先週は 12/25, 26 だけ輪読を実施しました．新年の輪読は 1/7 から開始です．
1427. Murphy, K.M., Ma, L.-T., Ding, Y., Schmelz, E.A., and Zerbe, P. (2018). Functional characterization of two class II diterpene synthases indicates additional specialized diterpenoid pathways in maize (Zea mays). Front. Plant Sci. 9: 1542.
1428. Elias, L.G. et al. (2018). A comparative venomic fingerprinting approach reveals that galling and non-galling fig wasp species have different venom profiles. PLOS ONE 13: e0207051.
今週の輪読では下記の論文を取り上げました．
1422. Huang, J. et al. (2019). Natural allelic variations provide insights into host adaptation of Phytophthora avirulence effector PsAvr3c. New Phytol. 221: 1010–1022.
1423. Chicca, A. et al. (2018). Uncovering the psychoactivity of a cannabinoid from liverworts associated with a legal high. Sci. Adv. 4: eaat2166.
1424. Castro, S.J. et al. (2018). Phytochemical study of Senecio volckmannii assisted by CASE-3D with residual dipolar couplings and isotropic 1H/13C NMR chemical shifts. J. Nat. Prod. 81: 2329–2337.
1425. Liu, Q. et al. (2018). OsWRKY67 positively regulates blast and bacteria blight resistance by direct activation of PR genes in rice. BMC Plant Biol. 18: 257.
1426. Crall, J.D. et al. (2018). Neonicotinoid exposure disrupts bumblebee nest behavior, social networks, and thermoregulation. Science 362: 683–686.
今週の研究室セミナーの文献紹介の担当は 4 年生の岡田君で，下記の論文を紹介しました．
今週の輪読では下記の論文を取り上げました．
1418. Huang, Q. et al. (2018). The Tryptophan decarboxylase 1 gene from Aegilops variabilis No.1 regulate the resistance against cereal cyst nematode by altering the downstream secondary metabolite contents rather than auxin synthesis. Front. Plant Sci. 9: 1297.
1419. Schlawis, C. et al. (2018). Structural elucidation of trace components combining GC/MS, GC/IR, DFT-calculation and synthesis–salinilactones, unprecedented bicyclic lactones from Salinispora bacteria. Angew. Chem. Int. Ed. 57: 14921–14925.
1420. García, T., Veloso, J., and Díaz, J. (2018). Vanillyl nonanoate induces systemic resistance and lignification in pepper plants. J. Plant Physiol. 231: 251–260.
1421. Uematsu, K., Kutsukake, M., and Fukatsu, T. (2018). Water-repellent plant surface structure induced by gall-forming insects for waste management. Biol. Lett. 14: 20180470.
11/30 の研究室セミナーの文献紹介の担当は 4 年生の木村君で，下記の論文を紹介しました．
12/7 の研究室セミナーの文献紹介の担当は化学生態学研究室 4 年生の菅野君で，下記の論文を紹介しました．
11/27 〜 12/6 の輪読では下記の論文を取り上げました．
1411. Fiorin, G.L. et al. (2018). Suppression of plant immunity by fungal chitinase-like effectors. Curr. Biol. 28: 3023–3030.e5.
1412. Toyota, M. et al. (2018). Glutamate triggers long-distance, calcium-based plant defense signaling. Science 361: 1112–1115.
1413. Wang, Y. et al. (2018). A MPK3/6-WRKY33-ALD1-pipecolic acid regulatory loop contributes to systemic acquired resistance. Plant Cell 30: 2480–2494.
1414. Kong, C.-H. et al. (2018). Plant neighbor detection and allelochemical response are driven by root-secreted signaling chemicals. Nat. Commun. 9: 3867.
1415. Cui, S., Wang, J., Gao, F., Sun, G., and Liang, J. (2018). Quantitative proteomic analyses reveal that RBBI3.3, a trypsin inhibitor protein, plays an important role in Magnaporthe oryzae infection in rice. Plant Growth Regul. 86: 365–374.
1416. Liu, Y., Cao, Y., Zhang, Q., Li, X., and Wang, S. (2018). A cytosolic triosephosphate isomerase is a key component in XA3/XA26-mediated resistance. Plant Physiol. 178: 923–935.
1417. Srivastava, A.K. et al. (2018). SUMO suppresses the activity of the jasmonic acid receptor CORONATINE INSENSITIVE1. Plant Cell 30: 2099–2115.
今週の研究室セミナーの文献紹介の担当は 4 年生の佐藤君で，下記の論文を紹介しました．
今週の輪読では下記の論文を取り上げました．
1408. Kwon, D.-H., Ji, J.-H., Yim, S.-H., Kim, B.-S., and Choi, H.-J. Suppression of influenza B virus replication by sakuranetin and mode of its action. Phytother. Res. in press.
1409. Mayer, D. et al. (2018). Short-term exposure to nitrogen dioxide provides basal pathogen resistance. Plant Physiol. 178: 468–487.
1410. Cagnola, J.I. et al. (2018). Long-day photoperiod enhances jasmonic acid-related plant defense. Plant Physiol. 178: 163–173.
今週の研究室セミナーの文献紹介の担当は 4 年生の早川君で，下記の論文を紹介しました．
今週の輪読では下記の論文を取り上げました．
1404. Perraki, A. et al. (2018). Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling. Nature 561: 248–252.
1405. Quareshy, M. et al. (2018). The tetrazole analogue of the auxin indole-3-acetic acid binds preferentially to TIR1 and not AFB5. ACS Chem. Biol. 13: 2585–2594.
1406. Wang, J. et al. (2018). A single transcription factor promotes both yield and immunity in rice. Science 361: 1026–1028.
1407. Takaoka, Y. et al. (2018). A rationally designed JAZ subtype-selective agonist of jasmonate perception. Nat. Commun. 9: 3654.
