今週の輪読

今週の輪読では下記の論文を取り上げました．
606. Meesters, C. et al. A chemical inhibitor of jasmonate signaling targets JAR1 in Arabidopsis thaliana. Nat. Chem. Biol. in press.
607. Lee, S.-H. et al. Mutation of Oryza sativa CORONATINE INSENSITIVE 1b (OsCOI1b) delays leaf senescence. J. Integr. Plant Biol. in press.
608. Miyamoto, K. et al. (2014). Identification of target genes of the bZIP transcription factor OsTGAP1, whose overexpression causes elicitor-induced hyperaccumulation of diterpenoid phytoalexins in rice cells. PLoS ONE 9: e105823.
609. King, A.J., Brown, G.D., Gilday, A.D., Larson, T.R., and Graham, I.A. Production of bioactive diterpenoids in the Euphorbiaceae depends on evolutionarily conserved gene clusters. Plant Cell in press.
610. Gong, B. et al. (2014). Nitric oxide, as a downstream signal, plays vital role in auxin induced cucumber tolerance to sodic alkaline stress. Plant Physiol. Biochem. 83: 258–266.

停電 & 先週の輪読

9/6（土）は年に一度の電気設備点検のための停電がありました．停電に備えて，普段は常時稼働している NMR, LC/MS/MS, GC/MS のシャットダウンを行い，ついでに LC/MS/MS はロータリーポンプのオイル交換やインターフェース部の洗浄といったメンテナンスも実施しました．

先週の輪読では下記の論文を取り上げました．
601. Fernandez, J. et al. Plant defense suppression is mediated by a fungal sirtuin during rice infection by Magnaporthe oryzae. Mol. Microbiol. in press.
602. Kouzai, Y. et al. (2014). Targeted gene disruption of OsCERK1 reveals its indispensable role in chitin perception and involvement in the peptidoglycan response and immunity in rice. Mol. Plant Microbe Interact. 27: 975–982.
603. Singh, P., and Shah, K. (2014). Evidences for reduced metal-uptake and membrane injury upon application of nitric oxide donor in cadmium stressed rice seedlings. Plant Physiol. Biochem. 83: 180–184.
604. Leone, M., Keller, M.M., Cerrudo, I., and Ballaré, C.L. To grow or defend? Low red : far-red ratios reduce jasmonate sensitivity in Arabidopsis seedlings by promoting DELLA degradation and increasing JAZ10 stability. New Phytol. in press.
605. Christeller, J.T., and Galis, I. (2014). α-Linolenic acid concentration and not wounding per se is the key regulator of octadecanoid (oxylipin) pathway activity in rice (Oryza sativa L.) leaves. Plant Physiol. Biochem. 83: 117–125.

水漏れ & 今週の輪読

8/29 に生物制御化学第二研究室の実験台の中を通っている水道管の腐食による破損が発生し，研究室が水浸しになるという事故が発生しました．大学の施設管理にお願いして水道の元栓を閉めてもらったのですが，事故発生時に担当の人が席を外されていたため，元栓を締めるまでにしばらく時間がかかってしまいました．その間にも水はどんどんあふれてきて，二研だけでなく一研，戸嶋先生の教授室，私の部屋，測定室，廊下がかなりの水浸しになってしまいました．学生さんたちと総出で排水作業を行って，三時間ぐらいで何とか復旧することができましたが，かなり大変な作業でした．すぐに水道屋さんが来てくれて，応急処置として破損した水道管を閉じてくれたので，他の場所では水道も使えるようになりました．

大学の研究室が入っている建物も築 20 年以上が経過しているため，いろいろガタが来ているようです．水道管も一斉点検や交換などの保守をした方が良い時期だと思うのですが，なかなか予算が取れないようで，簡単にはいかないみたいです．しかし，このような事故が休日や夜間，あるいは上の階（我々の研究室は一階）で起こっていたら，もっと大変なことになっていたはずなので，今後もちょっと心配です．

今週の輪読では下記の論文を取り上げました．
597. Agarrwal, R., Bentur, J.S., and Nair, S. Gas chromatography mass spectrometry based metabolic profiling reveals biomarkers involved in rice-gall midge interactions. J. Integr. Plant Biol. in press.
598. Bosch, M. et al. Jasmonic acid and its precursor 12-oxophytodienoic acid control different aspects of constitutive and induced herbivore defenses in tomato. Plant Physiol. in press.
599. Zhang, H., Wu, Z., Wang, C., Li, Y., and Xu, J.-R. (2014). Germination and infectivity of microconidia in the rice blast fungus Magnaporthe oryzae. Nat. Commun. 5: 4518.
600. Hasegawa, M. et al. (2014). Analysis on blast fungus-responsive characters of a flavonoid phytoalexin sakuranetin; accumulation in infected rice leaves, antifungal activity and detoxification by fungus. Molecules 19: 11404–11418.

