研究室セミナー＆ 5/9-20 の輪読

5/2 で今年度の年間計画発表が終了したので，5/12 の研究室セミナーからは文献紹介が始まりました．5/12 は化学生態学研究室博士 1 年の武井さんの担当で，紹介した論文は Christensen, S.A. et al. (2015). Maize death acids, 9-lipoxygenase–derived cyclopente(a)nones, display activity as cytotoxic phytoalexins and transcriptional mediators. Proc. Natl. Acad. Sci. USA 112: 11407–11412でした．5/19 は修士 2 年の武山君の担当で，紹介した論文は Chanclud, E. et al. (2016). Cytokinin production by the rice blast fungus is a pivotal requirement for full virulence. PLoS Pathoge. 12: e1005457 でした．

5/9-20 の輪読では下記の論文を取り上げました．

938. Zimmermann, M.R., Mithöfer, A., Will, T., Felle, H.H., and Furch, A.C.U. (2016). Herbivore-triggered electrophysiological reactions: candidates for systemic signals in higher plants and the challenge of their identification. Plant Physiol. 170: 2407–2419.

939. Li, L., Chen, X., Ma, C., Wu, H., and Qi, S. (2016). Piriformospora indica requires kaurene synthase activity for successful plant colonization. Plant Physiol. Biochem. 102: 151–160.

940. Sugano, S. et al. (2016). Rice OsVAMP714, a membrane-trafficking protein localized to the chloroplast and vacuolar membrane, is involved in resistance to rice blast disease. Plant Mol. Biol. 91: 81–95.

941. Tsou, L.K. et al. (2016). Antibacterial flavonoids from medicinal plants covalently inactivate type III protein secretion substrates. J. Am. Chem. Soc. 138: 2209–2218.

942. Chanclud, E. et al. (2016). Cytokinin production by the rice blast fungus is a pivotal requirement for full virulence. PLoS Pathog. 12: e1005457.

943. Yi, J. et al. (2016). OsMPK6 plays a critical role in cell differentiation during early embryogenesis in Oryza sativa. J. Exp. Bot. 67: 2425–2437.

944. Takagi, H. et al. (2016). Allantoin, a stress-related purine metabolite, can activate jasmonate signaling in a MYC2-regulated and abscisic acid-dependent manner. J. Exp. Bot. 67: 2519–2532.

945. Tian, S. et al. Plant aquaporin AtPIP1;4 links apoplastic H2O2 induction to disease immunity pathways. Plant Physiol. in press.

946. Kumari, C., Dutta, T.K., Banakar, P., and Rao, U. (2016). Comparing the defence-related gene expression changes upon root-knot nematode attack in susceptible versus resistant cultivars of rice. Sci. Rep. 6: 22846.

4/25-5/6 の輪読

4/25-5/6 の輪読では下記の論文を取り上げました．

932. Flamini, R. et al. (2016). Stilbene oligomer phytoalexins in grape as a response to A. carbonarius infection. Physiol. Mol. Plant Pathol. 93: 112–118.

933. Wang, S. et al. (2016). α-Ketol linolenic acid (KODA) application affects endogenous abscisic acid, jasmonic acid and aromatic volatiles in grapes infected by a pathogen (Glomerella cingulata). J. Plant Physiol. 192: 90–97.

934. Dastmalchi, M., Bernards, M., and Dhaubhadel, S. (2016). Twin anchors of the soybean isoflavonoid metabolon: evidence for tethering of the complex to the endoplasmic reticulum by IFS and C4H. Plant J. 85: 689–706.

935. Kim, S.H., Qi, D., Ashfield, T., Helm, M., and Innes, R.W. (2016). Using decoys to expand the recognition specificity of a plant disease resistance protein. Science 351: 684–687.

936. Pelagio-Flores, R., Ruiz-Herrera, L.F., and López-Bucio, J. Serotonin modulates Arabidopsis root growth via changes in reactive oxygen species and jasmonic acid–ethylene signaling. Physiol. Plant. in press.

937. Tamiru, M. et al. A chloroplast-localized protein LESION AND LAMINA BENDING affects defence and growth responses in rice. New Phytol. in press.

堀江清孝君の論文が Phytochemistry Letters 誌に掲載．

ブログでの報告が遅れていましたが，この 3 月に博士の学位を取得して連合大学院を修了した堀江清孝君が筆頭著者の論文がヨーロッパ植物化学会の英文誌 Phytochemistry Letters の 2016 年 3 月号に掲載されました．

Horie, K., Sakai, K., Okugi, M., Toshima, H., and Hasegawa, M. (2016). Ultraviolet-induced amides and casbene diterpenoids from rice leaves. Phytochem. Lett. 15: 57–62.

この研究成果は堀江君の博士論文研究の一部ですが，卒業生の酒井健伍君と奥木美咲さんの卒業論文の成果も含まれています．

紫外線照射したイネ葉片に含まれる二次代謝産物を LC/MS を用いてプロファイリングし，従来はイネからは知られていなかった化合物 3 つを発見しました．一つはたの植物からはすでに単離が報告されている N-ベンゾイルチラミンでした．あとの 2 つはカスベン骨格を持つ新規ジテルペン化合物で．2013 年に本研究室の井上靖乃さんが報告したフィトアレキシンである ent-10-オキソデプレッシン の生合成前駆体と考えられるものでした．また，今回発見した化合物を含めて既知のイネフィトアレキシンの蓄積量といもち病菌に対する抗菌活性を再評価し，2 種類のアミド化合物（N-ベンゾイルトリプタミン，N-シナモイルトリプタミン）がいもち病菌に対するフィトアレキシンとして機能していることも明らかにしました．

今週の輪読

今週の輪読では下記の論文を取り上げました．

927. Hummel, A.W., Wilkins, K.E., Wang, L., Cernadas, R.A., and Bogdanove, A.J. A transcription activator-like effector from Xanthomonas oryzae pv. oryzicola elicits dose-dependent resistance in rice. Mol. Plant Pathol. in press.