11/2 の研究室セミナーの文献紹介の担当は化学生態学研究室 4 年生の村上君で下記の論文を紹介しました．
11/9 の研究室セミナーの文献紹介の担当は化学生態学研究室 4 年生の古河君で下記の論文を紹介しました．
10/30〜11/8 の輪読では下記の論文を取り上げました．
1398. Chen, X.-L. et al. (2018). Proteomic analysis of ubiquitinated proteins in rice (Oryza sativa) after treatment with pathogen-associated molecular pattern (PAMP) elicitors. Front. Plant Sci. 9: 1064.
1399. Knox, K., Paterlini, A., Thomson, S., and Oparka, K.J. (2018). The coumarin glucoside, esculin, reveals rapid changes in phloem-transport velocity in response to environmental cues. Plant Physiol. 178: 795–807.
1400. Vicente, J. et al. Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytol. in press.
1401. Tian, D. et al. (2018). Proteomic analysis of the defense response to Magnaporthe oryzae in rice harboring the blast resistance gene Piz-t. Rice 11: 47.
1402. Liang, W. et al. (2018). A novel water-based chitosan-La pesticide nanocarrier enhancing defense responses in rice (Oryza sativa L) growth. Carbohydr. Polym. 199: 437–444.
1403. Bernardi, J. et al. (2018). Phenolic profile and susceptibility to Fusarium infection of pigmented maize cultivars. Front. Plant Sci. 9: 1189.
先週から研究室セミナーでの文献紹介が再開しました．
先週の担当は化学生態学研究室博士 3 年の武井さんで，下記の論文を紹介しました．
今週の担当は修士 2 年の大垣君で，下記の論文を紹介しました．
10/9〜26 の輪読では以下の論文を取り上げました．
1386. Li, W., Zhou, F., and Pichersky, E. (2018). Jasmone hydroxylase, a key enzyme in the synthesis of the alcohol moiety of pyrethrin insecticides. Plant Physiol. 177: 1498–1509.
1387. Takeda, Y. et al. (2018). Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification. Plant J. 95: 796–811.
1388. Toyomasu, T. et al. (2018). Characterization of diterpene synthase genes in the wild rice species Oryza brachyatha provides evolutionary insight into rice phytoalexin biosynthesis. Biochem. Biophys. Res. Commun. 503: 1221–1227.
1389. Kuroha, T. et al. (2018). Ethylene-gibberellin signaling underlies adaptation of rice to periodic flooding. Science 361: 181–186.
1390. Zhang, K.-L. et al. Herbivore-induced rice resistance against rice blast mediated by salicylic acid. Insect Sci. in press.
1391. Zhang, X. et al. (2018). A positive charged patch and stabilized hydrophobic core are essential for avirulence function of AvrPib in the rice blast fungus. Plant J. 96: 133–146.
1392. Pelot, K.A. et al. (2018). Pelot, K.A. et al. (2018). Functional diversity of diterpene synthases in the biofuel crop switchgrass. Plant Physiol. 178: 54–71.
1393. Salvador-Guirao, R. et al. OsDCL1a activation impairs phytoalexin biosynthesis and compromises disease resistance in rice. Ann. Bot. in press.
1394. Hu, L. et al. (2018). Root exudate metabolites drive plant-soil feedbacks on growth and defense by shaping the rhizosphere microbiota. Nat. Commun. 9: 2738.
1395. Pedras, M.S.C., and Abdoli, A. (2018). Methoxycamalexins and related compounds: syntheses, antifungal activity and inhibition of brassinin oxidase. Bioorg. Med. Chem. 26: 4461–4469.
1396. Gallego-Giraldo, L. et al. (2018). Elicitors and defense gene induction in plants with altered lignin compositions. New Phytol. 219: 1235–1251.
1397. Nishiguchi, S. et al. (2018). Accumulation of 9- and 13-KODEs in response to jasmonic acid treatment and pathogenic infection in rice. J. Pestic. Sci. 43: 191–197.
10 月になったので，今週から化学生態学研究室と合同の研究室セミナーが再開しました．今週と来週は中間発表会です．来年度卒論配属予定の 3 年生も参加しました．
今週の輪読は下記の論文を取り上げました．
1384. Yan, Y. et al. (2018). Resistance-gene-directed discovery of a natural-product herbicide with a new mode of action. Nature 559: 415–418.
1385. De la Concepcion, J.C. et al. (2018). Polymorphic residues in rice NLRs expand binding and response to effectors of the blast pathogen. Nat. Plants 4: 576–585.
茨城大学は 9/28（金）から後期の授業が始まりました．大学の後期というのは長い間 10/1 スタートだったのですが，最近は授業日程が非常にタイトになっていて，10/1 開始では間に合わないのです．そうなった理由は 15 回の授業と期末試験で 16 週を確保することが絶対になっていることと，クォーター制という一年を 4 分割したスケジュールでの授業も開講されているためです．
今週の輪読は下記の論文を取り上げました．
1384. Laura, B., Silvia, P., Francesca, F., Benedetta, S., and Carla, C. (2018). Epigenetic control of defense genes following MeJA-induced priming in rice (O. sativa). J. Plant Physiol. 228: 166–177.
1385. Boutanaev, A.M., and Osbourn, A.E. (2018). Multigenome analysis implicates miniature inverted-repeat transposable elements (MITEs) in metabolic diversification in eudicots. Proc. Natl. Acad. Sci. USA 115: E6650–E6658.
1386. Lin, S. et al. (2018). Quantitative proteomic analysis provides insights into rice defense mechanisms against Magnaporthe oryzae. Int. J. Mol. Sci. 19: 1950.
1387. Meng, Q. et al. A proteomic insight into the MSP1 and flg22 induced signaling in Oryza sativa leaves. J. Proteomics in press.
今週の輪読は下記の論文を取り上げました．
1382. Jeong, H. et al. (2018). Hepatic metabolism of sakuranetin and its modulating effects on cytochrome P450s and UDP-glucuronosyltransferases. Molecules 23: 1542.
1383. Fiorilli, V. et al. (2018). Omics approaches revealed how arbuscular mycorrhizal symbiosis enhances yield and resistance to leaf pathogen in wheat. Sci. Rep. 8: 9625.
