昨日の 12/27 で大学は仕事納めでした．来年の仕事始めは 1/6 です．
今週の輪読では下記の論文を取り上げました．
458. Sohn, S.-I. et al. (2014). Genistein production in rice seed via transformation with soybean IFS genes. Plant Sci. 217-218: 27–35.
459. Pedras, M.S.C., Minic, Z., and Abdoli, A. The phytoalexin camalexin induces fundamental changes in the proteome of Alternaria brassicicola different from those caused by brassinin. Fungal Biology in press.
460. Celoy, R.M., and VanEtten, H.D. (+)-Pisatin biosynthesis: From (–) enantiomeric intermediates via an achiral 7,2´-dihydroxy-4´,5´-methylenedioxyisoflav-3-ene. Phytochemistry in press.
461. Velema, W.A. et al. (2013). Optical control of antibacterial activity. Nat. Chem. 5: 924–928.
2013年12月29日日曜日
2013年12月21日土曜日
研究室セミナー & 今週の輪読
今週の研究室セミナーでの文献紹介の担当は化学生態学研究室四年生の武井さんで，紹介した論文は Abe, H. et al. Disarming the jasmonate-dependent plant defense makes nonhost Arabidopsis plants accessible to the American serpentine leafminer. Plant Physiol. 163, 1242-1253 (2013) でした．シロイヌナズナのジャスモン酸非感受性変異体 coi1-1 が野生型は食害を受けないマメハモグリバエの食害を受けることになることについての報告です．今週で今年の研究室セミナーは終了です．今年度の文献紹介も終了で，年明けからは研究報告のみのセミナーを行います．
今週の輪読では下記の論文を取り上げました．
453. Nakano, H. et al. (2013). Isolation and identification of phenolic compounds accumulated in brown rice grains ripened under high air temperature. J. Agric. Food Chem. 61: 11921–11928.
454. Groen, S.C. et al. Pathogen-triggered ethylene signaling mediates systemic-induced susceptibility to herbivory in Arabidopsis. Plant Cell in press.
455. Xiao, J. et al. (2013). Rice WRKY13 regulates cross talk between abiotic and biotic stress signaling pathways by selective binding to different cis-elements. Plant Physiol. 163: 1868–1882.
456. Nishimura, T. et al. YUCASIN is a potent inhibitor of YUCCA(s), a key enzyme in auxin biosynthesis. Plant J. in press.
457. Wankhede, D., Kumar, K., Singh, P., and Sinha, A. (2013). Involvement of Mitogen activated protein Kinase Kinase 6 in UV induced transcripts accumulation of genes in phytoalexin biosynthesis in rice. Rice 6: 35.
今週の輪読では下記の論文を取り上げました．
453. Nakano, H. et al. (2013). Isolation and identification of phenolic compounds accumulated in brown rice grains ripened under high air temperature. J. Agric. Food Chem. 61: 11921–11928.
454. Groen, S.C. et al. Pathogen-triggered ethylene signaling mediates systemic-induced susceptibility to herbivory in Arabidopsis. Plant Cell in press.
455. Xiao, J. et al. (2013). Rice WRKY13 regulates cross talk between abiotic and biotic stress signaling pathways by selective binding to different cis-elements. Plant Physiol. 163: 1868–1882.
456. Nishimura, T. et al. YUCASIN is a potent inhibitor of YUCCA(s), a key enzyme in auxin biosynthesis. Plant J. in press.
457. Wankhede, D., Kumar, K., Singh, P., and Sinha, A. (2013). Involvement of Mitogen activated protein Kinase Kinase 6 in UV induced transcripts accumulation of genes in phytoalexin biosynthesis in rice. Rice 6: 35.
2013年12月15日日曜日
研究室セミナー & 輪読
先週の研究室セミナーでの文献紹介の担当は四年生の奥木さんで，紹介した論文は Stallforth, P. et al. A bacterial symbiont is converted from an inedible producer of beneficial molecules into food by a single mutation in the gacA gene. Proc. Natl. Acad. Sci. USA 110, 14528-14533 (2013) でした．キイロタマホロリカビの共生細菌が，単一の変異によって，キイロタマホコリカビの生存に有利な二次代謝産物を生産する共生関係からキイロタマホコリカビの食料になるという報告です．今週の担当は四年生の遠藤君で，紹介した論文は Mousavi, S. A. R., Chauvin, A., Pascaud, F., Kellenberger, S. & Farmer, E. E. GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling. Nature 500, 422-426 (2013) でした．傷害ストレスシグナルの葉から葉への伝達にはグルタミン酸受容体様タンパク質を介した電気的シグナルが関与していることを報告した論文です．
先週，今週の輪読では下記の論文を取り上げました．
443. Shimada, T.L. et al. Leaf oil body functions as a subcellular factory for the production of a phytoalexin in Arabidopsis thaliana. Plant Physiol. in press.
444. Pedras, M.S.C., and Minic, Z. The phytoalexins brassilexin and camalexin inhibit cyclobrassinin hydrolase, a unique enzyme from the fungal pathogen Alternaria brassicicola. Bioorg. Med. Chem. in press.
445. Zhang, Q.-F., Fu, Y.-J., Huang, Z.-W., Shangguang, X.-C., and Guo, Y.-X. (2013). Aqueous stability of astilbin: effects of pH, temperature, and solvent. J. Agric. Food Chem. 61: 12085–12091.
446. Nishizaki, Y. et al. (2013). p-Hydroxybenzoyl-glucose is a zwitter donor for the biosynthesis of 7-polyacylated anthocyanin in delphinium. Plant Cell 25: 4150–4165.
447. Pĕnčík, A. et al. (2013). Regulation of auxin homeostasis and gradients in arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell 25: 3858–3870.
448. Onoyovwe, A. et al. (2013). Morphine biosynthesis in opium poppy involves two cell types: sieve elements and laticifers. Plant Cell 25: 4110–4122.
449. Keereetaweep, J., Blancaflor, E.B., Hornung, E., Feussner, I., and Chapman, K.D. (2013). Ethanolamide oxylipins of linolenic acid can negatively regulate Arabidopsis seedling development. Plant Cell 25: 3824–3840.
450. Martellini, F. et al. Can adaptation to metalliferous environments affect plant response to biotic stress? Insight from Silene paradoxa L. and phytoalexins. Environ. Exp. Bot. in press.
451. Qian, Q., Huang, L., Yi, R., Wang, S., and Ding, Y. Enhanced resistance to blast fungus in rice (Oryza sativa L.) by expressing the ribosome-inactivating protein alpha-momorcharin. Plant Sci. in press.
452. Li, T. et al. Jumonji C protein JMJ705-mediated removal of histone H3 lysine 27 trimethylation is involved in defense-related gene activation in rice. Plant Cell in press.
先週，今週の輪読では下記の論文を取り上げました．
443. Shimada, T.L. et al. Leaf oil body functions as a subcellular factory for the production of a phytoalexin in Arabidopsis thaliana. Plant Physiol. in press.
444. Pedras, M.S.C., and Minic, Z. The phytoalexins brassilexin and camalexin inhibit cyclobrassinin hydrolase, a unique enzyme from the fungal pathogen Alternaria brassicicola. Bioorg. Med. Chem. in press.
445. Zhang, Q.-F., Fu, Y.-J., Huang, Z.-W., Shangguang, X.-C., and Guo, Y.-X. (2013). Aqueous stability of astilbin: effects of pH, temperature, and solvent. J. Agric. Food Chem. 61: 12085–12091.
446. Nishizaki, Y. et al. (2013). p-Hydroxybenzoyl-glucose is a zwitter donor for the biosynthesis of 7-polyacylated anthocyanin in delphinium. Plant Cell 25: 4150–4165.
447. Pĕnčík, A. et al. (2013). Regulation of auxin homeostasis and gradients in arabidopsis roots through the formation of the indole-3-acetic acid catabolite 2-oxindole-3-acetic acid. Plant Cell 25: 3858–3870.
448. Onoyovwe, A. et al. (2013). Morphine biosynthesis in opium poppy involves two cell types: sieve elements and laticifers. Plant Cell 25: 4110–4122.
449. Keereetaweep, J., Blancaflor, E.B., Hornung, E., Feussner, I., and Chapman, K.D. (2013). Ethanolamide oxylipins of linolenic acid can negatively regulate Arabidopsis seedling development. Plant Cell 25: 3824–3840.
450. Martellini, F. et al. Can adaptation to metalliferous environments affect plant response to biotic stress? Insight from Silene paradoxa L. and phytoalexins. Environ. Exp. Bot. in press.
