堀江清孝君の学位論文発表会＆2015/12/28-2016/1/22 の輪読

1/21（木）に遺伝子実験施設 1 階の研修セミナー室で連合農学研究科博士課程 3 年の堀江清孝君の学位論文発表会が開催されました．堀江君の研究成果はイネのフィトアレキシンの構造の多様性を明らかにした重要な研究で，博士課程から新たに研究を始めた 3 年間の研究としてはかなり充実した結果が得られています．

2015/12/28〜2016/1/22 の輪読では下記の論文を取り上げました．

871. Soliman, S. et al. (2015). An endophyte constructs fungicide-containing extracellular barriers for its host plant. Curr. Biol. 25: 2570–2576.

872. Pimentel-Elardo, S.M. et al. (2015). Activity-independent discovery of secondary metabolites using chemical elicitation and cheminformatic inference. ACS Chem. Biol. 10: 2616–2623.

873. Cho, J.-G. et al. (2015). Diterpenes from the roots of Oryza sativa L. and their inhibition activity on NO production in LPS-stimulated RAW264.7 macrophages. Chem. Biodiversity 12: 1356–1364.

874. Goto, S. et al. Development of disease-resistant rice by pathogen-responsive expression of WRKY45. Plant Biotechnol. J. in press.

875. Xu, X.-H. et al. (2015). Friend or foe: differential responses of rice to invasion by mutualistic or pathogenic fungi revealed by RNAseq and metabolite profiling. Sci. Rep. 5: 13624.

876. Ueno, Y. et al. (2015). Abiotic stresses antagonize the rice defence pathway through the tyrosine-dephosphorylation of OsMPK6. PLoS Pathogens 11: e1005231.

877. Wolkenstein, K., Sun, H., Falk, H., and Griesinger, C. (2015). Structure and absolute configuration of Jurassic polyketide-derived spiroborate pigments obtained from microgram quantities. J. Am. Chem. Soc. 137: 13460–13463.

878. Fang, M. et al. (2015). Thermal degradation of small molecules: a global metabolomic investigation. Anal. Chem. 87: 10935–10941.

879. Lin, B. et al. (2016). A novel nematode effector suppresses plant immunity by activating host reactive oxygen species-scavenging system. New Phytol. 209: 1159–1173.

880. Mao, H., Liu, J., Ren, F., Peters, R.J., and Wang, Q. (2016). Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis. Phytochemistry 121: 4–10.

881. García-Cristobal, J., García-Villaraco, A., Ramos, B., Gutierrez-Mañero, J., and Lucas, J.A. (2015). Priming of pathogenesis related-proteins and enzymes related to oxidative stress by plant growth promoting rhizobacteria on rice plants upon abiotic and biotic stress challenge. J. Plant Physiol. 188: 72–79.

882. Li, Y., Kim, J.I., Pysh, L., and Chapple, C. (2015). Four isoforms of Arabidopsis thaliana 4-coumarate: CoA ligase have overlapping yet distinct roles in phenylpropanoid metabolism. Plant Physiol. 169: 2409–2421.

883. Yamamura, C. et al. (2015). Diterpenoid phytoalexin factor, a bHLH transcription factor, plays a central role in the biosynthesis of diterpenoid phytoalexins in rice. Plant J. 84: 1100–1113.

9/7-12/25 の輪読

実に 4 ヶ月近くブログをサボってしまいました．実は長谷川の個人的事情でしばらく更新できない時期があり，溜まってしまうと余計更新作業が億劫になってしまうので，ここまで更新なしになっていた次第です．このまま年を越すのはよろしくないので，とりあえず，輪読の題材のアップから取り掛かります．

この間，実は輪読も 9/12-10/18 の期間は完全にお休みしていますが，これまでの輪読では下記の論文を取り上げました．

823. Venkateshwaran, M. et al. (2015). A role for the mevalonate pathway in early plantsymbiotic signaling. Proc. Natl. Acad. Sci. USA 112: 9781–9786.

824. Mirabella, R. et al. (2015). WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal in Arabidopsis. Plant J. 83: 1082–1096.

825. Liebeke, M. et al. (2015). Unique metabolites protect earthworms against plant polyphenols. Nat. Commun. 6: 7869.

826. Naessens, E. et al. (2015). A secreted MIF cytokine enables aphid feeding and represses plant immune responses. Curr. Biol. 25: 1898–1903.

827. Zhang, D. et al. (2015). Repression of microRNA biogenesis by silencing of OsDCL1 activates the basal resistance to Magnaporthe oryzae in rice. Plant Sci. 237: 24–32.