今週の輪読は下記の論文を取り上げました．
1377. Hu, L. et al. (2018). OsLRR-RLK1, an early responsive leucine-rich repeat receptor-like kinase, initiates rice defense responses against a chewing herbivore. New Phytol. 219: 1079–1111.
1378. Persaud, R., and Saravanakumar, D. (2018). Differential expression of proteins in resistant and susceptible rice genotypes against blast infection. Physiol. Mol. Plant Pathol. 103: 62–70.
1379. Ge, Y. et al. Silencing of miR156 confers enhanced resistance to brown planthopper in rice. Planta in press.
1380. Ishimaru, Y. et al. (2018). Jasmonic acid inhibits auxin-induced lateral rooting independently of the CORONATINE INSENSITIVE 1 receptor. Plant Physiol. 177: 1704–1716.
1381. Suárez, I. et al. (2018). Structural and biosynthetic studies on eremophilenols related to the phytoalexin capsidiol, produced by Botrytis cinerea. Phytochemistry 154: 10–18.
今週の輪読は下記の論文を取り上げました．
1372. Mine, A. et al. (2018). The defense phytohormone signaling network enables rapid, high-amplitude transcriptional reprogramming during effector-triggered immunity. Plant Cell 30: 1199–1219.
1373. Ai, Y., and Zhu, Z. (2018). Melatonin antagonizes jasmonate-triggered anthocyanin biosynthesis in Arabidopsis thaliana. J. Agric. Food Chem. 66: 5392–5400.
1374. Chen, X. et al. (2018). A pathogenesis-related 10 protein catalyzes the final step in thebaine biosynthesis. Nat. Chem. Biol. 14: 738–743.
1375. Wang, Z. et al. (2018). Overexpression of OsPGIP2 confers Sclerotinia sclerotiorum resistance in Brassica napus through increased activation of defense mechanisms. J. Exp. Bot. 69: 3141–3155.
1376. Yoo, Y. et al. (2018). Lack of a cytoplasmic RLK, required for ROS homeostasis, induces strong resistance to bacterial leaf blight in rice. Front. Plant Sci. 9: 577.
今週の輪読は下記の論文を取り上げました．
1367. Xu, Y. et al. (2018). Leaf stage-associated resistance is correlated with phytohormones in a pathosystem-dependent manner. J. Integr. Plant Biol. 60: 703–722.
1368. Nakayasu, M. et al. (2018). JRE4 is a master transcriptional regulator of defense-related steroidal glycoalkaloids in tomato. Plant J. 94: 975–990.
1369. Cai, Q. et al. (2018). Plants send small RNAs in extracellular vesicles to fungal pathogen to silence virulence genes. Science 360: 1126–1129.
1370. Ohtaka, K., Hori, K., Kanno, Y., Seo, M., and Ohta, H. (2017). Primitive auxin response without TIR1 and Aux/IAA in the charophyte alga Klebsormidium nitens. Plant Physiol. 174: 1621–1632.
1371. Ngoc Minh, T. et al. (2018). Momilactones A and B: optimization of yields from isolation and purification. Separations 5: 28.
今週の輪読は下記の論文を取り上げました．
1362. Wang, K. et al. (2018). Two abscisic acid responsive plastid lipase genes involved in jasmonic acid biosynthesis in Arabidopsis thaliana. Plant Cell 30: 1006–1022.
1363. Grechkin, A.N. et al. (2018). Allene oxide synthase pathway in cereal roots: detection of novel oxylipin graminoxins. ChemistryOpen 7: 336–343.
1364. Wang, Z. et al. (2018). Osa-miR164a targets OsNAC60 and negatively regulates rice immunity against the blast fungus Magnaporthe oryzae. Plant J. 95: 584–597.
1365. Pupier, M. et al. (2018). NMReDATA, a standard to report the NMR assignment and parameters of organic compounds. Magn. Reson. Chem. 56: 703–715.
1366. Nagel, R., and Peters, R.J. (2018). Diverging mechanisms: cytochrome-p450-catalyzed demethylation and γ-lactone formation in bacterial gibberellin biosynthesis. Angew. Chem. Int. Ed. 57: 6082–6085.
8/4（土）は農学部オープンキャンパスが開催されました．非常に暑い日でしたが，800 名以上の来場者があったようで，生物制御化学研究室の研究室見学にも多くのお客さんが来て，四年生や大学院生の説明を聞いてくれました．
今週の研究室セミナーの文献紹介の担当は化学生態学研究室 4 年生の村上君で下記の論文を紹介しました．今週で夏休み前のセミナーは終了です．
今週の輪読は下記の論文を取り上げました．来週の輪読は休みです．
1357. Pedras, M.S.C.C., and To, H.Q. (2018). Interrogation of biosynthetic pathways of the cruciferous phytoalexins nasturlexins with isotopically labelled compounds. Org. Biomol. Chem. 16: 3625–3638.
1358. Tian, L. et al. (2018). Comparative analysis of the root transcriptomes of cultivated and wild rice varieties in response to Magnaporthe oryzae infection revealed both common and species-specific pathogen responses. Rice 11: 26.
1359. Lu, X. et al. (2018). Inferring roles in defense from metabolic allocation of rice diterpenoids. Plant Cell 30: 1119–1131.
1360. Zhou, P., Mo, X., Wang, W., Chen, X., and Lou, Y. (2018). The commonly used bactericide bismerthiazol promotes rice defenses against herbivores. Int. J. Mol. Sci. 19: 1271.
1361. Lu, H.-p. et al. (2018). Resistance of rice to insect pests mediated by suppression of serotonin biosynthesis. Nat. Plants 4: 338–344.
今週の研究室セミナーの文献紹介の担当は化学生態学研究室 4 年生の古河君で下記の論文を紹介しました．
今週の輪読は下記の論文を取り上げました．
1353. Ding, Y. et al. (2018). Opposite roles of salicylic acid receptors NPR1 and NPR3/NPR4 in transcriptional regulation of plant immunity. Cell 173: 1454–1467.