451. Qian, Q., Huang, L., Yi, R., Wang, S., and Ding, Y. Enhanced resistance to blast fungus in rice (Oryza sativa L.) by expressing the ribosome-inactivating protein alpha-momorcharin. Plant Sci. in press.
452. Li, T. et al. Jumonji C protein JMJ705-mediated removal of histone H3 lysine 27 trimethylation is involved in defense-related gene activation in rice. Plant Cell in press.
2013年12月2日月曜日
化学生態学実験 III，研究室セミナー，輪読
しばらくブログ更新をさぼってしまいました．長谷川担当の化学生態学実験 III は 11/22 で全日程を終了しました．GC/MS で籾殻抽出物中のモミラクトン A を検出する実験が一回目はどの班でも検出できないという問題がありましたが，濃度を上げたところ，全ての班が検出することができました．それ以外は特に大きなトラブルもなく，順調に終了しました．TA の大学院生の堀江君と濱名君のおかげです．レポート提出〆切は今週末 12/6 です．
先々週の研究室セミナーでの文献紹介の担当は四年生の渡辺さんで，紹介した論文は Zhang, C., Ma, X. & Du, Y. Synthesis of Neu5Ac-α-C-galactopyranosyl-functionalized magnetic nanoparticles via click chemistry. Tetrahedron 69, 7300-7307 (2013) でした．クリックケミストリーによって N-アセチルノイラミン酸を結合させた磁性ナノ粒子の調製についての論文です．先週の担当は化学生態学研究室四年生の藤原さんで，紹介した論文は Sugawara, K. et al. The alteration of plant morphology by small peptides released from the proteolytic processing of the bacterial peptide TENGU. Plant Physiol. 162, 2005-2014 (2013) でした．天狗巣病の原因ペプチド TENGU の植物体内でのタンパク質分解酵素によるプロセシングについての論文です．
先々週，先週の輪読では下記の論文を取り上げました．
433. Chooi, Y.-H., Hong, Y.J., Cacho, R.A., Tantillo, D.J., and Tang, Y. (2013). A cytochrome P450 serves as an unexpected terpene cyclase during fungal meroterpenoid biosynthesis. J. Am. Chem. Soc. 135: 16805–16808.
434. Cai, F. et al. (2013). Harzianolide, a novel plant growth regulator and systemic resistance elicitor from Trichoderma harzianum. Plant Physiol. Biochem. 73: 106–113.
435. Pérez-Trujillo, M., Monteagudo, E., and Parella, T. (2013). 13C NMR spectroscopy for the differentiation of enantiomers using chiral solvating agents. Anal. Chem. 85: 10887–10894.
436. Bandurska, H., and Cieślak, M. (2013). The interactive effect of water deficit and UV-B radiation on salicylic acid accumulation in barley roots and leaves. Environ. Exp. Bot. 94: 9–18.
437. Rodríguez, A. et al. Terpene downregulation triggers defense responses in transgenic oranges leading to resistance against fungal pathogens. Plant Physiol. in press.
438. Nuruzzaman, M. et al. Comparative transcriptome profiles of the WRKY gene family under control, hormone-treated, and drought conditions in near-isogenic rice lines reveal differential, tissue specific gene activation. J. Plant Physiol. in press.
439. Kurepa, J. et al. Direct isolation of flavonoids from plants using ultra-small anatase TiO2 nanoparticles. Plant J. in press.
440. Goufo, P. et al. (2014). Rice (Oryza sativa L.) phenolic compounds under elevated carbon dioxide (CO2) concentration. Environ. Exp. Bot. 99: 28–37.
441. Kolev, J.N., O’Dwyer, K.M., Jordan, C.T., and Fasan, R. Discovery of potent parthenolide-based antileukemic agents enabled by late-stage P450-mediated C–H functionalization. ACS Chem. Biol. in press.
442. Pollier, J. et al. The protein quality control system manages plant defence compound synthesis. Nature in press.
先々週の研究室セミナーでの文献紹介の担当は四年生の渡辺さんで，紹介した論文は Zhang, C., Ma, X. & Du, Y. Synthesis of Neu5Ac-α-C-galactopyranosyl-functionalized magnetic nanoparticles via click chemistry. Tetrahedron 69, 7300-7307 (2013) でした．クリックケミストリーによって N-アセチルノイラミン酸を結合させた磁性ナノ粒子の調製についての論文です．先週の担当は化学生態学研究室四年生の藤原さんで，紹介した論文は Sugawara, K. et al. The alteration of plant morphology by small peptides released from the proteolytic processing of the bacterial peptide TENGU. Plant Physiol. 162, 2005-2014 (2013) でした．天狗巣病の原因ペプチド TENGU の植物体内でのタンパク質分解酵素によるプロセシングについての論文です．
先々週，先週の輪読では下記の論文を取り上げました．
433. Chooi, Y.-H., Hong, Y.J., Cacho, R.A., Tantillo, D.J., and Tang, Y. (2013). A cytochrome P450 serves as an unexpected terpene cyclase during fungal meroterpenoid biosynthesis. J. Am. Chem. Soc. 135: 16805–16808.
434. Cai, F. et al. (2013). Harzianolide, a novel plant growth regulator and systemic resistance elicitor from Trichoderma harzianum. Plant Physiol. Biochem. 73: 106–113.
435. Pérez-Trujillo, M., Monteagudo, E., and Parella, T. (2013). 13C NMR spectroscopy for the differentiation of enantiomers using chiral solvating agents. Anal. Chem. 85: 10887–10894.
436. Bandurska, H., and Cieślak, M. (2013). The interactive effect of water deficit and UV-B radiation on salicylic acid accumulation in barley roots and leaves. Environ. Exp. Bot. 94: 9–18.
437. Rodríguez, A. et al. Terpene downregulation triggers defense responses in transgenic oranges leading to resistance against fungal pathogens. Plant Physiol. in press.
438. Nuruzzaman, M. et al. Comparative transcriptome profiles of the WRKY gene family under control, hormone-treated, and drought conditions in near-isogenic rice lines reveal differential, tissue specific gene activation. J. Plant Physiol. in press.
439. Kurepa, J. et al. Direct isolation of flavonoids from plants using ultra-small anatase TiO2 nanoparticles. Plant J. in press.
440. Goufo, P. et al. (2014). Rice (Oryza sativa L.) phenolic compounds under elevated carbon dioxide (CO2) concentration. Environ. Exp. Bot. 99: 28–37.
441. Kolev, J.N., O’Dwyer, K.M., Jordan, C.T., and Fasan, R. Discovery of potent parthenolide-based antileukemic agents enabled by late-stage P450-mediated C–H functionalization. ACS Chem. Biol. in press.
442. Pollier, J. et al. The protein quality control system manages plant defence compound synthesis. Nature in press.
2013年11月16日土曜日
化学生態学実験 III，研究室セミナー，今週の輪読
今週の月曜日から長谷川が担当する資源生物科学科三年生向けの学生実験「化学生態学実験 III」が始まりました．今年の受講者は 27 名です．今日で前半を終了しましたが，ここまではまだ結果が何も出ていない状態なので，うまくいっているかどうかははっきりしませんが，見たところまあまあ順調に進んでいるようです．
水曜日の研究室セミナーでの文献紹介の担当は化学生態学研究室四年生の鈴木君で，紹介した論文は Sairanen, I. et al. Soluble carbohydrates regulate auxin biosynthesis via PIF proteins in Arabidopsis. Plant Cell 24, 4907-4916 (2012) でした．糖によってオーキシンの生合成が制御され，その制御には PIF タンパク質が関わっていることを報告した論文です．
今週の輪読では下記の論文を取り上げました．
428. Ma, Y., Zhao, Y., Walker, R.K., and Berkowitz, G.A. (2013). Molecular steps in the immune signaling pathway evoked by plant elicitor peptides: Ca2+-dependent protein kinases, nitric oxide, and reactive oxygen species are downstream from the early Ca2+ signal. Plant Physiol. 163: 1459–1471.
429. Abe, H. et al. (2013). Disarming the jasmonate-dependent plant defense makes nonhost Arabidopsis plants accessible to the American serpentine leafminer. Plant Physiol. 163: 1242–1253.
430. Gupta, O.P., Sharma, P., Gupta, R.K., and Sharma, I. (2014). Current status on role of miRNAs during plant-fungus interaction. Physiol. Mol. Plant Pathol. 85: 1–7.
431. Hatmi, S. et al. Osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to Botrytis cinerea. J. Exp. Bot. in press.
432. Friedman, M. (2013). Rice brans, rice bran oils, and rice hulls: composition, food and industrial uses, and bioactivities in humans, animals, and cells. J. Agric. Food Chem. 61: 10626–10641.