828. Conn, C.E. et al. (2015). Convergent evolution of strigolactone perception enabled host detection in parasitic plants. Science 349: 540–543.

829. Yoshimoto, N. et al. (2015). Identification of a flavin-containing S-oxygenating monooxygenase involved in alliin biosynthesis in garlic. Plant J. 83: 941–951.

830. Lori, M. et al. (2015). Evolutionary divergence of the plant elicitor peptides (Peps) and their receptors: interfamily incompatibility of perception but compatibility of downstream signalling. J. Exp. Bot. 66: 5315–5325.

831. Plach, M.G., Löffler, P., Merkl, R., and Sterner, R. (2015). Conversion of anthranilate synthase into isochorismate synthase: implications for the evolution of chorismate-utilizing enzymes. Angew. Chem. Int. Ed. 54: 11270–11274.

832. Ng, J.L.P. et al. (2015). Flavonoids and auxin transport inhibitors rescue symbiotic nodulation in the Medicago truncatula cytokinin perception mutant cre1. Plant Cell 27: 2210–2226.

833. Frerot, E., Neirynck, N., Cayeux, I., Yuan, Y.H.-J., and Yuan, Y.-M. (2015). New umami amides: structure–taste relationship studies of cinnamic acid derived amides and the natural occurrence of an intense umami amide in Zanthoxylum piperitum. J. Agric. Food Chem. 63: 7161–7168.

834. Dadashipour, M., Ishida, Y., Yamamoto, K., and Asano, Y. (2015). Discovery and molecular and biocatalytic properties of hydroxynitrile lyase from an invasive millipede, Chamberlinius hualienensis. Proc. Natl. Acad. Sci. USA 112: 10605–10610.

835. Wang, H. et al. (2015). Rice WRKY4 acts as a transcriptional activator mediating defense responses toward Rhizoctonia solani, the causing agent of rice sheath blight. Plant Mol. Biol. 89: 157–171.

836. Zhang, F. et al. (2015). Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling. Nature 525: 269–273.

837. Ishiga, Y. et al. (2015). Transcriptomic and metabolomic analyses identify a role for chlorophyll catabolism and phytoalexin during Medicago nonhost resistance against Asian soybean rust. Sci. Rep. 5: 13061.

838. Lemarié, S. et al. (2015). Camalexin contributes to the partial resistance of Arabidopsis thaliana to the biotrophic soilborne protist Plasmodiophora brassicae. Front. Plant Sci. 6: 539.

839. Strugala, R., Delventhal, R., and Schaffrath, U. (2015). An organ-specific view on nonhost resistance. Front. Plant Sci. 6: 526.

840. Galanie, S., Thodey, K., Trenchard, I.J., Filsinger Interrante, M., and Smolke, C.D. (2015). Complete biosynthesis of opioids in yeast. Science 349: 1095–1100.

841. Patkar, R.N. et al. (2015). A fungal monooxygenase-derived jasmonate attenuates host innate immunity. Nat. Chem. Biol. 11: 733–740.

842. Speed, M.P., Fenton, A., Jones, M.G., Ruxton, G.D., and Brockhurst, M.A. (2015). Coevolution can explain defensive secondary metabolite diversity in plants. New Phytol. 208: 1251–1263.

843. Byeon, Y., Choi, G.-H., Lee, H.Y., and Back, K. (2015). Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice. J. Exp. Bot. 66: 6917–6925.

844. Clausen, M. et al. (2015). The bifurcation of the cyanogenic glucoside and glucosinolate biosynthetic pathways. Plant J. 84: 558–573.

845. Rodiuc, N. et al. Evolutionarily distant pathogens require the Arabidopsis phytosulfokine signalling pathway to establish disease. Plant Cell Environ. in press.

846. Kettle, A.J. et al. (2015). A γ-lactamase from cereal infecting Fusarium spp. catalyses the first step in the degradation of the benzoxazolinone class of phytoalexins. Fungal Genet. Biol. 83: 1–9.

847. Hidalgo, W., Chandran, J.N., Menezes, R.C., Otálvaro, F., and Schneider, B. Phenylphenalenones protect banana plants from infection by Mycosphaerella fijiensis and are deactivated by metabolic conversion. Plant Cell Environ. in press.

848. Moghe, G., and Last, R.L. (2015). Something old, something new: Conserved enzymes and the evolution of novelty in plant specialized metabolism. Plant Physiol. 169: 1512–1523.