1354. Zhang, N. et al. (2018). Genome wide analysis of the transition to pathogenic lifestyles in Magnaporthales fungi. Sci. Rep. 8: 5862.
1355. Amruta, N. et al. (2018). Exploring the potentiality of novel rhizospheric bacterial strains against the rice blast fungus Magnaporthe oryzae. Plant Pathol. J. 34: 126–138.
1356. Chen, X. et al. The rice terpene synthase gene OsTPS19 functions as an (S)-limonene synthase in planta, and its overexpression leads to enhanced resistance to the blast fungus Magnaporthe oryzae. Plant Biotechnol. J. in press.
今週の研究室セミナーの文献紹介の担当は 4 年生の早川君で下記の論文を紹介しました．
今週の輪読は下記の論文を取り上げました．
1349. Monte, I. et al. (2018). Ligand-receptor co-evolution shaped the jasmonate pathway in land plants. Nat. Chem. Biol. 14: 480–488.
1350. Zhang, H. et al. (2018). A plant phytosulfokine peptide initiates auxin-dependent immunity through cytosolic Ca2+ signaling in tomato. Plant Cell 30: 652–667.
1351. Saffer, A.M., and Irish, V.F. (2018). Flavonol rhamnosylation indirectly modifies the cell wall defects of RHAMNOSE BIOSYNTHESIS1 mutants by altering rhamnose flux. Plant J. 94: 649–660.
1352. Singh, P.K. et al. (2018). Co-evolutionary interactions between host resistance and pathogen avirulence genes in rice-Magnaporthe oryzae pathosystem. Fungal Genet. Biol. 115: 9–19.
今週は月曜日が海の日で祝日だったため，研究室セミナーはありませんでした．今週の輪読は下記の論文を取り上げました．
1345. Shinya, T. et al. (2018). Integration of danger peptide signals with herbivore-associated molecular pattern signaling amplifies anti-herbivore defense responses in rice. Plant J. 94: 626–637.
1346. Fan, J. et al. (2018). The monocot-specific receptor-like kinase SDS2 controls cell death and immunity in rice. Cell Host Microbe 23: 498–510.
1347. Salvador-Guirao, R., Hsing, Y.-i., and San Segundo, B. (2018). The polycistronic miR166k-166h positively regulates rice immunity via post-transcriptional control of EIN2. Front. Plant Sci. 9: 337.
1348. Hu, Z. et al. (2018). Induction of systemic resistance in tomato against Botrytis cinerea by N-decanoyl-homoserine lactone via jasmonic acid signaling. Planta 247: 1217–1227.
今週の研究室セミナーの文献紹介の担当は 4 年生の佐藤君で下記の論文を紹介しました．
今週の輪読は下記の論文を取り上げました．
1340. Zhang, X. et al. (2018). Magnaporthe oryzae induces the expression of a microRNA to suppress the immune response in rice. Plant Physiol. 177: 352–368.
1341. Sakulkoo, W. et al. (2018). A single fungal MAP kinase controls plant cell-to-cell invasion by the rice blast fungus. Science 359: 1399–1403.
1342. Akogou, F.U.G., Besten, H.M.W.D., Kayodé, A.P.P., Fogliano, V., and Linnemann, A.R. (2018). Antimicrobial evaluation of red, phytoalexin-rich sorghum food biocolorant. PLOS ONE 13: e0194657.
1343. de Zélicourt, A. et al. (2018). Ethylene induced plant stress tolerance by Enterobacter sp. SA187 is mediated by 2-keto-methylthiobutyric acid production. PLOS Genetics 14: e1007273.
1344. Chini, A. et al. (2018). The fungal phytotoxin lasiojasmonate a activates the plant jasmonic acid pathway. J. Exp. Bot. 69: 3095–3102.
今週の研究室セミナーの文献紹介の担当は4 年生の木村君で下記の論文を紹介しました．
今週の輪読は下記の論文を取り上げました．
1337. Kang, H.R. et al. (2018). Chemical characterization of novel natural products from the roots of Asian rice (Oryza sativa) that control adipocyte and osteoblast differentiation. J. Agric. Food Chem. 66: 2677–2684.
1338. Kong, H.G., Shin, T.S., Kim, T.H., and Ryu, C.-M. (2018). Stereoisomers of the bacterial volatile compound 2,3-butanediol differently elicit systemic defense responses of pepper against multiple viruses in the field. Front. Plant Sci. 9: 90.
1339. Zhang, Q. et al. (2018). A new phenylpropane-pimarane heterodimer and a new ent-kaurene diterpene from the husks of Oryza sativa. Phytochem. Lett. 24: 120–124.
今週の研究室セミナーの文献紹介の担当は化学生態学研究室 4 年生の菅野君で下記の論文を紹介しました．
今週の輪読は下記の論文を取り上げました．
1335. Tariq, R. et al. (2018). Comparative transcriptome profiling of rice near-isogenic line carrying Xa23 under infection of Xanthomonas oryzae pv. oryzae. Int. J. Mol. Sci. 19: 717.
1336. Kiryu, M. et al. (2018). Rice terpene synthase 18 (OsTPS18) encodes a sesquiterpene synthase that produces an antibacterial (E)-nerolidol against a bacterial pathogen of rice. J. Gen. Plant Pathol. 84: 221–229.
今週の研究室セミナーの文献紹介の担当は 4 年生の岡田君で下記の論文を紹介しました．
今週の輪読は下記の論文を取り上げました．
1332. Robison, F.M. et al. Common bean varieties demonstrate differential physiological and metabolic responses to the pathogenic fungus Sclerotinia sclerotiorum. Plant Cell Environ. in press.
1333. Mafu, S. et al. (2018). Discovery, biosynthesis and stress-related accumulation of dolabradiene-derived defenses in maize. Plant Physiol. 176: 2677–2790.