水曜日の研究室セミナーでの文献紹介の担当は化学生態学研究室四年生の鈴木君で，紹介した論文は Sairanen, I. et al. Soluble carbohydrates regulate auxin biosynthesis via PIF proteins in Arabidopsis. Plant Cell 24, 4907-4916 (2012) でした．糖によってオーキシンの生合成が制御され，その制御には PIF タンパク質が関わっていることを報告した論文です．
今週の輪読では下記の論文を取り上げました．
428. Ma, Y., Zhao, Y., Walker, R.K., and Berkowitz, G.A. (2013). Molecular steps in the immune signaling pathway evoked by plant elicitor peptides: Ca2+-dependent protein kinases, nitric oxide, and reactive oxygen species are downstream from the early Ca2+ signal. Plant Physiol. 163: 1459–1471.
429. Abe, H. et al. (2013). Disarming the jasmonate-dependent plant defense makes nonhost Arabidopsis plants accessible to the American serpentine leafminer. Plant Physiol. 163: 1242–1253.
430. Gupta, O.P., Sharma, P., Gupta, R.K., and Sharma, I. (2014). Current status on role of miRNAs during plant-fungus interaction. Physiol. Mol. Plant Pathol. 85: 1–7.
431. Hatmi, S. et al. Osmotic stress-induced polyamine oxidation mediates defence responses and reduces stress-enhanced grapevine susceptibility to Botrytis cinerea. J. Exp. Bot. in press.
432. Friedman, M. (2013). Rice brans, rice bran oils, and rice hulls: composition, food and industrial uses, and bioactivities in humans, animals, and cells. J. Agric. Food Chem. 61: 10626–10641.
2013年11月9日土曜日
送風乾燥機修理 & 今週の輪読
研究室では長谷川が学生の頃から 20 年以上使っているアドバンテックの送風乾燥機があるのですが，しばらく前からファンがイオンを出すようになっていて，9 月末には時折ファンが停止するような状態になってしまいました．古い機器なので，もう修理は無理かもしれないと半分諦めながらメーカーに問い合わせてみましたところ，ファンモーターの故障ならまだ修理可能ということで，引き取り修理をお願いしていました．その乾燥機が木曜日に一ヶ月ぶりに戻ってきました．戻ってきた乾燥機はファンの音が非常に静かになっていて，周りがうるさい実験室では動いているのが分からないぐらいになりました．ここのところ，製氷機，冷却水循環装置，送風乾燥機と立て続けに研究室のベテラン機器（いずれも 20 年以上使用）が不調に陥っていますが，それぞれ何とか使い続けることができています．
今週の輪読では下記の論文を取り上げました．
424. Thi, D.T., Shimatani, Z., Kawano, Y., Terada, R., and Shimamoto, K. Gene editing a constitutively active OsRac1 by homologous recombination-based gene targeting induces immune responses in rice. Plant Cell Physiol. in press.
425. Rühmann, S. et al. (2013). Induction of stilbene phytoalexins in grapevine (Vitis vinifera) and transgenic stilbene synthase-apple plants (Malus domestica) by a culture filtrate of Aureobasidium pullulans. Plant Physiol. Biochem. 72: 62–71.
426. Agati, G. et al. (2013). Functional roles of flavonoids in photoprotection: New evidence, lessons from the past. Plant Physiol. Biochem. 72: 35–45.
427. Zhang, X.- et al. The effect of the skeleton structure of flavanone and flavonoid on interaction with transferrin. Bioorg. Med. Chem. Lett. in press.
今週の輪読では下記の論文を取り上げました．
424. Thi, D.T., Shimatani, Z., Kawano, Y., Terada, R., and Shimamoto, K. Gene editing a constitutively active OsRac1 by homologous recombination-based gene targeting induces immune responses in rice. Plant Cell Physiol. in press.
425. Rühmann, S. et al. (2013). Induction of stilbene phytoalexins in grapevine (Vitis vinifera) and transgenic stilbene synthase-apple plants (Malus domestica) by a culture filtrate of Aureobasidium pullulans. Plant Physiol. Biochem. 72: 62–71.
426. Agati, G. et al. (2013). Functional roles of flavonoids in photoprotection: New evidence, lessons from the past. Plant Physiol. Biochem. 72: 35–45.
427. Zhang, X.- et al. The effect of the skeleton structure of flavanone and flavonoid on interaction with transferrin. Bioorg. Med. Chem. Lett. in press.
2013年11月7日木曜日
研究室セミナー
本日の研究室セミナーでの文献紹介の担当は四年生の五十嵐さんで，紹介した論文は Nishimura, T. et al. Xanthouroleuconaphin: a yellowish pigment from the aphid Uroleucon nigrotuberculatum and its total synthesis. Tetrahedron 69, 1808-1814 (2013) でした．セイタカアワダチソウヒゲナガアブラムシの黄色色素の単離とその全合成についての論文です．
2013年11月2日土曜日
研究室セミナー & 今週の輪読
水曜日の研究室セミナーでの文献紹介の担当は修士一年の濱名君で，紹介した論文は Lang, M. et al. Mutations in the neverland gene turned Drosophila pachea into an obligate specialist species. Science 337, 1658-1661 (2012) でした．パキアショウジョウバエが特定のサボテンしか餌にできなくなった原因は脱皮ホルモンであるエクジソン生合成に関わる酵素遺伝子の変異であったという内容の論文です．
今週の輪読では下記の論文を取り上げました．
419. Takeuchi, H., Kato, H., and Kurahashi, T. (2013). 2,4,6-Trichloroanisole is a potent suppressor of olfactory signal transduction. Proc. Natl. Acad. Sci. USA 110: 16235–16240.
420. Atwell, B.J., Han, W., and Scafaro, A.P. Could abiotic stress tolerance in wild relatives of rice be used to improve Oryza sativa? Plant Sci. in press.
421. Kain, P. et al. (2013). Odour receptors and neurons for DEET and new insect repellents. Nature 502: 507–512.
422. Barbieri, R.F., Lester, P.J., Miller, A.S., and Ryan, K.G. (2013). A neurotoxic pesticide changes the outcome of aggressive interactions between native and invasive ants. Proc. R. Soc. B 280: 20132157.
423. Höll, J. et al. The R2R3-MYB transcription factors MYB14 and MYB15 regulate stilbene biosynthesis in Vitis vinifera. Plant Cell in press.
今週の輪読では下記の論文を取り上げました．
419. Takeuchi, H., Kato, H., and Kurahashi, T. (2013). 2,4,6-Trichloroanisole is a potent suppressor of olfactory signal transduction. Proc. Natl. Acad. Sci. USA 110: 16235–16240.
420. Atwell, B.J., Han, W., and Scafaro, A.P. Could abiotic stress tolerance in wild relatives of rice be used to improve Oryza sativa? Plant Sci. in press.
421. Kain, P. et al. (2013). Odour receptors and neurons for DEET and new insect repellents. Nature 502: 507–512.
422. Barbieri, R.F., Lester, P.J., Miller, A.S., and Ryan, K.G. (2013). A neurotoxic pesticide changes the outcome of aggressive interactions between native and invasive ants. Proc. R. Soc. B 280: 20132157.
423. Höll, J. et al. The R2R3-MYB transcription factors MYB14 and MYB15 regulate stilbene biosynthesis in Vitis vinifera. Plant Cell in press.
2013年10月26日土曜日
冷却水循環装置修理 & 今週の輪読
研究室ではエバポレーターの冷却水を循環させるために東京理化の冷却水循環装置が常時 3 台動いています．しかし，その中の一台はもう 20 年以上使っている者で，かなり老朽化していました．この古い冷却水循環装置が 10 月初めから電源が入らなくなって使えなくなってしまいました．さすがにもう修理はできないかと思ったのですが，念のために東京理化に問い合わせたところ，ブレーカーを交換すれば直るかもしれないということになり，今日エンジニアの方に修理してもらいました．予想通りブレーカーの交換で直ったのですが，循環ポンプがかなり危険な状態（漏電スレスレ）なので，もう使わない方が良いと言われてしまいました．さすがに，この循環ポンプはもう修理できないとのことでした．しかし，冷却機の方は生きているので，普段は使っていない開放系用冷却水循環装置（冷却能力が弱い）を循環ポンプとして使うことにして，2 台を繋いで当面は使い続けることにしました．場所をとるのが難点ですが，とりあえず順調に動いています．予算に余裕があれば新品購入したいところですが，今年度はまだ半年近くあるので，現時点での新品購入は難しいのです．エンジニアに来てもらったついでに，まだ 2, 3 年しか使っていない新しい冷却水循環装置も不調だったので，これも見てもらって快調になりました．
今週の輪読では下記の論文を取り上げました．
414. Huda, K.M.K. et al. OsACA6, a P-type IIB Ca2+ATPase promotes salinity and drought stress tolerance in tobacco by ROS scavenging and enhancing stress-responsive genes. Plant J. in press.
