterpenes from ectomycorrhizal fungi reprogrammes root architecture. Nat. Commun. 6: 6279.
743. Piotrowski, J.S. et al. (2015). Plant-derived antifungal agent poacic acid targets β-1,3-glucan. Proc. Natl. Acad. Sci. USA 112: E1490–E1497.
744. Tang, W.J., Fernandez, J.G., Sohn, J.J., and Amemiya, C.T. (2015). Chitin is endogenously produced in vertebrates. Curr. Biol. 25: 897–900.

先週と今週の輪読

先週と今週の輪読では下記の論文を取り上げました．
731. Wang, C., Liu, Y., Li, S.-S., and Han, G.-Z. (2015). Insights into the origin and evolution of the plant hormone signaling machinery. Plant Physiol. 167: 872–886.
732. Tsuji, H. et al. (2015). Hd3a promotes lateral branching in rice. Plant J. 82: 256–266.
733. Kellner, F. et al. Genome-guided investigation of plant natural product biosynthesis. Plant J. in press.
734. Li, R.-Y., Wu, X.-M., Yin, X.-H., Long, Y.-H., and Li, M. (2015). Naturally produced citral can significantly inhibit normal physiology and induce cytotoxicity on Magnaporthe grisea. Pestic. Biochem. Physiol. 118: 19–25.
735. Katz, E. et al. The glucosinolate breakdown product indole-3-carbinol acts as an auxin antagonist in roots of Arabidopsis thaliana. Plant J. in press.
736. Kitaoka, N., Wu, Y., Xu, M., and Peters, R. Optimization of recombinant expression enables discovery of novel cytochrome P450 activity in rice diterpenoid biosynthesis. Appl. Microbiol. Biotechnol. in press.
737. Shimizu, T. et al. Live single cell plant hormone analysis by video-mass spectrometry. Plant Cell Physiol. in press.
738. Wang, C. et al. Phytosulfokine is involved in positive regulation of Lotus japonicus nodulation. Mol. Plant Microbe Interact. in press.
739. Li, W. et al. (2015). Overexpressing CYP71Z2 enhances resistance to bacterial blight by suppressing auxin biosynthesis in rice. PLoS ONE 10: e0119867.
740. Sánchez-Martín, J. et al. A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based upon salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism. Plant Cell Environ. in press.

堀江清孝君の論文が J. Agric. Food Chem. 誌に掲載

連合農学研究科博士課程 3 年に在籍している堀江清孝君が筆頭著者の論文がアメリカ化学会の J. Agric. Food Chem. に掲載されました．

Horie, K., Inoue, Y., Sakai, M., Yao, Q., Tanimoto, Y., Koga, J., Toshima, H. and Hasegawa, M. (2015) Identification of UV-induced diterpenes including a new diterpene phytoalexin, phytocassane F, from rice leaves by complementary GC/MS and LC/MS approaches. J. Agric. Food Chem. in press. 

この研究は堀江君だけではなく，本研究室の卒業・修了生の井上靖乃さん，阪井美紀さん，姚群さん，谷本洋輔君の卒論や修論の研究をもとにしている論文です．

イネのフィトアレキシンとしては現在までにジテルペン化合物 15 種類，フラボノイド化合物 1 種類が報告されていました．この論文では新規化合物であるファイトカサン F を紫外線照射イネ葉から精製し，その構造解析を行い，さらに紫外線照射やイネいもち病菌接種による蓄積および抗菌活性を調べて，ファイトカサン F がイネの 17 番目の新規フィトアレキシンであることを明らかにしています．また，同時に抗菌活性は低いものの紫外線照射とイネいもち病菌接種によって蓄積する 2 種のジテルペン化合物の同定も行い，これらがモミラクトン類とオリザレキシン S 生合成における中間体ではないかと推測しています．今回新たに発見されたファイトカサン F は従来から知られていたフィトアレキシンの中で比較的蓄積量も抗菌活性も高いファイトカサン A と同程度の抗菌活性と蓄積量を示しました．したがって，ファイトカサン F は多数あるイネのフィトアレキシンの中でも重要なフィトアレキシンの一つであると考えています．

新年度，堀江君の論文アクセプト，輪読

新年度が始まりました．昨年度の学生は修士 1 名が修了し，4 年生 4 名が卒業しました．今年度は卒業生のうち 2 名が大学院に進学して，新 4 年生 4 名が研究室に配属されました．研究室の学生は博士 3 年 1 名，修士 2 年 1 名，修士 1 年 2 名，学部 4 年 4 名の計 8 名で新年度スタートとなりました．