1334. Filipe, O., De Vleesschauwer, D., Haeck, A., Demeestere, K., and Höfte, M. (2018). The energy sensor OsSnRK1a confers broad-spectrum disease resistance in rice. Sci. Rep. 8: 3864.
今週の研究室セミナーの文献紹介の担当は 4 年生の江里君で下記の論文を紹介しました．
今週からクォーター制の授業は第 2 クォーターに入ったので，基本的に毎日輪読を開催できるようになりました．今週の輪読は下記の論文を取り上げました．
1327. Kebede, A.Z., Johnston, A., Schneiderman, D., Bosnich, W., and Harris, L.J. (2018). Transcriptome profiling of two maize inbreds with distinct responses to Gibberella ear rot disease to identify candidate resistance genes. BMC Genomics 19: 131.
1328. Sathiyabama, M., and Manikandan, A. (2018). Application of copper-chitosan nanoparticles stimulate growth and induce resistance in finger millet (Eleusine coracana Gaertn.) plants against blast disease. J. Agric. Food Chem. 66: 1784–1790.
1329. Seguel, A. et al. (2018). PROHIBITIN 3 forms complexes with ISOCHORISMATE SYNTHASE 1 to regulate stress-induced salicylic acid biosynthesis in Arabidopsis. Plant Physiol. 176: 2515–2531.
1330. Buswell, W. et al. (2018). Chemical priming of immunity without costs to plant growth. New Phytol. 218: 1205–1216.
1331. Yimer, H.Z. et al. (2018). Gibberellin antagonizes jasmonate-induced defense against Meloidogyne graminicola in rice. New Phytol. 218: 646–660.
先週の研究室セミナーの文献紹介担当は修士 1 年の隠塚君で，下記の論文について紹介しました．
先週の輪読では下記の論文を取り上げました．
1324. Dong, S. et al. (2018). Identification of Magnaporthe oryzae-elicited rice novel miRNAs and their targets by miRNA and degradome sequencing. Eur. J. Plant Pathol. 151: 629–647.
1325. Morimoto, N. et al. (2018). Induced phenylamide accumulation in response to pathogen infection and hormone treatment in rice (Oryza sativa). Biosci. Biotechnol. Biochem. 82: 407–416.
1326. Engleder, M. et al. (2018). Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca. PLOS ONE 13: e0192653.
秋田県立大学秋田キャンパスで開催された日本農薬学会第 43 回大会で修士 1 年の隠塚君が「イネフィトアレキシンであるモミラクトン A のイネ病原真菌による代謝」のタイトルで口頭発表を行いました．農薬学会には 10 年以上参加していませんでしたが，生物制御化学研究室出身で現在秋田県立大学で教授をされている田母神繁先生が大会実行委員長ということもあり，久しぶりに参加しました．また，いつもは 3 月か 4 月の開催で日本農芸化学会大会の開催時期とも近く，発表ネタが用意しにくかったのですが，今年度は時期がずれた開催だったということもあります．
学会と関係ありませんが，秋田市内では街中で石油掘っていました．
今年度の研究室セミナーは 5/7 から開始で最初の 2 回は年間計画発表会でした．文献紹介は 5/21 から開始しました．
5/30 化学生態学研究室 D3 武井さん
McClerklin, S.A. et al. (2018). Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000. PLoS Pathog. 14: e1006811.
5/23〜6/1 の輪読では下記の論文を取り上げました．
1320. Yokotani, N. et al. (2018). OsWRKY24, a blast-disease responsive transcription factor, positively regulates rice disease resistance. J. Gen. Plant Pathol. 84: 85–91.
1321. Yoshimura, M. et al. (2018). Discovery of shoot branching regulator targeting strigolactone receptor DWARF14. ACS Cent. Sci. 4: 230–234.
1322. Chen, H. et al. (2018). Effects of overexpression of jasmonic acid biosynthesis genes on nicotine accumulation in tobacco. Plant Direct 2: e00036.
1323. Hu, W., Pan, X., Li, F., and Dong, W. (2018). UPLC-QTOF-MS metabolomics analysis revealed the contributions of metabolites to the pathogenesis of Rhizoctonia solani strain AG-1-IA. PLOS ONE 13: e0192486.
今週は都合により輪読は 2 回しかできませんでした．
1318. Guo, J. et al. (2018). Bph6 encodes an exocyst-localized protein and confers broad resistance to planthoppers in rice. Nat. Genet. 50: 297–306.
1319. Liang, Y. et al. (2018). Infection with blast fungus (Magnaporthe orzyae) leads to increased expression of an arabinogalactan-protein epitope in both susceptible and resistant rice cultivars. Physiol. Mol. Plant Pathol. 102: 136–143.
しばらくブログ更新をサボりました．この間 4/23〜5/10 の輪読では下記の論文を取り上げました．第 1 クォーターは私が金曜日に水戸で授業があり，ゼミが月曜日 3 講時に決まってしまった（教員・学生の授業等の関係であまり洗濯の余地がない）ので，ちょっと輪読のできる回数が少なくなっています．
1309. Tripathi, D., Zhang, T., Koo, A.J., Stacey, G., and Tanaka, K. (2018). Extracellular ATP acts on jasmonate signaling to reinforce plant defense. Plant Physiol. 176: 511–523.
1310. Zhu, X. et al. (2016). The multivesicular bodies (MVBs)-localized AAA ATPase LRD6-6 inhibits immunity and cell death likely through regulating MVBs-mediated vesicular trafficking in rice. PLOS Genetics 12: e1006311.
1311. He, S.-L., Jiang, J.-Z., Chen, B.-H., Kuo, C.-H., and Ho, S.-L. (2018). Overexpression of a constitutively active truncated form of OsCDPK1 confers disease resistance by affecting OsPR10a expression in rice. Sci. Rep. 8: 403.
1312. Liu, N. et al. (2018). A pectin methylesterase inhibitor enhances resistance to Verticillium wilt. Plant Physiol. 176: 2202–2220.