415. Samalova, M., Meyer, A.J., Gurr, S.J., and Fricker, M.D. (2013). Robust anti-oxidant defences in the rice blast fungus Magnaporthe oryzae confer tolerance to the host oxidative burst. New Phytol. in press.
416. Du, H., Liu, H., and Xiong, L. (2013). Endogenous auxin and jasmonic acid levels are differentially modulated by abiotic stresses in rice. Front. Plant Sci. 4: 397.
417. Oka, K., Kobayashi, M., Mitsuhara, I., and Seo, S. Jasmonic acid negatively regulates resistance to Tobacco mosaic virus in tobacco. Plant Cell Physiol. in press.
418. Bryden, J., Gill, R.J., Mitton, R.A.A., Raine, N.E., and Jansen, V.A.A. Chronic sublethal stress causes bee colony failure. Ecol. Lett. in press.
今週の輪読では下記の論文を取り上げました．
414. Huda, K.M.K. et al. OsACA6, a P-type IIB Ca2+ATPase promotes salinity and drought stress tolerance in tobacco by ROS scavenging and enhancing stress-responsive genes. Plant J. in press.
415. Samalova, M., Meyer, A.J., Gurr, S.J., and Fricker, M.D. (2013). Robust anti-oxidant defences in the rice blast fungus Magnaporthe oryzae confer tolerance to the host oxidative burst. New Phytol. in press.
416. Du, H., Liu, H., and Xiong, L. (2013). Endogenous auxin and jasmonic acid levels are differentially modulated by abiotic stresses in rice. Front. Plant Sci. 4: 397.
417. Oka, K., Kobayashi, M., Mitsuhara, I., and Seo, S. Jasmonic acid negatively regulates resistance to Tobacco mosaic virus in tobacco. Plant Cell Physiol. in press.
418. Bryden, J., Gill, R.J., Mitton, R.A.A., Raine, N.E., and Jansen, V.A.A. Chronic sublethal stress causes bee colony failure. Ecol. Lett. in press.
2013年10月24日木曜日
研究室セミナー
先週は台風接近で中止になったので，二週間ぶりの研究室セミナーでした．今日の文献紹介の担当は化学生態学研究室修士二年の吉田さんで，紹介した論文は Xia, J., Yamaji, N. & Ma, J. F. A plasma membrane-localized small peptide is involved in rice aluminum tolerance. Plant J 76, 345-355 (2013) でした．イネのアルミニウム耐性に関与する原形質膜局在性ペプチドについての論文です．
2013年10月18日金曜日
今週の輪読
今週の水曜日は台風 26 号の関東地方への接近で茨城大学は全学休講となり，研究室セミナーも開催しませんでした．
今週の輪読では下記の論文を取り上げました．
410. Wasternack, C. Action of jasmonates in plant stress responses and development – Applied aspects. Biotechnol. Adv. in press.
411. Fu, L., Yu, X., and An, C. (2013). Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. Plant Physiol. Biochem. 73: 202–210.
412. Kleine, S., and Müller, C. Differences in shoot and root terpenoid profiles and plant responses to fertilisation in Tanacetum vulgare. Phytochemistry in press.
413. Di Ferdinando, M., Brunetti, C., Agati, G., and Tattini, M. Multiple functions of polyphenols in plants inhabiting unfavorable Mediterranean areas. Environ. Exp. Bot. in press.
今週の輪読では下記の論文を取り上げました．
410. Wasternack, C. Action of jasmonates in plant stress responses and development – Applied aspects. Biotechnol. Adv. in press.
411. Fu, L., Yu, X., and An, C. (2013). Overexpression of constitutively active OsCPK10 increases Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea. Plant Physiol. Biochem. 73: 202–210.
412. Kleine, S., and Müller, C. Differences in shoot and root terpenoid profiles and plant responses to fertilisation in Tanacetum vulgare. Phytochemistry in press.
413. Di Ferdinando, M., Brunetti, C., Agati, G., and Tattini, M. Multiple functions of polyphenols in plants inhabiting unfavorable Mediterranean areas. Environ. Exp. Bot. in press.
2013年10月15日火曜日
研究室セミナー & 先週の輪読
先週の 10/9 から後期の文献紹介が開始しました．先週の文献紹介の担当は博士一年の堀江君で，紹介した論文は Sugiura, Y., Zaima, N., Setou, M., Ito, S. & Yao, I. Visualization of acetylcholine distribution in central nervous system tissue sections by tandem imaging mass spectrometry. Anal Bioanal Chem 403, 1851-1861 (2012) でした．中枢神経系組織におけるアセチルコリン局在性の MALDI-MS/MS イメージングについての論文です．
先週の輪読では下記の論文を取り上げました．
405. Liang, Y. et al. (2013). Nonlegumes respond to rhizobial Nod factors by suppressing the innate immune response. Science 341: 1384–1387.
406. Widemann, E. et al. The amido-hydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxy-jasmonic acid upon wounding in Arabidopsis leaves. J. Biol. Chem. in press.
407. Meslet-Cladière, L. et al. (2013). Structure/function analysis of a type III polyketide synthase in the brown alga Ectocarpus siliculosus reveals a biochemical pathway in phlorotannin monomer biosynthesis. Plant Cell 25: 3089–3103.
408. Khalil, M.N.A. et al. Biosynthesis of the biphenyl phytoalexin aucuparin in Sorbus aucuparia cell cultures treated with Venturia inaequalis. Phytochemistry in press.
409. Liu, M., Li, X., Liu, Y., and Cao, B. (2013). Regulation of flavanone 3-hydroxylase gene involved in the flavonoid biosynthesis pathway in response to UV-B radiation and drought stress in the desert plant, Reaumuria soongorica. Plant Physiol. Biochem. 73: 161–167.
先週の輪読では下記の論文を取り上げました．
405. Liang, Y. et al. (2013). Nonlegumes respond to rhizobial Nod factors by suppressing the innate immune response. Science 341: 1384–1387.
406. Widemann, E. et al. The amido-hydrolases IAR3 and ILL6 contribute to jasmonoyl-isoleucine hormone turnover and generate 12-hydroxy-jasmonic acid upon wounding in Arabidopsis leaves. J. Biol. Chem. in press.
407. Meslet-Cladière, L. et al. (2013). Structure/function analysis of a type III polyketide synthase in the brown alga Ectocarpus siliculosus reveals a biochemical pathway in phlorotannin monomer biosynthesis. Plant Cell 25: 3089–3103.
408. Khalil, M.N.A. et al. Biosynthesis of the biphenyl phytoalexin aucuparin in Sorbus aucuparia cell cultures treated with Venturia inaequalis. Phytochemistry in press.
409. Liu, M., Li, X., Liu, Y., and Cao, B. (2013). Regulation of flavanone 3-hydroxylase gene involved in the flavonoid biosynthesis pathway in response to UV-B radiation and drought stress in the desert plant, Reaumuria soongorica. Plant Physiol. Biochem. 73: 161–167.
2013年10月5日土曜日
市立銚子高校模擬授業，研究室旅行，中間発表 & 先週・今週の輪読
先週はブログ更新をさぼってしまいましたが，この二週間はいろいろな出来事がありましたので，まとめて書きます．
9/26（木）は長谷川が銚子市立銚子高等学校での模擬授業を実施してきました．二年生が対象で「はじめての農薬学」というタイトルで農薬の定義や安全性などについての 60 分の講義を 2 回行いました．参加者は計 30 名程度でした．この日は台風の接近の影響で強風だったので，成田線のダイヤが大幅に乱れて，特に帰りは大変でした．
9/27（金）は生物制御化学研究室・化学生態学研究室合同での研究室旅行が開催されました．旅行といっても近場の日帰りレクリエーションでした．午前中は石岡のミッドクリークフィッシングエリアという釣り堀でルアーを使ったニジマス釣りをしました．ルアーで釣るのはなかなか大変で，しかも竿が 4 本だけで全員で同時には釣ることができなかったため，釣果は戸嶋先生 2，鈴木先生 1，堀江君 1 の合計 4 匹とやや寂しいものでした．釣った魚は焼いて 3 人で一匹の割合で食べました．これだけでは食事にならなかったので，併設のレストハウスで昼食を取りました．釣りの後は土浦のパニックボウルつくばでボーリングを 2 ゲーム行いました．これも戸嶋先生が圧倒的に一番良いスコアでした．最後は阿見に帰ってきて，食いしん坊炎で飲み会でした．
10/2（水）には研究室の中間発表会が開催されました．なかなか研究計画通りには進んでいませんが，卒論や修論をまとめるには残り時間はせいぜい 4 ヶ月程度とあまり残っているわけではないので，一層の奮起を期待しています．
先週と今週の輪読では下記の論文を取り上げました．
397. Durango, D., Pulgarin, N., Echeverri, F., Escobar, G., and Quiñones, W. (2013). Effect of salicylic acid and structurally related compounds in the accumulation of phytoalexins in cotyledons of common bean (Phaseolus vulgaris L.) cultivars. Molecules 18: 10609–10628.