年度初めから幸先の良いことに博士課程学生の堀江清孝君が筆頭著者の論文が 4/11 にアメリカ化学会の英文誌 J. Agric. Food Chem. にアクセプトされました．この論文は新規のイネフィトアレキシンであるファイトカサン F とジテルペン系フィトアレキシン生合成中間体と想定される 2 つの化合物についての報告です．本研究は，堀江君の博士論文研究の一部ですが，卒業・修了生の井上靖乃さん，阪井美紀さん，姚群さん，谷本洋輔君の卒業・修士論文研究を発展させたものです．詳しい内容については別のエントリで後ほど書こうと思います．
Horie K, Inoue Y, Sakai M, Yao Q, Tanimoto Y, Koga J, Toshima H, and Hasegawa M, Identification of UV-induced diterpenes including a new diterpene phytoalexin, phytocassane F, from rice leaves by complementary GC/MS and LC/MS approaches. J. Agric. Food Chem., in press.

3/16 から 4/10 の輪読では下記の論文を取り上げました．
718. Dhakal, R., Park, E., Lee, S.-W., and Baek, K.-H. (2015). Soybean (Glycine max L. Merr.) sprouts germinated under red light irradiation induce disease resistance against bacterial rotting disease. PLoS ONE 10: e0117712.
719. Monjil, M.S. et al. (2015). Methanol extract of mycelia from Phytophthora infestans-induced resistance in potato. C. R. Biol. 338: 185–196.
720. Shyu, C., and Brutnell, T.P. Growth–defence balance in grass biomass production: the role of jasmonates. J. Exp. Bot. in press.
721. Ameye, M. et al. (2015). Priming of wheat with the green leaf volatile Z-3-hexenyl acetate enhances defense against Fusarium graminearum but boosts deoxynivalenol production. Plant Physiol. 167: 1671–1684.
722. Richardson, L.L. et al. (2015). Secondary metabolites in floral nectar reduce parasite infections in bumblebees. Proc. R. Soc. London, Ser. B 282: 20142471.
723. Jacobo-Velazquez, D.A., Gonzalez-Aguero, M., and Cisneros-Zevallos, L. (2015). Cross-talk between signaling pathways: The link between plant secondary metabolite production and wounding stress response. Sci. Rep. 5: 8608.
724. Sohrabi, R. et al. (2015). In planta variation of volatile biosynthesis: an alternative biosynthetic route to the formation of the pathogen-induced volatile homoterpene DMNT via triterpene degradation in Arabidopsis roots. Plant Cell 27: 874–890.
725. Rasmann, S., Chassin, E., Bilat, J., Glauser, G., and Reymond, P. Trade-off between constitutive and inducible resistance against herbivores is only partially explained by gene expression and glucosinolate production. J. Exp. Bot. in press.
726. Sánchez-Vallet, A., Mesters, J.R., and Thomma, B.P.H.J. (2015). The battle for chitin recognition in plant-microbe interactions. FEMS Microbiol. Rev. 39: 171–183.
727. Lu, J. et al. (2015). Induced jasmonate signaling leads to contrasting effects on root damage and herbivore performance. Plant Physiol. 167: 1100–1116.
728. Asselin, J.A.E. et al. (2015). Perturbation of maize phenylpropanoid metabolism by an AvrE family type III effector from Pantoea stewartii. Plant Physiol. 167: 1117–1135.
729. Rudd, J.J. et al. (2015). Transcriptome and metabolite profiling of the infection cycle of Zymoseptoria tritici on wheat reveals a biphasic interaction with plant immunity involving differential pathogen chromosomal contributions and a variation on the hemibiotrophic lifestyle definition. Plant Physiol. 167: 1158–1185.
730. Neukermans, J. et al. (2015). ARACINs, Brassicaceae-specific peptides exhibiting antifungal activities against necrotrophic pathogens in Arabidopsis. Plant Physiol. 167: 1017–1029.

先週の輪読

先週の輪読では以下の論文を取り上げました．
713. Heinze, M., Brandt, W., Marillonnet, S., and Roos, W. (2015). “Self” and “non-self” in the control of phytoalexin biosynthesis: plant phospholipases A2 with alkaloid-specific molecular fingerprints. Plant Cell 27: 448–462.
714. Erb, M. et al. (2015). Indole is an essential herbivore-induced volatile priming signal in maize. Nat. Commun. 6: 6273.
715. Meguro, A. et al. An unusual terpene cyclization mechanism involving a carbon–carbon bond rearrangement. Angew. Chem. Int. Ed. in press.
716. Kettle, A.J. et al. Degradation of the benzoxazolinone class of phytoalexins is important for virulence of Fusarium pseudograminearum towards wheat. Mol. Plant Pathol. in press.
717. Liang, X.Y. et al. Two thiadiazole compounds promote rice defense against Xanthomonas oryzae pv. oryzae by suppressing the bacterium’s production of extracellular polysaccharides. Mol. Plant Pathol. in press.