大学院入試，夏休み，輪読

またしばらくブログ更新をさぼってしまいました． 8/2 には茨城大学大学院農学研究科の入試が実施されました．ここ二三年，大学院進学者が減少傾向ですので，なるべく多くの受験者に合格していただきたいところです．

茨城大学では 8/6 で前期の期末試験が終了して，三年生以下は夏休みに入りました．研究室は 8/13-17 は大学で決まっている一斉休業期間だったので，お休みにしましたが，それ以外は通常営業です．

7/28-8/22 の輪読では夏期の論文を取り上げました．
581. Yu, P., Hegeman, A.D., and Cohen, J.D. A facile means for the identification of indolic compounds from plant tissues. Plant J. in press.
582. Zhang, Y., Smith, P., Maximova, S.N., and Guiltinan, M.J. Application of glycerol as a foliar spray activates the defence response and enhances disease resistance of Theobroma cacao. Mol. Plant Pathol. in press.
583. Nguyen, V.N.T., Moon, S., and Jung, K.-H. (2014). Genome-wide expression analysis of rice ABC transporter family across spatio-temporal samples and in response to abiotic stresses. J. Plant Physiol. 171: 1276–1288.
584. Shiga, K. et al. (2014). Metabolic profiling approach to explore compounds related to the umami intensity of soy sauce. J. Agric. Food Chem. 62: 7317–7322.
585. Li, R.-Y., Wu, X.-M., Yin, X.-H., Liang, J.-N., and Li, M. (2014). The natural product citral can cause significant damage to the hyphal cell walls of Magnaporthe grisea. Molecules 19: 10279–10290.
586. Xie, G., Sasaki, K., Imai, R., and Xie, D. (2014). A redox-sensitive cysteine residue regulates the kinase activities of OsMPK3 and OsMPK6 in vitro. Plant Sci. 227: 69–75.
587. Huynh, N.T., Smagghe, G., Gonzales, G.B., Van, C., John, and Raes, K. (2014). Enzyme-assisted extraction enhancing the phenolic release from cauliflower (Brassica oleracea L. var. botrytis) outer leaves. J. Agric. Food Chem. 62: 7468–7476.
588. Awakawa, T. et al. (2014). A methyltransferase initiates terpene cyclization in teleocidin B biosynthesis. J. Am. Chem. Soc. 136: 9910–9913.
589. Petutschnig, E.K. et al. A novel Arabidopsis CHITIN ELICITOR RECEPTOR KINASE 1 (CERK1) mutant with enhanced pathogen-induced cell death and altered receptor processing. New Phytol. in press.
590. Akagi, A. et al. WRKY45-dependent priming of diterpenoid phytoalexin biosynthesis in rice and the role of cytokinin in triggering the reaction. Plant Mol. Biol. in press.
591. Becker, L., Carré, V., Poutaraud, A., Merdinoglu, D., and Chaimbault, P. (2014). MALDI mass spectrometry imaging for the simultaneous location of resveratrol, pterostilbene and viniferins on grapevine leaves. Molecules 19: 10587–10600.
592. Xu, X.-H. et al. (2014). The rice endophyte Harpophora oryzae genome reveals evolution from a pathogen to a mutualistic endophyte. Sci. Rep. 4: 5783.
593. Chen, M. et al. A novel elicitor identified from Magnaporthe oryzae triggers defense responses in tobacco and rice. Plant Cell Rep. in press.
594. Schenk, S.T. et al. (2014). N-Acyl-homoserine lactone primes plants for cell wall reinforcement and induces resistance to bacterial pathogens via the salicylic acid/oxylipin pathway. Plant Cell 26: 2708–2723.
595. Kazan, K., and Lyons, R. (2014). Intervention of phytohormone pathways by pathogen effectors. Plant Cell 26: 2285–2309.
596. Hayashi, K.- et al. (2014). Auxin transport sites are visualized in planta using fluorescent auxin analogs. Proc. Natl. Acad. Sci. USA 111: 11557–11562.