398. Park, H.L., Lee, S.-W., Jung, K.-H., Hahn, T.-R., and Cho, M.-H. Transcriptomic analysis of UV-treated rice leaves reveals UV-induced phytoalexin biosynthetic pathways and their regulatory networks in rice. Phytochemistry in press.
399. Chung, S.H. et al. (2013). Herbivore exploits orally secreted bacteria to suppress plant defenses. Proc. Natl. Acad. Sci. USA 110: 15728–15733.
400. Quinet, M. et al. (2010). Putrescine differently influences the effect of salt stress on polyamine metabolism and ethylene synthesis in rice cultivars differing in salt resistance. J. Exp. Bot. 61: 2719–2733.
401. Cheng, X. et al. A rice lectin receptor-like kinase that is involved in innate immune responses also contributes to seed germination. Plant J. in press.
402. Rahman, M.M., El-Aty, A.M.A., and Shim, J.-H. Matrix enhancement effect: a blessing or a curse for gas chromatography: a review. Anal. Chim. Acta in press.
403. Kano, A. et al. The rare sugar D-allose acts as a triggering molecule of rice defence via ROS generation. J. Exp. Bot. in press.
404. Yokotani, N. et al. OsWRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance. J. Exp. Bot. in press.
9/26（木）は長谷川が銚子市立銚子高等学校での模擬授業を実施してきました．二年生が対象で「はじめての農薬学」というタイトルで農薬の定義や安全性などについての 60 分の講義を 2 回行いました．参加者は計 30 名程度でした．この日は台風の接近の影響で強風だったので，成田線のダイヤが大幅に乱れて，特に帰りは大変でした．
9/27（金）は生物制御化学研究室・化学生態学研究室合同での研究室旅行が開催されました．旅行といっても近場の日帰りレクリエーションでした．午前中は石岡のミッドクリークフィッシングエリアという釣り堀でルアーを使ったニジマス釣りをしました．ルアーで釣るのはなかなか大変で，しかも竿が 4 本だけで全員で同時には釣ることができなかったため，釣果は戸嶋先生 2，鈴木先生 1，堀江君 1 の合計 4 匹とやや寂しいものでした．釣った魚は焼いて 3 人で一匹の割合で食べました．これだけでは食事にならなかったので，併設のレストハウスで昼食を取りました．釣りの後は土浦のパニックボウルつくばでボーリングを 2 ゲーム行いました．これも戸嶋先生が圧倒的に一番良いスコアでした．最後は阿見に帰ってきて，食いしん坊炎で飲み会でした．
10/2（水）には研究室の中間発表会が開催されました．なかなか研究計画通りには進んでいませんが，卒論や修論をまとめるには残り時間はせいぜい 4 ヶ月程度とあまり残っているわけではないので，一層の奮起を期待しています．
先週と今週の輪読では下記の論文を取り上げました．
397. Durango, D., Pulgarin, N., Echeverri, F., Escobar, G., and Quiñones, W. (2013). Effect of salicylic acid and structurally related compounds in the accumulation of phytoalexins in cotyledons of common bean (Phaseolus vulgaris L.) cultivars. Molecules 18: 10609–10628.
398. Park, H.L., Lee, S.-W., Jung, K.-H., Hahn, T.-R., and Cho, M.-H. Transcriptomic analysis of UV-treated rice leaves reveals UV-induced phytoalexin biosynthetic pathways and their regulatory networks in rice. Phytochemistry in press.
399. Chung, S.H. et al. (2013). Herbivore exploits orally secreted bacteria to suppress plant defenses. Proc. Natl. Acad. Sci. USA 110: 15728–15733.
400. Quinet, M. et al. (2010). Putrescine differently influences the effect of salt stress on polyamine metabolism and ethylene synthesis in rice cultivars differing in salt resistance. J. Exp. Bot. 61: 2719–2733.
401. Cheng, X. et al. A rice lectin receptor-like kinase that is involved in innate immune responses also contributes to seed germination. Plant J. in press.
402. Rahman, M.M., El-Aty, A.M.A., and Shim, J.-H. Matrix enhancement effect: a blessing or a curse for gas chromatography: a review. Anal. Chim. Acta in press.
403. Kano, A. et al. The rare sugar D-allose acts as a triggering molecule of rice defence via ROS generation. J. Exp. Bot. in press.
404. Yokotani, N. et al. OsWRKY76 is a rice transcriptional repressor playing opposite roles in blast disease resistance and cold stress tolerance. J. Exp. Bot. in press.
2013年9月21日土曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
393. Scala, A., Allmann, S., Mirabella, R., Haring, M.A., and Schuurink, R.C. (2013). Green leaf volatiles: a plant’s multifunctional weapon against herbivores and pathogens. Int. J. Mol. Sci. 14: 17781–17811.
394. Vanholme, R. et al. (2013). Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. Science 341: 1103–1106.
395. Ye, M. et al. (2013). Priming of jasmonate-mediated antiherbivore defense responses in rice by silicon. Proc. Natl. Acad. Sci. USA 110: E3631–E3639.
396. Dafoe, N.J. et al. (2013). European corn borer (Ostrinia nubilalis) induced responses enhance susceptibility in maize. PLoS ONE 8: e73394.
393. Scala, A., Allmann, S., Mirabella, R., Haring, M.A., and Schuurink, R.C. (2013). Green leaf volatiles: a plant’s multifunctional weapon against herbivores and pathogens. Int. J. Mol. Sci. 14: 17781–17811.
394. Vanholme, R. et al. (2013). Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in Arabidopsis. Science 341: 1103–1106.
395. Ye, M. et al. (2013). Priming of jasmonate-mediated antiherbivore defense responses in rice by silicon. Proc. Natl. Acad. Sci. USA 110: E3631–E3639.
396. Dafoe, N.J. et al. (2013). European corn borer (Ostrinia nubilalis) induced responses enhance susceptibility in maize. PLoS ONE 8: e73394.
2013年9月13日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
388. Meldau, D.G. et al. (2013). Dimethyl disulfide produced by the naturally associated bacterium Bacillus sp B55 promotes Nicotiana attenuata growth by enhancing sulfur nutrition. Plant Cell 25: 2731–2747.
389. Aisyah, S., Gruppen, H., Madzora, B., and Vincken, J.-P. (2013). Modulation of isoflavonoid composition of Rhizopus oryzae elicited soybean (Glycine max) seedlings by light and wounding. J. Agric. Food Chem. 61: 8657–8667.
390. Schultz, J.C., Appel, H.M., Ferrieri, A., and Arnold, T.M. (2013). Flexible resource allocation during plant defense responses. Front. Plant Sci. 4: 324.
391. Pastor, V., Luna, E., Mauch-Mani, B., Ton, J., and Flors, V. (2013). Primed plants do not forget. Environ. Exp. Bot. 94: 46–56.
392. Chen, K., and Arora, R. (2013). Priming memory invokes seed stress-tolerance. Environ. Exp. Bot. 94: 33–45.
388. Meldau, D.G. et al. (2013). Dimethyl disulfide produced by the naturally associated bacterium Bacillus sp B55 promotes Nicotiana attenuata growth by enhancing sulfur nutrition. Plant Cell 25: 2731–2747.
389. Aisyah, S., Gruppen, H., Madzora, B., and Vincken, J.-P. (2013). Modulation of isoflavonoid composition of Rhizopus oryzae elicited soybean (Glycine max) seedlings by light and wounding. J. Agric. Food Chem. 61: 8657–8667.
390. Schultz, J.C., Appel, H.M., Ferrieri, A., and Arnold, T.M. (2013). Flexible resource allocation during plant defense responses. Front. Plant Sci. 4: 324.
391. Pastor, V., Luna, E., Mauch-Mani, B., Ton, J., and Flors, V. (2013). Primed plants do not forget. Environ. Exp. Bot. 94: 46–56.
392. Chen, K., and Arora, R. (2013). Priming memory invokes seed stress-tolerance. Environ. Exp. Bot. 94: 33–45.
2013年9月7日土曜日
停電 & 今週の輪読
明日は年に一度の点検のための停電が予定されています．停電のときには質量分析計を停止して真空を解除する必要がありますので，ついでに LC/MS のメンテナンスを行いました．
今週の輪読では下記の論文を取り上げました．
383. Fernandez, J., Yang, K.T., Cornwell, K.M., Wright, J.D., and Wilson, R.A. (2013). Growth in rice cells requires de novo purine biosynthesis by the blast fungus Magnaporthe oryzae. Sci. Rep. 3: 2398.