今週の輪読

今週の輪読では以下の論文を取り上げました．
709. Piasecka, A., Jedrzejczak-Rey, N., and Bednarek, P. Secondary metabolites in plant innate immunity: conserved function of divergent chemicals. New Phytol. in press.
710. Ji, H. et al. The role of thionins in rice defence against root pathogens. Mol. Plant Pathol. in press.
711. Díaz, L., Del Río, J.A., Pérez-Gilabert, M., and Ortuño, A. (2015). Involvement of an extracellular fungus laccase in the flavonoid metabolism in Citrus fruits inoculated with Alternaria alternata. Plant Physiol. Biochem. 89: 11–17.
712. García, T., Gutiérrez, J., Veloso, J., Gago-Fuentes, R., and Díaz, J. (2015). Wounding induces local resistance but systemic susceptibility to Botrytis cinerea in pepper plants. J. Plant Physiol. 176: 202–209.

三年生の引継実験，前期日程入試，今週の輪読

修論発表会と卒論発表会が終了し，修士二年生と四年生は一段落したところですが，来年度の卒論に配属される予定の学生さんたちが先週ぐらいから引継（練習）実験を始めました．最初のうちは研究室での生活に慣れるのに精一杯で結構疲れると思いますが，頑張って欲しいと思います．

2/25（水）は茨城大学農学部でも前期日程の入学試験が実施されました．

輪読にも三年生が加わりました．英語の専門用語に慣れるまでは結構苦労するはずですが，毎日やっていればすぐによく出てくる単語は覚えます．今週の輪読では以下の論文を取り上げました．
705. Zhao, Q., Wang, M., Xu, D., Zhang, Q., and Liu, W. (2015). Metabolic coupling of two small-molecule thiols programs the biosynthesis of lincomycin A. Nature 518: 115–119.
706. Gries, R. et al. (2015). Bed bug aggregation pheromone finally identified. Angew. Chem. Int. Ed. 54: 1135–1138.
707. Lee, H.Y., Byeon, Y., Tan, D.-X., Reiter, R.J., and Back, K. Arabidopsis serotonin N-acetyltransferase knockout mutant plants exhibit decreased melatonin and salicylic acid levels resulting in susceptibility to an avirulent pathogen. J. Pineal Res. in press.
708. Liu, J. et al. (2015). The RhoGAP SPIN6 associates with SPL11 and OsRac1 and negatively regulates programmed cell death and innate immunity in rice. PLoS Pathogen 11: e1004629.

研究室新歓＆追いコン，今週の輪読

金曜日には大学の近くの居酒屋「たじま」で研究室の新三年生歓迎兼卒業修了生追い出しコンパを開催しました．来年度は 4 名の卒論学生が研究室配属予定で，大学院生 1 名が修了，四年生 4 名が卒業（うち 2 名は修士進学予定）の見込みです．来年度の研究室所属学生は博士 1 名，修士 3 名，四年生 4 名の計 8 名で，今年度からは 1 名増えることになります．

今週の輪読では以下の論文を取り上げました．
702. Wolfender, J.-L., Marti, G., Thomas, A., and Bertrand, S. (2015). Current approaches and challenges for the metabolite profiling of complex natural extracts. J. Chromatogr. A 1382: 136–164.
703. Liu, Z. et al. (2015). Functional diversity of jasmonates in rice. Rice 8: 1–13.
704. Kumar, A. et al. Comparative proteomics reveals differential induction of both biotic and abiotic stress response associated proteins in rice during Xanthomonas oryzae pv. oryzae infection. Funct. Integr. Genomics in press.