今週の輪読

今週の輪読では下記の論文を取り上げました．
577. Hallmann, C.A., Foppen, R.P.B., van, T., Chris A. M., de, K., Hans, and Jongejans, E. (2014). Declines in insectivorous birds are associated with high neonicotinoid concentrations. Nature 511: 341–343.
578. Yang, J.-O., Nakayama, N., Toda, K., Tebayashi, S., and Kim, C.-S. (2014). Structural determination of elicitors in Sogatella furcifera (Horváth) that induce Japonica rice plant varieties (Oryza sativa L.) to produce an ovicidal substance against S. furcifera eggs. Biosci. Biotechnol. Biochem. 78: 937–942.
579. Nakamura, Y. et al. Synthesis of 6-substituted 1-oxoindanoyl isoleucine conjugates and modeling studies with the COI1-JAZ co-receptor complex of lima bean. J. Chem. Ecol. in press.
580. Vandenbrink, J.P., Brown, E.A., Harmer, S.L., and Blackman, B.K. (2014). Turning heads: The biology of solar tracking in sunflower. Plant Sci. 224: 20–26.

オープンキャンパス，研究室セミナー，輪読

7/19（土）に茨城大学農学部オープンキャンパスが開催されました．研究室公開は 9:30-15:00 に行われ，午前中にはまずまずの見学者の方がお見えになりました．高校生の親子連れの見学者の方が多く，中には専門的なことにかなり突っ込んでくるお父さんもいらっしゃいました．研究棟の一階は生物制御化学研究室しかなく，やや寂しい雰囲気なので，例年見学のお客さんが少なめなのですが，今年も午後はあまりお客さんがいませんでした．

7/9 の研究室セミナーでの文献紹介の担当は四年生の渡邉さんで，紹介した論文は Rosseto, R. & Hajdu, J. Synthesis of phospholipids on a glyceric acid scaffold: design and preparation of phospholipase A2 specific substrates. Tetrahedron 70, 3155-3165 (2014) でした．ホスホリパーゼ A2 の活性測定などのプローブとして用いることができる基質アナログの合成研究についての論文です．

7/16 の研究室セミナーでの文献紹介の担当は化学生態学研究室四年生の横山さんで，紹介した論文は Paque, S. et al. AUXIN BINDING PROTEIN1 links cell wall remodeling, auxin signaling, and cell expansion in Arabidopsis. Plant Cell 26, 280-295 (2014) でした．AUXIN BINDING PROTEIN 1 の細胞伸長における機能を細胞壁のリモデリングに着目して解析した論文です．

先週及び先々週の輪読では下記の論文を取り上げました．
568. Lutz, J.A., Kulshrestha, M., Rogers, D.T., and Littleton, J.M. A nicotinic receptor-mediated anti-inflammatory effect of the flavonoid rhamnetin in BV2 microglia. Fitoterapia in press.
569. Korneli, C., Danisman, S., and Staiger, D. Differential control of pre-invasive and post-invasive antibacterial defense by the Arabidopsis circadian clock. Plant Cell Physiol. in press.
570. Al Atalah, B. et al. (2014). Transcriptional behavior of EUL-related rice lectins toward important abiotic and biotic stresses. J. Plant Physiol. 171: 986–992.
571. Lam, P.Y., Zhu, F.-Y., Chan, W.L., Liu, H., and Lo, C. (2014). Cytochrome P450 93G1 is a flavone synthase II that channels flavanones to the biosynthesis of tricin O-linked conjugates in rice. Plant Physiol. 165: 1315–1327.
572. King, A.M. et al. (2014). Aspergillomarasmine A overcomes metallo-β-lactamase antibiotic resistance. Nature 510: 503–506.
573. Appel, H.M., and Cocroft, R.B. Plants respond to leaf vibrations caused by insect herbivore chewing. Oecologia in press.
574. Monte, I. et al. (2014). Rational design of a ligand-based antagonist of jasmonate perception. Nat. Chem. Biol. 10: 671–676.
575. Zander, M., Thurow, C., and Gatz, C. TGA transcription factors activate the salicylic acid-suppressible branch of the ethylene-induced defense program by regulating ORA59 expression. Plant Physiol. in press.
576. Li, B., Bhandari, D.R., Janfelt, C., Römpp, A., and Spengler, B. Natural products in licorice (Glycyrrhiza glabra) rhizome imaged at the cellular level by atmospheric pressure Matrix-Assisted Laser Desorption/Ionization Tandem Mass Spectrometry Imaging. Plant J. in press.

研究室セミナー & 輪読

また，しばらくブログ更新をさぼってしまいました．

6/11 の研究室セミナーでの文献紹介の担当は化学生態学研究室四年生の新井君で，紹介した論文は Ko, D. et al. Arabidopsis ABCG14 is essential for the root-to-shoot translocation of cytokinin. Proc. Natl. Acad. Sci. USA 111, 7150-7155 (2014) でした．シロイヌナズナでの根からは地上部へのサイトカイニンの輸送に関与する ABC トランスポーターについての論文です．