384. Mousavi, S.A.R., Chauvin, A., Pascaud, F., Kellenberger, S., and Farmer, E.E. (2013). GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling. Nature 500: 422–426.
385. Manzoor, H. et al. Involvement of the glutamate receptor AtGLR3.3 in plant defense signaling and resistance to Hyaloperonospora arabidopsidis. Plant J. in press.
386. Widiastuti, A. et al. (2013). Heat shock-induced resistance in strawberry against crown rot fungus Colletotrichum gloeosporioides. Physiol. Mol. Plant Pathol. 84: 86–91.
387. Altshuler, O. et al. Enantioselective effects of (+)- and (−)-citronellal on animal and plant microtubules. J. Nat. Prod. in press.
今週の輪読では下記の論文を取り上げました．
383. Fernandez, J., Yang, K.T., Cornwell, K.M., Wright, J.D., and Wilson, R.A. (2013). Growth in rice cells requires de novo purine biosynthesis by the blast fungus Magnaporthe oryzae. Sci. Rep. 3: 2398.
384. Mousavi, S.A.R., Chauvin, A., Pascaud, F., Kellenberger, S., and Farmer, E.E. (2013). GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling. Nature 500: 422–426.
385. Manzoor, H. et al. Involvement of the glutamate receptor AtGLR3.3 in plant defense signaling and resistance to Hyaloperonospora arabidopsidis. Plant J. in press.
386. Widiastuti, A. et al. (2013). Heat shock-induced resistance in strawberry against crown rot fungus Colletotrichum gloeosporioides. Physiol. Mol. Plant Pathol. 84: 86–91.
387. Altshuler, O. et al. Enantioselective effects of (+)- and (−)-citronellal on animal and plant microtubules. J. Nat. Prod. in press.
2013年8月31日土曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
378. Lakshman, D.K., Natarajan, S., Mandal, S., and Mitra, A. Lactoferrin-derived resistance against plant pathogens in transgenic plants. J. Agric. Food Chem. in press.
379. Poschenrieder, C., Cabot, C., Martos, S., Gallego, B., and Barceló, J. (2013). Do toxic ions induce hormesis in plants? Plant Sci. 212: 15–25.
380. Okamoto, M. et al. (2013). Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance. Proc. Natl. Acad. Sci. USA 110: 12132–12137.
381. Schweizer, F. et al. Arabidopsis basic helix-loop-helix transcription factors MYC2, MYC3, and MYC4 regulate glucosinolate biosynthesis, insect performance, and feeding behavior. Plant Cell in press.
382. Willett, C.D. et al. (2013). Identification of an atrazine-degrading benzoxazinoid in eastern gamagrass (Tripsacum dactyloides). J. Agric. Food Chem. 61: 8026–8033.
378. Lakshman, D.K., Natarajan, S., Mandal, S., and Mitra, A. Lactoferrin-derived resistance against plant pathogens in transgenic plants. J. Agric. Food Chem. in press.
379. Poschenrieder, C., Cabot, C., Martos, S., Gallego, B., and Barceló, J. (2013). Do toxic ions induce hormesis in plants? Plant Sci. 212: 15–25.
380. Okamoto, M. et al. (2013). Activation of dimeric ABA receptors elicits guard cell closure, ABA-regulated gene expression, and drought tolerance. Proc. Natl. Acad. Sci. USA 110: 12132–12137.
381. Schweizer, F. et al. Arabidopsis basic helix-loop-helix transcription factors MYC2, MYC3, and MYC4 regulate glucosinolate biosynthesis, insect performance, and feeding behavior. Plant Cell in press.
382. Willett, C.D. et al. (2013). Identification of an atrazine-degrading benzoxazinoid in eastern gamagrass (Tripsacum dactyloides). J. Agric. Food Chem. 61: 8026–8033.
2013年8月24日土曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
373. Xin, P., Yan, J., Fan, J., Chu, J., and Yan, C. (2013). An improved simplified high-sensitivity quantification method for determining brassinosteroids in different tissues of rice and Arabidopsis. Plant Physiol. 162: 2056–2066.
374. García, I., Rosas, T., Bejarano, E.R., Gotor, C., and Romero, L.C. (2013). Transient transcriptional regulation of the CYS-C1 gene and cyanide accumulation upon pathogen infection in the plant immune response. Plant Physiol. 162: 2015–2027.
375. Hsu, C.-C. et al. (2013). Real-time metabolomics on living microorganisms using ambient electrospray ionization flow-probe. Anal. Chem. 85: 7014–7018.
376. Hemalatha, R.G., and Pradeep, T. (2013). Understanding the molecular signatures in leaves and flowers by desorption electrospray ionization mass spectrometry (DESI MS) imaging. J. Agric. Food Chem. 61: 7477–7487.
377. Corona-Castañeda, B., Chérigo, L., Fragoso-Serrano, M., Gibbons, S., and Pereda-Miranda, R. Modulators of antibiotic activity from Ipomoea murucoides. Phytochemistry in press.
373. Xin, P., Yan, J., Fan, J., Chu, J., and Yan, C. (2013). An improved simplified high-sensitivity quantification method for determining brassinosteroids in different tissues of rice and Arabidopsis. Plant Physiol. 162: 2056–2066.
374. García, I., Rosas, T., Bejarano, E.R., Gotor, C., and Romero, L.C. (2013). Transient transcriptional regulation of the CYS-C1 gene and cyanide accumulation upon pathogen infection in the plant immune response. Plant Physiol. 162: 2015–2027.
375. Hsu, C.-C. et al. (2013). Real-time metabolomics on living microorganisms using ambient electrospray ionization flow-probe. Anal. Chem. 85: 7014–7018.
376. Hemalatha, R.G., and Pradeep, T. (2013). Understanding the molecular signatures in leaves and flowers by desorption electrospray ionization mass spectrometry (DESI MS) imaging. J. Agric. Food Chem. 61: 7477–7487.
377. Corona-Castañeda, B., Chérigo, L., Fragoso-Serrano, M., Gibbons, S., and Pereda-Miranda, R. Modulators of antibiotic activity from Ipomoea murucoides. Phytochemistry in press.
2013年8月14日水曜日
夏季全学一斉休業 & 今週の輪読
茨城大学では 8/14-16 が全学一斉休業日に指定されているので，研究室もこの期間は夏休みになります．研究室再開は 8/19 からです．
今週の輪読では下記の論文を取り上げました．
371. Boubakri, H. et al. Methionine elicits H2O2 generation and defense gene expression in grapevine and reduces Plasmopara viticola infection. J. Plant Physiol. in press.
372. Itkin, M. et al. (2013). Biosynthesis of antinutritional alkaloids in Solanaceous crops is mediated by clustered genes. Science 341: 175–179.
今週の輪読では下記の論文を取り上げました．
371. Boubakri, H. et al. Methionine elicits H2O2 generation and defense gene expression in grapevine and reduces Plasmopara viticola infection. J. Plant Physiol. in press.
372. Itkin, M. et al. (2013). Biosynthesis of antinutritional alkaloids in Solanaceous crops is mediated by clustered genes. Science 341: 175–179.
2013年8月10日土曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．学部の三年生までは今週で試験も終わって夏休み突入ですが，研究室はまだ営業中です．
367. Flores, G., Blanch, G.P., and Ruiz del Castillo, M.L. (2013). Isolation of the four methyl jasmonate stereoisomers and their effects on selected chiral volatile compounds in red raspberries. Food Chem. 141: 2982–2987.
368. Gruner, K., Griebel, T., Návarová, H., Attaran, E., and Zeier, J. (2013). Reprogramming of plants during systemic acquired resistance. Front. Plant Sci. 4: 252.
369. Meihls, L.N. et al. (2013). Natural variation in maize aphid resistance is associated with 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside methyltransferase activity. Plant Cell 25: 2341–2355.
370. Stallforth, P. et al. A bacterial symbiont is converted from an inedible producer of beneficial molecules into food by a single mutation in the gacA gene. Proc. Natl. Acad. Sci. USA in press.
367. Flores, G., Blanch, G.P., and Ruiz del Castillo, M.L. (2013). Isolation of the four methyl jasmonate stereoisomers and their effects on selected chiral volatile compounds in red raspberries. Food Chem. 141: 2982–2987.
368. Gruner, K., Griebel, T., Návarová, H., Attaran, E., and Zeier, J. (2013). Reprogramming of plants during systemic acquired resistance. Front. Plant Sci. 4: 252.