928. Wu, L., Wu, H., Chen, L., Zhang, H., and Gao, X. Induction of systemic disease resistance in Nicotiana benthamiana by the cyclodipeptides cyclo (L-Pro-L-Pro) and cyclo (D-Pro-D-Pro). Mol. Plant Pathol. in press.

929. Babino, D. et al. (2016). The biochemical basis of vitamin A3 production in arthropod vision. ACS Chem. Biol. 11: 1049–1057.

930. Kettle, A.J. et al. (2016). The Fdb3 transcription factor of the Fusarium Detoxification of Benzoxazolinone gene cluster is required for MBOA but not BOA degradation in Fusarium pseudograminearum. Fungal Genet. Biol. 88: 44–53.

931. Barrios-Collado, C. et al. (2016). Capturing in vivo plant metabolism by real-time analysis of low to high molecular weight volatiles. Anal. Chem. 88: 2406–2412.

今週の輪読

今週の輪読では下記の論文を取り上げました．

922. Pérez-Bueno, M.L. et al. (2016). Temporal and spatial resolution of activated plant defense responses in leaves of Nicotiana benthamiana infected with Dickeya dadantii. Front. Plant Sci. 6: 1209.

923. Harkenrider, M. et al. (2016). Overexpression of rice wall-associated kinase 25 (OsWAK25) alters resistance to bacterial and fungal pathogens. PLoS ONE 11: e0147310.

924. Abeysekara, N.S., Swaminathan, S., Desai, N., Guo, L., and Bhattacharyya, M.K. (2016). The plant immunity inducer pipecolic acid accumulates in the xylem sap and leaves of soybean seedlings following Fusarium virguliforme infection. Plant Sci. 243: 105–114.

925. Kumar, Y. et al. Fusarium oxysporum mediates systems metabolic reprogramming of chickpea roots as revealed by a combination of proteomics and metabolomics. Plant Biotechnol. J. in press.

926. Chapman, A., Lindermayr, C., and Glawischnig, E. (2016). Expression of antimicrobial peptides under control of a camalexin-biosynthetic promoter confers enhanced resistance against Pseudomonas syringae. Phytochemistry 122: 76–80.

先週の輪読

4/4 から今年度の輪読を始めました．先週の輪読では下記の論文を取り上げました（先週の輪読はお休み）．

917. Rodriguez-Salus, M. et al. (2016). The synthetic elicitor 2-(5-bromo-2-hydroxy-phenyl)-thiazolidine-4-carboxylic acid links plant immunity to hormesis. Plant Physiol. 170: 444–458.

918. Dobritzsch, M. et al. (2016). MATE transporter-dependent export of hydroxycinnamic acid amides. Plant Cell 28: 583–596.

919. Heiling, S. et al. (2016). Using the knowns to discover the unknowns: MS-based dereplication uncovers structural diversity in 17-hydroxygeranyllinalool diterpene glycoside defense production in the Solanaceae. Plant J. 85: 561–577.

920. Scholz, S.S., Reichelt, M., Mekonnen, D.W., Ludewig, F., and Mithöfer, A. (2015). Insect herbivory-elicited GABA accumulation in plants is a wound-induced, direct, systemic and jasmonate-independent defense response. Front. Plant Sci. 6: 1128.

921. Wei, T., Chern, M., Liu, F., and Ronald, P.C. Suppression of bacterial infection in rice by treatment with a sulfated peptide. Mol. Plant Pathol. in press.

新年度，日本農芸化学会，輪読

新年度が始まりました．今年度の生物制御化学研究室は修士課程の学生が 2 年生 2 名，1 年生 2 名，学部 4 年生が 4 名という体制です．

3 月 27 日～ 30 日には札幌コンベンションセンターなどで日本農芸化学会 2016 年度大会が開催され，生物制御化学研究室からは 2 題の発表を行いました．

• 3 月 29 日（火）
  
  • 3B020 イネフィトアレキシン モミラクトン A のイネいもち病菌による代謝産物の同定
    
  • ○武山恵典，平石由貴，遠藤尚，今井卓也，戸嶋浩明，長谷川守文（茨城大農）
    
• 3 月 30 日（水）
  
  • 4B068 シクロペンタン導入型 KODA 類縁体の合成研究
    
  • ○渡辺美聡，横山峰幸，長谷川守文，戸嶋浩明（茨城大農，横浜市大）
    

3/22〜3/25 の輪読では下記の論文を取り上げました（先週の輪読はお休み）．

913. Zeier, J. et al. (2016). Pipecolic acid orchestrates plant systemic acquired resistance and defense priming via salicylic acid-dependent and independent pathways. Plant Cell 28: 102–129.

914. Iwabuchi, K., Hidema, J., Tamura, K., Takagi, S., and Hara-Nishimura, I. (2016). Plant nuclei move to escape ultraviolet-induced DNA damage and cell death. Plant Physiol. 170: 678–685.

915. Yoshitomi, K. et al. (2016). Rice terpene synthase 24 (OsTPS24) encodes a jasmonate-responsive monoterpene synthase that produces an antibacterial γ-terpinene against rice pathogen. J. Plant Physiol. 191: 120–126.

916. Zebelo, S., Song, Y., Kloepper, J.W., and Fadamiro, H. (2016). Rhizobacteria activates (+)-δ-cadinene synthase genes and induces systemic resistance in cotton against beet armyworm (Spodoptera exigua). Plant Cell Environ. 39: 935–943.