1313. Bao, Z., Clancy, M.A., Carvalho, R.F., Elliott, K., and Folta, K.M. (2017). Identification of novel growth regulators in plant populations expressing random peptides. Plant Physiol. 175: 619.
1314. Waqas, M. et al. (2018). Biochar amendment changes jasmonic acid levels in two rice varieties and alters their resistance to herbivory. PLOS ONE 13: e0191296.
1315. Christensen, S.A. et al. (2018). Commercial hybrids and mutant genotypes reveal complex protective roles for inducible terpenoid defenses. J. Exp. Bot. 69: 1693–1705.
1316. Vo, K.T.X. et al. (2018). OsWRKY67 plays a positive role in basal and XA21-mediated resistance in rice. Front. Plant Sci. 8: 2220.
1317. Shi, X. et al. (2018). The fungal pathogen Magnaporthe oryzae suppresses innate immunity by modulating a host potassium channel. PLoS Pathog. 14: e1006878.
今週の輪読では下記の論文を取り上げました．
1305. Hu, L. et al. (2017). The coiled-coil and nucleotide binding domains of BROWN PLANTHOPPER RESISTANCE14 function in signaling and resistance against planthopper in rice. Plant Cell 29: 3157–3185.
1306. Piślewska-Bednarek, M. et al. (2018). Glutathione transferase U13 functions in pathogen-triggered glucosinolate metabolism. Plant Physiol. 176: 538–551.
1307. Shangguan, X. et al. (2018). A mucin-like protein of planthopper is required for feeding and induces immunity response in plants. Plant Physiol. 176: 552–565.
1308. Xu, H. et al. (2018). Coexpression analysis identifies two oxidoreductases involved in the biosynthesis of the monoterpene acid moiety of natural pyrethrin insecticides in Tanacetum cinerariifolium. Plant Physiol. 176: 524–537.
茨城大学は 4/10 から今年度の授業が始まりました．農学部では，新しい学科の食生命科学科と地域総合農学科の 2 年生が阿見キャンパスで授業を受けるようになったため，時間割など色々大きな変更がありました．また，履修登録，成績管理，e-ラーニングなどに使うシステムが今年度から新しいシステムに切り替わったのですが，稼働当初ということもあり，色々な不具合が出ていて，ちょっと混乱しています．
今週の輪読では下記の論文を取り上げました．
1301. Xu, M. et al. The feruloyl esterase genes are required for full pathogenicity of the apple tree canker pathogen Valsa mali. Mol. Plant Pathol. in press.
1302. McClerklin, S.A. et al. (2018). Indole-3-acetaldehyde dehydrogenase-dependent auxin synthesis contributes to virulence of Pseudomonas syringae strain DC3000. PLoS Pathog. 14: e1006811.
1303. Ma, J. et al. (2018). Disruption of OsSEC3A increases the content of salicylic acid and induces plant defense responses in rice. J. Exp. Bot. 69: 1051–1064.
1304. Reveglia, P. et al. (2018). Synthesis and mode of action studies of N-[(-)-jasmonyl]-S-tyrosin and ester seiridin jasmonate. Phytochemistry 147: 132–139.
新年度が始まりましたので，新メンバーでの輪読開始です．．今週の輪読では下記の論文を取り上げました．
1298. Fu, J. et al. (2018). ZmWRKY79 positively regulates maize phytoalexin biosynthetic gene expression and is involved in stress response. J. Exp. Bot. 69: 497–510.
1299. Tayi, L. et al. A mutation in an exoglucanase of Xanthomonas oryzae pv. oryzae, which confers an endo mode of activity, affects bacterial virulence, but not the induction of immune responses, in rice. Mol. Plant Pathol. in press.
1300. Chini, A. et al. (2018). An OPR3-independent pathway uses 4,5-didehydrojasmonate for jasmonate synthesis. Nat. Chem. Biol. 14: 171–178.
今日から新年度が始まりました．生物制御化学研究室は昨年度で修士 2 名が修了，四年生 3 名が卒業（うち 1 名が大学院進学）しました．今年度は新四年生 5 名が入って来たので，学生数は 1 名増えて，修士 2 名，四年生 5 名の体制になりました．
教員は戸嶋先生が今年度から農学部長です（任期 2 年）．長谷川は教授に昇進し，食生命科学科長となりました（これも任期 2 年）．
3/15 〜 18 に名古屋の名城大学天白キャンパスなどで開催された日本農芸化学会 2018 年度大会に生物制御化学研究室からは 1 題の研究発表をしました．
3 月 16 日（金）
2A18a08 イネ紋枯病菌によるフィトアレキシン代謝産物の構造決定及び抗菌活性
○勝間田駿，隠塚修平，戸嶋浩明，長谷川守文（茨城大農）
修士 2 年の勝間田君の研究室では最後の学会発表となりましたが，分かりやすく発表して，質問への受け答えもしっかりできていたと思います．
輪読は今年度は先週で終了です．先週の輪読では下記の論文を取り上げました．
1295. Christensen, S.A. et al. (2018). Fungal and herbivore elicitation of the novel maize sesquiterpenoid, zealexin A4, is attenuated by elevated CO2. Planta 247: 863–873.
1296. Wang, Y. et al. (2018). Proteomic analyses of Magnaporthe oryzae development disrupted by salicylic acid. Physiol. Mol. Plant Pathol. 102: 55–66.
1297. Blüher, D. et al. (2017). A 1-phytase type III effector interferes with plant hormone signaling. Nat. Commun. 8: 2159.
今週の輪読では下記の論文を取り上げました．
1292. Wang, J. et al. (2017). The kinase OsCPK4 regulates a buffering mechanism that fine-tunes innate immunity. Plant Physiol. 176: 1835–1849.
1293. Peng, L. et al. (2017). Functional study of cytochrome P450 enzymes from the brown planthopper (Nilaparvata lugens Stål) to analyze its adaptation to BPH-resistant rice. Frontiers in Physiology 8: 972.
1294. Patharkar, O.R., Gassmann, W., and Walker, J.C. (2017). Leaf shedding as an anti-bacterial defense in Arabidopsis cauline leaves. PLOS Genetics 13: e1007132.