卒業論文・修士論文発表会，研究室セミナー，輪読

しばらくブログ更新をサボってしまい，2015 年最初の更新が 2 月半ばとなってしまいました．

2/10 には資源生物科学専攻の修士論文発表会が開催されました．生物制御化学研究室からは濱名一穂君が「イネいもち病菌によるサクラネチンの代謝に関する研究」というタイトルで 2 年間の研究成果を発表しました．2/12, 13 には資源生物科学かの卒業論文発表会も開催され，生物制御化学研究室からは下記の 4 名の四年生が発表を行いました．
　照山真司「花芽誘導活性を有する脂肪酸類縁体の合成研究 - シクロペンタン環導入誘導体の合成 -」
　渡邉彩音「花芽誘導活性を有する脂肪酸類縁体の合成研究 - 芳香環導入誘導体の合成 -」
　酒井健伍「紫外線照射イネ葉に蓄積するアミド化合物に関する研究」
　武山恵典「モミラクトン A のいもち病菌による代謝に関する研究」
武山君の発表はその後に行われた農業化学生態学の打ち上げでの優秀発表の投票で第 2 位の栄誉に輝きました．

12/10 のセミナーでの文献紹介の担当は四年生の渡邉さんで，紹介した論文は Mori, M., Miki, N., Ito, D., Kondo, T. & Yoshida, K. Structure of tecophilin, a tri-caffeoylanthocyanin from the blue petals of Tecophilaea cyanocrocus, and the mechanism of blue color development. Tetrahedron 70, 8657-8664 (2014) でした．Tecophilaea cyanocrocus の花の青色色素であるカフェオイルアントシアニン三量体 tecophilin の構造についての報告でした．

12/17 のセミナーでの文献紹介の担当は化学生態学研究室四年生の横山さんで，紹介した論文は Bailly, A. et al. The inter-kingdom volatile signal indole promotes root development by interfering with auxin signalling. Plant J. 80, 758-771 (2014) でした．土壌細菌が放出する揮発性化合物であるインドールの植物の成長に与える影響についての報告でした．

1/7 のセミナーでの文献紹介の担当は四年生の酒井君で，紹介した論文は Sun, H. et al. Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling. J. Exp. Bot. 65, 4305-4315 (2014) でした．スコポレチンが野生タバコにおいて Alternaria alternata に対するフィトアレキシンとして働くことについての報告でした．

1/14 のセミナーでの文献紹介の担当は四年生の照山君で，紹介した論文は Hayashi, K. et al. A novel total synthesis of isocryptolepine based on a microwave-assisted tandem Curtius rearrangement and aza-electrocyclic reaction. Tetrahedron 68, 4274-4279 (2012) でした．抗マラリア活性のある天然物 isocryptolepine のマイクロ波照射を利用した合成研究についての報告でした．