849. Tsuchiya, Y. et al. (2015). Probing strigolactone receptors in Striga hermonthica with fluorescence. Science 349: 864–868.

850. Olate, V.R., Vélez, M.L., Greslebin, A., and Schmeda-Hirschmann, G. (2015). Phytophthora austrocedri elicitates changes in diterpene profile of Austrocedrus chilensis. Molecules 20: 15084–15097.

851. Falcone Ferreyra, M.L. et al. (2015). The identification of maize and Arabidopsis type I FLAVONE SYNTHASEs links flavones with hormones and biotic interactions. Plant Physiol. 169: 1090–1107.

852. Zheng, S. et al. (2015). Evaluating the effect of expressing a peanut resveratrol synthase gene in rice. PLoS ONE 10: e0136013.

853. Christensen, S.A. et al. (2015). Maize death acids, 9-lipoxygenase–derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators. Proc. Natl. Acad. Sci. USA 112: 11407–11412.

854. Ryffel, F. et al. Metabolic footprint of epiphytic bacteria on Arabidopsis thaliana leaves. ISME J. in press.

855. Zamioudis, C. et al. (2015). Rhizobacterial volatiles and photosynthesis-related signals coordinate MYB72 in Arabidopsis roots during onset of induced systemic resistance and iron deficiency responses. Plant J. 84: 309–322.

856. Hadwiger, L.A. (2015). Nonhost resistance: Self-inflicted DNA damage by fungal DNase accumulation is a major factor in terminating fungal growth in the pea-Fusarium solani f.sp. phaseoli interaction. Physiol. Mol. Plant Pathol. 92: 79–87.

857. Chamam, A., Wisniewski-Dyé, F., Comte, G., Bertrand, C., and Prigent-Combaret, C. (2015). Differential responses of Oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria. Planta 242: 1439–1452.

858. Rasher, D.B. et al. (2015). Marine and terrestrial herbivores display convergent chemical ecology despite 400 million years of independent evolution. Proc. Natl. Acad. Sci. USA 112: 12110–12115.

859. Ray, S. et al. (2015). Maize plants recognize herbivore-associated cues from caterpillar frass. J. Chem. Ecol. 41: 781–792.

860. Li, R. et al. (2015). Prioritizing plant defence over growth through WRKY regulation facilitates infestation by non-target herbivores. eLife 4: e04805.

861. Pedras, M.S.C., Alavi, M., and To, Q.H. (2015). Expanding the nasturlexin family: Nasturlexins C and D and their sulfoxides are phytoalexins of the crucifers Barbarea vulgaris and B. verna. Phytochemistry 118: 131–138.

862. Siciliano, I., Amaral Carneiro, G., Spadaro, D., Garibaldi, A., and Gullino, M. (2015). Jasmonic acid, abscisic acid and salicylic acid are involved in the phytoalexin responses of rice to Fusarium fujikuroi, a high gibberellin producer pathogen. J. Agric. Food Chem. 63: 8134–8142.

863. Xiao, G.-Q., Zhang, H.-W., Lu, X.-Y., and Huang, R.-F. (2015). Characterization and mapping of a novel light-dependent lesion mimic mutant lmm6 in rice (Oryza sativa L.). J. Integr. Agric. 14: 1687–1696.

864. Alamgir, K.M. et al. Systematic analysis of rice (Oryza sativa) metabolic responses to herbivory. Plant Cell Environ. in press.

865. Qi, J. et al. Jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice. J. Integr. Plant Biol. in press.

866. Hu, L. et al. (2015). The rice transcription factor WRKY53 suppresses herbivore-induced defenses by acting as a negative feedback modulator of mitogen-activated protein kinase activity. Plant Physiol. 169: 2907–2921.

867. Scholz, S.S., Reichelt, M., Boland, W., and Mithöfer, A. (2015). Additional evidence against jasmonate-induced jasmonate induction hypothesis. Plant Sci. 239: 9–14.

868. Poloni, A., and Schirawski, J. Host specificity in Sporisorium reilianum is determined by distinct mechanisms in maize and sorghum. Mol. Plant Pathol. in press.

869. Klein, A.P., and Sattely, E.S. (2015). Two cytochromes P450 catalyze S-heterocyclizations in cabbage phytoalexin biosynthesis. Nat. Chem. Biol. 11: 837–839.

870. Poreddy, S. et al. (2015). Detoxification of hostplant/’s chemical defence rather than its anti-predator co-option drives β-glucosidase-mediated lepidopteran counteradaptation. Nat. Commun. 6: 8525.