先週の輪読では下記の論文を取り上げました．
1288. Malinovsky, F.G. et al. (2017). An evolutionary young defense metabolite influences the root growth of plants via the ancient TOR signaling pathway. eLife 6: e29353.
1289. Farag, A.M., Westphal, H., Eissa, F.T., Wessjohann, A.L., and Meyer, A. (2017). Effect of oxylipins, terpenoid precursors and wounding on soft corals’ secondary metabolism as analyzed via UPLC/MS and chemometrics. Molecules 22: 2195.
1290. Zhang, Q. et al. Increasing rice allelopathy by induction of barnyard grass (Echinochloa crus-galli) root exudates. J. Plant Growth Regul. in press.
1291. Lenarčič, T. et al. (2017). Eudicot plant-specific sphingolipids determine host selectivity of microbial NLP cytolysins. Science 358: 1431–1434.
2/19, 20 には資源生物科学科卒業論文発表会が開催され，全体では 40 名の学生の発表があり，生物制御化学研究室からは 3 名の四年生が発表をしました．
先週の輪読では下記の論文を取り上げました．
1285. Mélida, H. et al. (2018). Non-branched β-1,3-glucan oligosaccharides trigger immune responses in Arabidopsis. Plant J. 93: 34–49.
1286. Ullah, C. et al. (2017). Flavan-3-ols are an effective chemical defense against rust infection. Plant Physiol. 175: 1560–1578.
1287. Sobolev, V.S. et al. (2018). Suppression of aflatoxin production in Aspergillus species by selected peanut (Arachis hypogaea) stilbenoids. J. Agric. Food Chem. 66: 118–126.
2/14 には資源生物科学専攻の修士論文発表会が開催されました．生物制御化学研究室からは下記 2 名の学生が発表しました．農学研究科は今年度の一年生から専攻が改組されて農学専攻の一専攻体制に変わったので，10 月入学生などまだ少数の学生は残っていますが，資源生物科学専攻としては今回が概ね最後の修士論文発表会となりました．
北村遼「植物ホルモン様活性を指向した環状構造導入型KODA誘導体の合成研究」
勝間田駿「イネの病原菌によるフィトアレキシンの解毒代謝に関する研究」
研究室の学生は 2 名とも堂々と発表できていたと思います．
今週の輪読では下記の論文を取り上げました．
1283. Desaki, Y. et al. (2018). OsCERK1 plays a crucial role in the lipopolysaccharide-induced immune response of rice. New Phytol. 217: 1042–1049.
1284. Zhang, X.-B. et al. (2018). A substitution mutation in OsPELOTA confers bacterial blight resistance by activating the salicylic acid pathway. J. Integr. Plant Biol. 60: 160–172.
今週の輪読では下記の論文を取り上げました．
1283. Rais, A., Jabeen, Z., Shair, F., Hafeez, F.Y., and Hassan, M.N. (2017). Bacillus spp., a bio-control agent enhances the activity of antioxidant defense enzymes in rice against Pyricularia oryzae. PLOS ONE 12: e0187412.
1284. Regente, M. et al. (2017). Plant extracellular vesicles are incorporated by a fungal pathogen and inhibit its growth. J. Exp. Bot. 68: 5485–5495.
1285. Betsuyaku, S. et al. (2018). Salicylic acid and jasmonic acid pathways are activated in spatially different domains around the infection site during effector-triggered immunity in Arabidopsis thaliana. Plant Cell Physiol. 59: 8–16.
1286. Bastías, D.A. et al. (2018). The plant hormone salicylic acid interacts with the mechanism of anti-herbivory conferred by fungal endophytes in grasses. Plant Cell Environ. 41: 395–405.
1287. Tripathi, D., Zhang, T., Koo, A.J.K., Stacey, G., and Tanaka, K. (2017). Extracellular ATP acts on jasmonate signaling to reinforce plant defense. Plant Physiol. 176: 511–523.
1/31 は卒業論文発表会の要旨の締め切り，2/1 は修士論文の締め切りでした．修士論文発表会は 2/14，卒業論文発表会は 2/19, 20 の予定で，いよいよ残り時間が少なくなってラストスパートの時期になってきました．
今週の輪読では下記の論文を取り上げました．
1279. Meyer, J., Berger, D.K., Christensen, S.A., and Murray, S.L. (2017). RNA-Seq analysis of resistant and susceptible sub-tropical maize lines reveals a role for kauralexins in resistance to grey leaf spot disease, caused by Cercospora zeina. BMC Plant Biol. 17: 197.
1280. Liñeiro, E. et al. Phenotypic effects and inhibition of botrydial biosynthesis induced by different plant-based elicitors in Botrytis cinerea. Curr. Microbiol. in press.
1281. Ye, Z. et al. In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2. Biosci. Biotechnol. Biochem. in press.
1282. Yang, T. et al. (2018). Stilbenoid prenyltransferases define key steps in the diversification of peanut phytoalexins. J. Biol. Chem. 293: 28–46.