369. Meihls, L.N. et al. (2013). Natural variation in maize aphid resistance is associated with 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one glucoside methyltransferase activity. Plant Cell 25: 2341–2355.
370. Stallforth, P. et al. A bacterial symbiont is converted from an inedible producer of beneficial molecules into food by a single mutation in the gacA gene. Proc. Natl. Acad. Sci. USA in press.
2013年8月3日土曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
362. Ueda, Y., Uehara, N., Sasaki, H., Kobayashi, K., and Yamakawa, T. (2013). Impacts of acute ozone stress on superoxide dismutase (SOD) expression and reactive oxygen species (ROS) formation in rice leaves. Plant Physiol. Biochem. 70: 396–402.
363. Park, S.-Y. et al. (2013). Metabolite profiling approach reveals the interface of primary and secondary metabolism in colored cauliflowers (Brassica oleracea L. ssp. botrytis). J. Agric. Food Chem. 61: 6999–7007.
364. Shimizu, T. et al. (2013). OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice. Biosci. Biotechnol. Biochem. 77: 1556–1564.
365. Jeandet, P., Clément, C., Courot, E., and Cordelier, S. (2013). Modulation of phytoalexin biosynthesis in engineered plants for disease resistance. Int. J. Mol. Sci. 14: 14136–14170.
366. Sasaki-Sekimoto, Y. et al. bHLH transcription factors JA-ASSOCIATED MYC2-LIKE 1, JAM2 and JAM3 are negative regulators of jasmonate responses in Arabidopsis. Plant Physiol. in press.
362. Ueda, Y., Uehara, N., Sasaki, H., Kobayashi, K., and Yamakawa, T. (2013). Impacts of acute ozone stress on superoxide dismutase (SOD) expression and reactive oxygen species (ROS) formation in rice leaves. Plant Physiol. Biochem. 70: 396–402.
363. Park, S.-Y. et al. (2013). Metabolite profiling approach reveals the interface of primary and secondary metabolism in colored cauliflowers (Brassica oleracea L. ssp. botrytis). J. Agric. Food Chem. 61: 6999–7007.
364. Shimizu, T. et al. (2013). OsJAR1 contributes mainly to biosynthesis of the stress-induced jasmonoyl-isoleucine involved in defense responses in rice. Biosci. Biotechnol. Biochem. 77: 1556–1564.
365. Jeandet, P., Clément, C., Courot, E., and Cordelier, S. (2013). Modulation of phytoalexin biosynthesis in engineered plants for disease resistance. Int. J. Mol. Sci. 14: 14136–14170.
366. Sasaki-Sekimoto, Y. et al. bHLH transcription factors JA-ASSOCIATED MYC2-LIKE 1, JAM2 and JAM3 are negative regulators of jasmonate responses in Arabidopsis. Plant Physiol. in press.
2013年7月27日土曜日
研究室セミナー & 先々週・先週・今週の輪読
三週間ほどブログの更新をさぼってしまいました．7/10 の研究室セミナーの文献紹介の担当は四年生の五十嵐さんで，紹介した論文は Bon, D. J.-Y. D., Banwell, M. G., Ward, J. S. & Willis, A. C. From toluene to triquinanes: formal total syntheses of the sesquiterpenoid natural products (−)-hypnophilin and (−)-coriolin. Tetrahedron 69, 1363-1368 (2013) でした．抗トリパノソーマ活性などを有する triquinane 型セスキテルペン化合物の形式的全合成についての論文です．前期の研究室セミナーでの文献紹介はこれで終了し，後期は 10 月から再開です．
先々週から今週にかけての輪読では下記の論文を取り上げました．
349. Zhang, C. et al. (2013). Crosstalk between the circadian clock and innate immunity in Arabidopsis. PLoS Pathogens 9: e1003370.
350. Tytgat, T.O.G. et al. (2013). Plants know where it hurts: root and shoot jasmonic acid induction elicit differential responses in Brassica oleracea. PLoS ONE 8: e65502.
351. Dinh, S.T., Baldwin, I.T., and Galis, I. The HERBIVORE ELICITOR-REGULATED1 (HER1) gene enhances abscisic acid levels and defenses against herbivores in Nicotiana attenuata plants. Plant Physiol. in press.
352. Hamada, A.M., and Jonsson, L.M.V. Thiamine treatments alleviate aphid infestations in barley and pea. Phytochemistry in press.
353. Wu, Y., Wang, Q., Hillwig, M.L., and Peters, R.J. Picking sides: Distinct roles for CYP76M6 and -8 in rice oryzalexin biosynthesis. Biochem. J. in press.
354. Saikkonen, K., Gundel, P.E., and Helander, M. Chemical ecology mediated by fungal endophytes in grasses. J. Chem. Ecol. in press.
355. Song, Y.Y. et al. Priming of anti-herbivore defense in tomato by arbuscular mycorrhizal fungus and involvement of the jasmonate pathway. J. Chem. Ecol. in press.
356. Boone, C.K. et al. Bacteria associated with a tree-killing insect reduce concentrations of plant defense compounds. J. Chem. Ecol. in press.
357. Oluwafemi, S. et al. (2013). Priming of production in maize of volatile organic defence compounds by the natural plant activator cis-jasmone. PLoS ONE 8: e62299.
358. Hammerbacher, A. et al. (2013). A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce. Plant Physiol. 162: 1324–1336.
359. Mur, L.A.J., Prats, E., Pierre, S., Hall, M.A., and Hebelstrup, K.H. (2013). Integrating nitric oxide into salicylic acid and jasmonic acid/ ethylene plant defence pathways. Front. Plant Sci. 4: 215.
360. Xu, J., Audenaert, K., Hofte, M., and De Vleesschauwer, D. (2013). Abscisic acid promotes susceptibility to the rice leaf blight pathogen Xanthomonas oryzae pv oryzae by suppressing salicylic acid-mediated defenses. PLoS ONE 8: e67413.
361. Møldrup, M.E., Salomonsen, B., Geu-Flores, F., Olsen, C.E., and Halkier, B.A. de novo genetic engineering of the camalexin biosynthetic pathway. J. Biotechnol. in press.
先々週から今週にかけての輪読では下記の論文を取り上げました．
349. Zhang, C. et al. (2013). Crosstalk between the circadian clock and innate immunity in Arabidopsis. PLoS Pathogens 9: e1003370.
350. Tytgat, T.O.G. et al. (2013). Plants know where it hurts: root and shoot jasmonic acid induction elicit differential responses in Brassica oleracea. PLoS ONE 8: e65502.
351. Dinh, S.T., Baldwin, I.T., and Galis, I. The HERBIVORE ELICITOR-REGULATED1 (HER1) gene enhances abscisic acid levels and defenses against herbivores in Nicotiana attenuata plants. Plant Physiol. in press.
352. Hamada, A.M., and Jonsson, L.M.V. Thiamine treatments alleviate aphid infestations in barley and pea. Phytochemistry in press.
353. Wu, Y., Wang, Q., Hillwig, M.L., and Peters, R.J. Picking sides: Distinct roles for CYP76M6 and -8 in rice oryzalexin biosynthesis. Biochem. J. in press.
354. Saikkonen, K., Gundel, P.E., and Helander, M. Chemical ecology mediated by fungal endophytes in grasses. J. Chem. Ecol. in press.
355. Song, Y.Y. et al. Priming of anti-herbivore defense in tomato by arbuscular mycorrhizal fungus and involvement of the jasmonate pathway. J. Chem. Ecol. in press.
356. Boone, C.K. et al. Bacteria associated with a tree-killing insect reduce concentrations of plant defense compounds. J. Chem. Ecol. in press.
357. Oluwafemi, S. et al. (2013). Priming of production in maize of volatile organic defence compounds by the natural plant activator cis-jasmone. PLoS ONE 8: e62299.
358. Hammerbacher, A. et al. (2013). A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce. Plant Physiol. 162: 1324–1336.
359. Mur, L.A.J., Prats, E., Pierre, S., Hall, M.A., and Hebelstrup, K.H. (2013). Integrating nitric oxide into salicylic acid and jasmonic acid/ ethylene plant defence pathways. Front. Plant Sci. 4: 215.
360. Xu, J., Audenaert, K., Hofte, M., and De Vleesschauwer, D. (2013). Abscisic acid promotes susceptibility to the rice leaf blight pathogen Xanthomonas oryzae pv oryzae by suppressing salicylic acid-mediated defenses. PLoS ONE 8: e67413.
361. Møldrup, M.E., Salomonsen, B., Geu-Flores, F., Olsen, C.E., and Halkier, B.A. de novo genetic engineering of the camalexin biosynthetic pathway. J. Biotechnol. in press.