停電＆今週の輪読

明日の土曜日は毎年行われている電気設備点検のために農学部は朝から夕方まで停電になります．今日は停電に備えて MS や NMR をシャットダウンしました．

今週の輪読では下記の論文を取り上げました．

818. Wang, C. et al. The Arabidopsis Elongator subunit 2 positively contributes to resistance to the necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola. Plant J. in press.

819. Mo, H. et al. Cotton polyamine oxidase is required for spermine and camalexin signalling in the defence response to Verticillium dahliae. Plant J. in press.

820. Pfeilmeier, S., Saur, I.M.-L., Rathjen, J.P., Zipfel, C., and Malone, J.G. High levels of cyclic-di-GMP in plant-associated Pseudomonas correlate with evasion of plant immunity. Mol. Plant Pathol. in press.

821. Zwack, P.J., and Rashotte, A.M. (2015). Interactions between cytokinin signalling and abiotic stress responses. J. Exp. Bot. 66: 4863–4871.

822. Sripong, K. et al. (2015). Combined treatment with hot water and UV-C elicits disease resistance against anthracnose and improves the quality of harvested mangoes. Crop Prot. 77: 1–8.

先週の輪読

先週の輪読では下記の論文を取り上げました．

813. Fujisaki, K. et al. (2015). Rice Exo70 interacts with a fungal effector, AVR-Pii and is required for AVR-Pii-triggered immunity. Plant J. 83: 875–887.

814. Zhou, Z. et al. (2015). An Arabidopsis plasma membrane proton ATPase modulates JA signaling and is exploited by the Pseudomonas syringae effector protein AvrB for stomatal invasion. Plant Cell 27: 2032–2041.

815. Manosalva, P. et al. (2015). Conserved nematode signalling molecules elicit plant defenses and pathogen resistance. Nat. Commun. 6: 7795.

816. Benouaret, R., and Goupil, P. (2015). Grape marc extract-induced defense reactions and protection against Phytophthora parasitica are impaired in NahG tobacco plants. J. Agric. Food Chem. 63: 6653–6659.

817. Pruitt, R.N. et al. (2015). The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium. Sci. Adv. 1: e1500245.

全学一斉休業＆輪読

8/12-14 は茨城大学では全学一斉休業日になっていましたので，研究室も基本的にお休みでした．

8/10-20 の輪読では下記の論文を取り上げました．

807. Yahyaa, M. et al. Identification, functional characterization, and evolution of terpene synthases from a basal dicot. Plant Physiol. in press.

808. Ceja-Navarro, J.A. et al. (2015). Gut microbiota mediate caffeine detoxification in the primary insect pest of coffee. Nat. Commun. 6: 7618.

809. Joshi, J.R., Burdman, S., Lipsky, A., Yariv, S., and Yedidia, I. Plant phenolic acids affect the virulence of Pectobacterium aroidearum and P. carotovorum subsp brasiliense via quorum-sensing regulation. Mol. Plant Pathol. in press.

810. Widemann, E. et al. (2015). Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates. Phytochemistry 117: 388–399.

811. Lebeis, S.L. et al. (2015). Salicylic acid modulates colonization of the root microbiome by specific bacterial taxa. Science 349: 860–864.

812. Mou, S. et al. (2015). Over-expression of rice CBS domain containing protein, OsCBSX3, confers rice resistance to Magnaporthe oryzae inoculation. Int. J. Mol. Sci. 16: 15903–15917.

前学期終了＆今週の輪読

茨城大学は 8/7 で前期の期末試験が終了して夏休みに入りました．

今週の輪読では下記の論文を取り上げました．

802. Wang, Y. et al. Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice. J. Exp. Bot. in press.

803. Meharg, C., and Meharg, A.A. (2015). Silicon, the silver bullet for mitigating biotic and abiotic stress, and improving grain quality, in rice. Environ. Exp. Bot. 120: 8–17.

804. Zheng, X. et al. (2015). Spatial and temporal regulation of biosynthesis of the plant immune signal salicylic acid. Proc. Natl. Acad. Sci. USA 112: 9166–9173.

805. Cai, B.-D. et al. (2015). Profiling of phytohormones in rice under elevated cadmium concentration levels by magnetic solid-phase extraction coupled with liquid chromatography tandem mass spectrometry. J. Chromatogr. A 1406: 78–86.

806. Wang, Q. et al. (2015). (E)-β-caryophyllene functions as a host location signal for the rice white-backed planthopper Sogatella furcifera. Physiol. Mol. Plant Pathol. 91: 106–112.