12/8-2/13 の輪読では下記の論文を取り上げました．
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662. Zhang, X. et al. (2015). The receptor kinase CERK1 has dual functions in symbiosis and immunity signalling. Plant J. 81: 258–267.
663. Vaughan, M.M. et al. The accumulation of terpenoid phytoalexins in maize roots is associated with drought tolerance. Plant Cell Environ. in press.
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666. Seneviratne, H.K. et al. Non-host disease resistance response in pea (Pisum sativum) pods: Biochemical function of DRR206 and phytoalexin pathway localization. Phytochemistry in press.
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670. Stitz, M., Hartl, M., Baldwin, I.T., and Gaquerel, E. (2014). Jasmonoyl-L-isoleucine coordinates metabolic networks required for anthesis and floral attractant emission in wild tobacco (Nicotiana attenuata). Plant Cell 26: 3964–3983.
671. Eugster, P.J. et al. (2014). Retention time prediction for dereplication of natural products (CxHyOz) in LC–MS metabolite profiling. Phytochemistry 108: 196–207.
672. Shang, Y. et al. (2014). Biosynthesis, regulation, and domestication of bitterness in cucumber. Science 346: 1084–1088.
673. Takei, M., Yoshida, S., Kawai, T., Hasegawa, M., and Suzuki, Y. (2015). Adaptive significance of gall formation for a gall-inducing aphids on Japanese elm trees. J. Insect Physiol. 72: 43–51.
674. Fang, C. et al. Identification and comparative analysis of microRNAs in barnyardgrass (Echinochloa crus-galli) in response to rice allelopathy. Plant Cell Environ. in press.
675. Lee, G., Lee, S., Chung, M.-S., Jeong, Y., and Chung, B. Rice terpene synthase 20 (OsTPS20) plays an important role in producing terpene volatiles in response to abiotic stresses. Protoplasma in press.
676. Farmer, E.E., Gasperini, D., and Acosta, I.F. (2014). The squeeze cell hypothesis for the activation of jasmonate synthesis in response to wounding. New Phytol. 204: 282–288.
677. Dai, L. et al. (2015). Design, synthesis, and evaluation of caffeic acid amides as synergists to sensitize fluconazole-resistant Candida albicans to fluconazole. Bioorg. Med. Chem. Lett. 25: 34–37.
678. Wiesel, L. et al. (2014). Molecular effects of resistance elicitors from biological origin and their potential for crop protection. Front. Plant Sci. 5: 655.
679. Goto, S. et al. Development of disease-resistant rice by optimized expression of WRKY45. Plant Biotechnol. J. in press.
680. Boutanaev, A.M. et al. (2015). Investigation of terpene diversification across multiple sequenced plant genomes. Proc. Natl. Acad. Sci. USA 112: E81–E88.
681. Ranjan, A. et al. Upregulation of jasmonate biosynthesis and jasmonate-responsive genes in rice leaves in response to a bacterial pathogen mimic. Funct. Integr. Genomics in press.
682. Shi, L. et al. The regulation of methyl jasmonate on hyphal branching and GA biosynthesis in Ganoderma lucidum partly via ROS generated by NADPH oxidase. Fungal Genet. Biol. in press.
683. Yamada, Y. et al. (2015). Terpene synthases are widely distributed in bacteria. Proc. Natl. Acad. Sci. USA 112: 857–862.
684. Fukuda, A., Sugimoto, K., Ando, T., Yamamoto, T., and Yano, M. Chromosomal locations of a gene underlying heat-accelerated brown spot formation and its suppressor genes in rice. Mol. Genet. Genomics in press.
685. Ramegowda, V., and Senthil-Kumar, M. (2015). The interactive effects of simultaneous biotic and abiotic stresses on plants: Mechanistic understanding from drought and pathogen combination. J. Plant Physiol. 176: 47–54.
686. Hadwiger, L.A., and Tanaka, K. (2014). EDTA a novel inducer of pisatin, a phytoalexin indicator of the non-host resistance in peas. Molecules 20: 24–34.
687. Ling, L.L. et al. (2015). A new antibiotic kills pathogens without detectable resistance. Nature 517: 455–459.
688. Roos, J., Bejai, S., Mozūraitis, R., and Dixelius, C. (2015). Susceptibility to Verticillium longisporum is linked to monoterpene production by TPS23/27 in Arabidopsis. Plant J. 81: 572–585.
689. Andersson, M.X. et al. (2015). Involvement of the electrophilic isothiocyanate sulforaphane in Arabidopsis Local defense responses. Plant Physiol. 167: 251–261.
690. Larrieu, A. et al. (2015). A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants. Nat. Commun. 6: 6043.
691. Narusaka, M. et al. (2015). Yeast cell wall extract induces disease resistance against bacterial and fungal pathogens in Arabidopsis thaliana and Brassica crop. PLoS ONE 10: e0115864.
692. Hu, X. et al. Re-examination of chlorophyllase function implies its involvement in defense against chewing herbivores. Plant Physiol. in press.
693. Shroff, R. et al. Quantification of plant surface metabolites by MALDI mass spectrometry imaging: glucosinolates on Arabidopsis thaliana leaves. Plant J. in press.
694. Berim, A., Kim, M.-J., and Gang, D.R. (2015). Identification of a unique 2-oxoglutarate-dependent flavone 7-O-demethylase completes the elucidation of the lipophilic flavone network in basil. Plant Cell Physiol. 56: 126–136.
695. Ji, H., Kyndt, T., He, W., Vanholme, B., and Gheysen, G. β-aminobutyric acid–induced resistance against root-knot nematodes in rice is based on increased basal defence. Mol. Plant Microbe Interact. in press.
696. Setyaningsih, W., Saputro, I.E., Barbero, G.F., Palma, M., and Garc√≠a, B., Carmelo (2015). Determination of melatonin in rice (Oryza sativa) grains by pressurized liquid extraction. J. Agric. Food Chem. 63: 1107–1115.
697. Hardeland, R. (2015). Melatonin in plants and other phototrophs: advances and gaps concerning the diversity of functions. J. Exp. Bot. 66: 627–646.
698. Wei, W. et al. (2015). Melatonin enhances plant growth and abiotic stress tolerance in soybean plants. J. Exp. Bot. 66: 695–707.
699. Kim, N.H., and Hwang, B.K. (2015). Pepper heat shock protein 70a interacts with the type III effector AvrBsT and triggers plant cell death and immunity. Plant Physiol. 167: 307–322.
700. Van Bockhaven, J. et al. Silicon induces resistance to the brown spot fungus Cochliobolus miyabeanus by preventing the pathogen from hijacking the rice ethylene pathway. New Phytol. in press.
701. Cheng, H. et al. The WRKY45-2–WRKY13–WRKY42 transcriptional regulatory cascade is required for rice resistance to fungal pathogen. Plant Physiol. in press.