修士課程 2 年に在学中の勝間田駿君の修士論文研究の一部をまとめた原著論文がスイス MDPI 社のオープンアクセスの英文学術雑誌 Molecules に掲載されました．
イネ紋枯病菌はいもち病菌と並んでイネに大きな被害を及ぼす病原真菌です．この論文では，紋枯病菌がイネのフラボノイド系フィトアレキシンであるサクラネチンを解毒代謝する能力を有しており，その解毒代謝産物 3 種類を同定したことを報告しています．いもち病菌によるサクラネチンの解毒については，すでに勝間田君が報告しています．紋枯病菌によるサクラネチンの解毒代謝産物では，いもち病菌の解毒代謝産物としても報告されていたナリンゲニンも同定されましたが，それ以外に従来のフィトアレキシン解毒代謝では知られていなかったキシロシル化による解毒代謝産物であるサクラネチン-4′-O-β-D-キシロピラノシド，ナリンゲニン-7-O-β-D-キシロピラノシドを新たに同定することができました．これらの解毒代謝産物の抗菌活性はサクラネチンと比較して顕著に低く，サクラネチンを添加して培養すると，サクラネチンの現象に伴ってこれらの化合物が蓄積することが明らかとなりました．一方で，いもち病菌の解毒代謝産物として同定されたステルンビンへの変換は紋枯病菌ではみられませんでした．この論文の結果から，いもち病菌と紋枯病菌はそれぞれ独自にサクラネチンの解毒代謝能を獲得してきたのではないかと考えられます．
修士課程在学中に 2 報の筆頭著者論文が掲載された学生は私の指導学生では勝間田君が初めてです．原著論文は出ましたが，修士論文は明日 2/1 が締め切りですので，今が最後の追い込みというところです．
Molecules はオープンアクセスジャーナルなので，掲載料が 1800 CHF（約 20 万円）と非常に高価です．しかし，Molecules を含む MDPI（他には Int. J. Mol. Sci. などが有名）からの査読を引き受けて期限（一週間）以内に査読レポートを返すと，掲載料に使える 250 CHF のバウチャーがもらえるという仕組みになっています．MDPI からの査読依頼をかなりせっせと引き受けたので，今回のこの論文は掲載料を全額バウチャーで賄うことができ，実際には無料で掲載してもらえました．査読は労力のわりにリターンの少ない仕事なのですが，この仕組みは査読の引き受け甲斐を上げてくれる良いやり方だと思います．MDPI の査読バウチャーも以前は 50 CHF でしかも有効期限が 1 年だけだったのですが，現在は 250 CHF で有効期限 2 年になったので，かなり使えるようになりました．
今週の輪読では下記の論文を取り上げました．
1275. Andika, I.B. et al. (2017). Phytopathogenic fungus hosts a plant virus: A naturally occurring cross-kingdom viral infection. Proc. Natl. Acad. Sci. USA 114: 12267–12272.
1276. Xie, S. et al. Bacillus amyloliquefaciens FZB42 repressed plant miR846 to induce systemic resistance via jasmonic acid-dependent signaling pathway. Mol. Plant Pathol. in press.
1277. Kamatham, S., Pallu, R., Pasupulati, A.K., Singh, S.S., and Gudipalli, P. (2017). Benzoylsalicylic acid derivatives as defense activators in tobacco and Arabidopsis. Phytochemistry 143: 160–169.
1278. Li, X. et al. (2018). Effects of UV-B radiation on the infectivity of Magnaporthe oryzae and rice disease-resistant physiology in Yuanyang terraces. Photochem. Photobiol. Sci. 17: 8–17.
水曜日の研究室セミナーの文献紹介の担当は化学生態学研究室の小暮君で下記の論文を紹介しました．
今週で研究室セミナーは終了で，2/1 の修論提出やその後の修論・卒論発表会に向けて研究室が最後の追い込み期間に入っていきます．
今週の輪読では下記の論文を取り上げました．
1271. Khanam, S. et al. Mechanisms of resistance in the rice cultivar Manikpukha to the rice stem nematode Ditylenchus angustus. Mol. Plant Pathol. in press.
1272. Guo, L. et al. (2017). Echinochloa crus-galli genome analysis provides insight into its adaptation and invasiveness as a weed. Nat. Commun. 8: 1031.
1273. Shah, S.J. et al. (2017). Damage-associated responses of the host contribute to defence against cyst nematodes but not root-knot nematodes. J. Exp. Bot. 68: 5949–5960.
1274. Ding, Y. et al. (2017). Selinene volatiles are essential precursors for maize defense promoting fungal pathogen resistance. Plant Physiol. 175: 1455–1468.
今週末は茨城大学農学部でもセンター試験が実施されるので，準備のために金曜日の授業は休講でした．
今週の輪読では下記の論文を取り上げました．
1268. Rizza, A., Walia, A., Lanquar, V., Frommer, W.B., and Jones, A.M. (2017). In vivo gibberellin gradients visualized in rapidly elongating tissues. Nat. Plants 3: 803–813.
1269. Kloth, K.J. et al. (2017). SIEVE ELEMENT-LINING CHAPERONE 1 restricts aphid feeding on Arabidopsis during heat stress. Plant Cell 29: 2450–2464.
1270. Goupil, P., Benouaret, R., and Richard, C. (2017). Ethyl gallate displays elicitor activities in tobacco plants. J. Agric. Food Chem. 65: 9006–9012.
ブログ更新をサボっていたので，昨年 10 月以降の研究室セミナーで紹介された論文をまとめて紹介します．
10/26 武井（化学生態学研究室）
Zhang, Y. et al. (2017). S5H/DMR6 encodes a salicylic acid 5-hydroxylase that fine-tunes salicylic acid homeostasis. Plant Physiol. 175: 1082–1093.
2018 年が始まりました．研究室は 1/4 から仕事始めで，修論・卒論の追い込み時期になっています．12/25 〜 1/5 の輪読では下記の論文を取り上げました．
1264. Sun, T. et al. (2018). TGACG-BINDING FACTOR 1 (TGA1) and TGA4 regulate salicylic acid and pipecolic acid biosynthesis by modulating the expression of SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) and CALMODULIN-BINDING PROTEIN 60g (CBP60g). New Phytol. 217: 344–354.
1265. Chitarrini, G. et al. (2017). Identification of biomarkers for defense response to Plasmopara viticola in a resistant grape variety. Front. Plant Sci. 8: 1524.
1266. Qi, Z. et al. (2017). Enhanced resistance to rice blast and sheath blight in rice (Oryza sativa L.) by expressing the oxalate decarboxylase protein Bacisubin from Bacillus subtilis. Plant Sci. 265: 51–60.
1267. Nagel, R., and Peters, R.J. (2017). 18O2 labeling experiments illuminate the oxidation of ent-kaurene in bacterial gibberellin biosynthesis. Org. Biomol. Chem. 15: 7566–7571.