6/18 は教員の都合でセミナーはお休みでした．

6/25 の研究室セミナーでの文献紹介の担当は四年生の酒井君で，紹介した論文は Fernandez, J. & Wilson, R. A. Characterizing roles for the glutathione reductase, thioredoxin reductase and thioredoxin peroxidase-encoding genes of Magnaporthe oryzae during rice blast disease. PLoS ONE 9, e87300 (2014) でした．イネいもち病菌の感染にグルタチオンとチオレドキシンの還元酵素が寄与していることを報告した論文です．

7/2 の研究室セミナーでの文献紹介の担当は四年生の照山君で，紹介した論文は Yoshida, M., Saito, K., Fujino, Y. & Doi, T. A concise total synthesis of biologically active frutinones via tributylphosphine-catalyzed tandem acyl transfer-cyclization. Tetrahedron 70, 3452-3458 (2014) でした．抗真菌活性を持つフラボノイド化合物フルチノン類の全合成を報告した論文です．

6/9-7/4 の輪読では下記の論文を取り上げました．
548. Spence, C. et al. (2014). Natural rice rhizospheric microbes suppress rice blast infections. BMC Plant Biol. 14: 130. 549. Kim, Y., Komoda, E., Miyashita, M., and Miyagawa, H. (2014). Continuous stimulation of the plant immune system by the peptide elicitor PIP-1 is required for phytoalexin biosynthesis in tobacco cells. J. Agric. Food Chem. 62: 5781–5788.
550. Chujo, T. et al. (2014). Overexpression of phosphomimic mutated OsWRKY53 leads to enhanced blast resistance in rice. PLoS ONE 9: e98737.
551. Liu, W., Liu, J., Triplett, L., Leach, J.E., and Wang, G.-L. Novel insights into rice innate immunity against bacterial and fungal pathogens. Annu. Rev. Phytopathol. in press.
552. Jayaraman, D., Gilroy, S., and Ané, J.-M. (2014). Staying in touch: mechanical signals in plant–microbe interactions. Curr. Opin. Plant Biol. 20: 104–109.
553. Walters, D.R. et al. (2014). Control of foliar pathogens of spring barley using a combination of resistance elicitors. Front. Plant Sci. 5: 241.
554. Xu, E., and Brosche, M. (2014). Salicylic acid signaling inhibits apoplastic reactive oxygen species signaling. BMC Plant Biol. 14: 155.
555. Zernova, O.V. et al. (2014). Regulation of plant immunity through modulation of phytoalexin synthesis. Molecules 19: 7480–7496.
556. Nakasu, E.Y.T. et al. (2014). Novel biopesticide based on a spider venom peptide shows no adverse effects on honeybees. Proc. R. Soc. B 281: 20140619.
557. Taguchi, Y., Elsharkawy, M.M., Hassan, N., and Hyakumachi, M. (2014). A novel method for controlling rice blast disease using fan-forced wind on paddy fields. Crop Prot. 63: 68–75.
558. Tooker, J.F., and Helms, A.M. Phytohormone dynamics associated with gall insects, and their potential role in the evolution of the gall-inducing habit. J. Chem. Ecol. in press.
559. Zhang, N., Lariviere, A., Tonsor, S.J., and Traw, M.B. (2014). Constitutive camalexin production and environmental stress response variation in Arabidopsis populations from the Iberian Peninsula. Plant Sci. 225: 77–85.
560. Floková, K. et al. UHPLC–MS/MS based target profiling of stress-induced phytohormones. Phytochemistry in press.
561. Misra, B.B., Assmann, S.M., and Chen, S. Plant single-cell and single-cell-type metabolomics. Trends Plant Sci. in press.
562. Yang, X., Neta, P., and Stein, S.E. (2014). Quality control for building libraries from electrospray ionization tandem mass spectra. Anal. Chem. 86: 6393–6400.
563. Palmer, A.G., Senechal, A.C., Mukherjee, A., Ané, J.-M., and Blackwell, H.E. Plant responses to bacterial N-acyl l-homoserine lactones are dependent on enzymatic degradation to l-homoserine. ACS Chem. Biol. in press.
564. Giron, D., and Glevarec, G. Cytokinin-induced phenotypes in plant-insect interactions: learning from the bacterial world. J. Chem. Ecol. in press.
565. Wei, J. et al. (2014). Reciprocal crosstalk between jasmonate and salicylate defence-signalling pathways modulates plant volatile emission and herbivore host-selection behaviour. J. Exp. Bot. 65: 3289–3298.
566. Zheng, T., and Nolan, E.M. Enterobactin-mediated delivery of β-lactam antibiotics enhances antibacterial activity against pathogenic Escherichia coli. J. Am. Chem. Soc. in press.
567. Campos, M.L., Kang, J.-H., and Howe, G.A. Jasmonate-triggered plant immunity. J. Chem. Ecol. in press.