農学部オープンキャンパス，夏休み自由研究相談，大学院入試，研究室セミナー，輪読

しばらくブログ更新をサボってしまいました．世間では夏休みに入っていますが，大学は今が期末試験期間で夏休みは来週からです．

7/18（土）には農学部でオープンキャンパスが開催されました．長谷川は別の業務で研究室見学には対応できなかったのですが，研究室の学生さんたちが高校生と保護者の方に熱心に説明してくれていたようです．

7/30（木）には茨城県教育委員会主催のいばらき理科教育推進事業で小学生の夏休み科学自由研究相談会が農学部で開催され，長谷川と生物制御化学研究室の大学院生 2 名が自由研究の進め方について助言を行いました．必ずしも専門に近くはない分野についての自由研究も多かったので，私も含め院生も事前の下調べが大変でしたが，勉強にはなりました．

8/1（土）には茨城大学大学院農学研究科修士課程の入学試験が実施されました．今年度から資源生物科学選考では英語の試験は外部試験（TOEIC など）のスコア提出に切り替わりましたので，当日の試験は専門科目と面接だけになりました．

7/17 の研究室セミナーでの文献紹介の担当は四年生の福木さんで，紹介した論文は Xu, S., Nguyen, T., Pomilio, I., Vitale, M.C., and Velu, S.E. (2014). Total synthesis of calothrixins A and B via oxidative radical reaction of cyclohexenone with aminophenanthridinedione. Tetrahedron 70: 5928–5933 でした．

7/24 の研究室セミナーでの文献紹介の担当は四年生の佐山君と化学生態学研究室修士二年生の藤原さんで，紹介した論文はそれぞれ Ditengou, F.A. et al. (2015). Volatile signalling by sesquiterpenes from ectomycorrhizal fungi reprogrammes root architecture. Nat. Commun. 6: 6279 と Qu, Y. et al. (2015). Completion of the seven-step pathway from tabersonine to the anticancer drug precursor vindoline and its assembly in yeast. Proc. Natl. Acad. Sci. USA 112: 6224–6229 でした．

7/13-7/30 の輪読では下記の論文を取り上げました．

790. Salas, R.A., Scott, R.C., Dayan, F.E., and Burgos, N.R. (2015). EPSPS gene amplification in glyphosate-resistant italian ryegrass (Lolium perenne ssp. multiflorum) populations from Arkansas (United States). J. Agric. Food Chem. 63: 5885–5893.

791. Cao, W.-L. et al. OsJAMyb, a R2R3-type MYB transcription factor, enhanced blast resistance in transgenic rice. Physiol. Mol. Plant Pathol. in press.

792. Chandler, S. et al. (2015). Role of cyclic lipopeptides produced by Bacillus subtilis in mounting induced immunity in rice (Oryza sativa L.). Physiol. Mol. Plant Pathol. 91: 20–30.

793. Lee, J.S. et al. (2015). Competitive binding of antagonistic peptides fine-tunes stomatal patterning. Nature 522: 439–443.

794. Wu, T. et al. (2015). 3,5-Diarylpyrazole derivatives obtained by ammonolysis of the total flavonoids from Chrysanthemum indicum extract show potential for the treatment of Alzheimer’s disease. J. Nat. Prod. 78: 1593–1599.

795. Winzer, T. et al. (2015). Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein. Science 349: 309–312.

796. Naseem, M., Kaltdorf, M., and Dandekar, T. (2015). The nexus between growth and defence signalling: auxin and cytokinin modulate plant immune response pathways. J. Exp. Bot. 66: 4885–4896.

797. Zerbe, P. et al. Exploring diterpene metabolism in non-model species: Transcriptome-enabled discovery and functional characterization of labda-7,13E-dienyl diphosphate synthase from Grindelia robusta. Plant J. in press.

798. Tamaki, H. et al. (2015). Targeting hormone-related pathways to improve grain yield in rice: a chemical approach. PLoS ONE 10: e0131213.

799. Müller, T.M. et al. (2015). TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE-mediated generation and metabolic analysis of camalexin-deficient cyp71a12 cyp71a13 double knockout lines. Plant Physiol. 168: 849–858.

800. Zhang, B., Xie, M., Bruschweiler-Li, L., Bingol, K., and Brüschweiler, R. (2015). Use of charged nanoparticles in NMR-based metabolomics for spectral simplification and improved metabolite identification. Anal. Chem. 87: 7211–7217.

801. Magnard, J.-L. et al. (2015). Biosynthesis of monoterpene scent compounds in roses. Science 349: 81–83.