2013年7月5日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．月曜日は出席者が一名になってしまったので，中止しました．
345. Wang, H. et al. Flower color polymorphism in Iris lutescens (Iridaceae): Biochemical analyses in light of plant–insect interactions. Phytochemistry in press.
346. Chen, H. et al. (2013). Quality assessment of Japanese knotweed (Fallopia japonica) grown on Prince Edward Island as a source of resveratrol. J. Agric. Food Chem. 61: 6383–6392.
347. Gao, Y., Yu, Y., Hu, X., Cao, Y., and Wu, J. Imaging of jasmonic acid binding sites in tissue. Anal. Biochem. in press.
348. Denancé, N., Sánchez-Vallet, A., Goffner, D., and Molina, A. (2013). Disease resistance or growth: the role of plant hormones in balancing immune responses and fitness costs. Front. Plant Sci. 4: 155.
345. Wang, H. et al. Flower color polymorphism in Iris lutescens (Iridaceae): Biochemical analyses in light of plant–insect interactions. Phytochemistry in press.
346. Chen, H. et al. (2013). Quality assessment of Japanese knotweed (Fallopia japonica) grown on Prince Edward Island as a source of resveratrol. J. Agric. Food Chem. 61: 6383–6392.
347. Gao, Y., Yu, Y., Hu, X., Cao, Y., and Wu, J. Imaging of jasmonic acid binding sites in tissue. Anal. Biochem. in press.
348. Denancé, N., Sánchez-Vallet, A., Goffner, D., and Molina, A. (2013). Disease resistance or growth: the role of plant hormones in balancing immune responses and fitness costs. Front. Plant Sci. 4: 155.
2013年6月29日土曜日
研究室セミナー & 今週の輪読
今週水曜日の研究室セミナーの文献紹介の担当は化学生態学研究室四年生の武井さんで，紹介した論文は Montillet, J.-L. et al. An abscisic acid-independent oxylipin pathway controls stomatal closure and immune defense in Arabidopsis. PLoS Biol. 11, e1001513 (2013) でした．
病原細菌に対する抵抗性反応として起こる気孔の閉孔にはアブシジン酸には依存しないオキシリピンのシグナル伝達経路が関与していることを示した報告です．
今週の輪読では下記の論文を取り上げました．
340. Newman, M.-A., Sundelin, T., Nielsen, J.T., and Erbs, G. (2013). MAMP (Microbe-Associated Molecular Pattern) triggered immunity in Plants. Front. Plant Sci. 4: 139.
341. Matsuba, Y. et al. Evolution of a complex locus for terpene biosynthesis in Solanum. Plant Cell in press.
342. Mikulic-Petkovsek, M. et al. (2013). Alteration of the content of primary and secondary metabolites in strawberry fruit by Colletotrichum nymphaeae infection. J. Agric. Food Chem. 61: 5987–5995.
343. Tocci, N. et al. (2013). Bioassay-guided fractionation of extracts from Hypericum perforatum in vitro roots treated with carboxymethylchitosans and determination of antifungal activity against human fungal pathogens. Plant Physiol. Biochem. 70: 342–347.
344. Wilcock, B.C., Endo, M.M., Uno, B.E., and Burke, M.D. (2013). C2′-OH of amphotericin B plays an important role in binding the primary sterol of human cells but not yeast cells. J. Am. Chem. Soc. 135: 8488–8491.
病原細菌に対する抵抗性反応として起こる気孔の閉孔にはアブシジン酸には依存しないオキシリピンのシグナル伝達経路が関与していることを示した報告です．
今週の輪読では下記の論文を取り上げました．
340. Newman, M.-A., Sundelin, T., Nielsen, J.T., and Erbs, G. (2013). MAMP (Microbe-Associated Molecular Pattern) triggered immunity in Plants. Front. Plant Sci. 4: 139.
341. Matsuba, Y. et al. Evolution of a complex locus for terpene biosynthesis in Solanum. Plant Cell in press.
342. Mikulic-Petkovsek, M. et al. (2013). Alteration of the content of primary and secondary metabolites in strawberry fruit by Colletotrichum nymphaeae infection. J. Agric. Food Chem. 61: 5987–5995.
343. Tocci, N. et al. (2013). Bioassay-guided fractionation of extracts from Hypericum perforatum in vitro roots treated with carboxymethylchitosans and determination of antifungal activity against human fungal pathogens. Plant Physiol. Biochem. 70: 342–347.
344. Wilcock, B.C., Endo, M.M., Uno, B.E., and Burke, M.D. (2013). C2′-OH of amphotericin B plays an important role in binding the primary sterol of human cells but not yeast cells. J. Am. Chem. Soc. 135: 8488–8491.
2013年6月21日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
335. Ghosh, S., Indukuri, K., Bondalapati, S., Saikia, A.K., and Rangan, L. (2013). Unveiling the mode of action of antibacterial labdane diterpenes from Alpinia nigra (Gaertn.) B. L. Burtt seeds. Eur. J. Med. Chem. 66: 101–105.
336. Dai, Y., Witkamp, G.J., Verpoorte, R., and Choi, Y.H. Natural deep eutectic solvents as a new extraction media for phenolic metabolites in Carthamus tinctorius L. Anal. Chem. in press.
337. Yang, J.-O., Nakayama, N., Toda, K., Tebayashi, S., and Kim, C.-S. Elicitor(s) in Sogatella furcifera (Horváth) causing the Japanese rice plant (Oryza sativa L.) to induce the ovicidal substance, benzyl benzoate. Biosci. Biotechnol. Biochem. in press.
338. Moreira, X., et al. Inducibility of chemical defences by two chewing insect herbivores in pine trees is specific to targeted plant tissue, particular herbivore and defensive trait. Phytochemistry in press.
339. Silke, A. et al. (2013). Feeding-induced rearrangement of green leaf volatiles reduces moth oviposition. eLife 2: e00421.
335. Ghosh, S., Indukuri, K., Bondalapati, S., Saikia, A.K., and Rangan, L. (2013). Unveiling the mode of action of antibacterial labdane diterpenes from Alpinia nigra (Gaertn.) B. L. Burtt seeds. Eur. J. Med. Chem. 66: 101–105.
336. Dai, Y., Witkamp, G.J., Verpoorte, R., and Choi, Y.H. Natural deep eutectic solvents as a new extraction media for phenolic metabolites in Carthamus tinctorius L. Anal. Chem. in press.
337. Yang, J.-O., Nakayama, N., Toda, K., Tebayashi, S., and Kim, C.-S. Elicitor(s) in Sogatella furcifera (Horváth) causing the Japanese rice plant (Oryza sativa L.) to induce the ovicidal substance, benzyl benzoate. Biosci. Biotechnol. Biochem. in press.
338. Moreira, X., et al. Inducibility of chemical defences by two chewing insect herbivores in pine trees is specific to targeted plant tissue, particular herbivore and defensive trait. Phytochemistry in press.
339. Silke, A. et al. (2013). Feeding-induced rearrangement of green leaf volatiles reduces moth oviposition. eLife 2: e00421.
2013年6月19日水曜日
研究室セミナー
本日の研究室セミナーの文献紹介の担当は四年生の奥木さんで，紹介した論文は Quandt, E. M. et al. Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth. Biol. in press でした． 増殖にカフェインを必要とする大腸菌を遺伝子工学的に作成し，カフェインの定量や分解への応用を可能とした報告です．
2013年6月14日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
330. de Oteyza, D.G. et al. Direct imaging of covalent bond structure in single-molecule chemical reactions. Science in press.
331. Zerbe, P. et al. (2013). Gene discovery of modular diterpene metabolism in nonmodel systems. Plant Physiol. 162: 1073–1091.
332. Kim, J. et al. (2013). Structure-guided discovery of the metabolite carboxy-SAM that modulates tRNA function. Nature 498: 123–126.
333. Pereira-Caro, G. et al. Phytochemical profile of a Japanese black-purple rice. Food Chem. in press.
334. Selmar, D., and Kleinwächter, M. (2013). Stress enhances the synthesis of secondary plant products: the impact of stress-related over-reduction on the accumulation of natural products. Plant Cell Physiol. 54: 817–826.
330. de Oteyza, D.G. et al. Direct imaging of covalent bond structure in single-molecule chemical reactions. Science in press.
331. Zerbe, P. et al. (2013). Gene discovery of modular diterpene metabolism in nonmodel systems. Plant Physiol. 162: 1073–1091.
332. Kim, J. et al. (2013). Structure-guided discovery of the metabolite carboxy-SAM that modulates tRNA function. Nature 498: 123–126.
333. Pereira-Caro, G. et al. Phytochemical profile of a Japanese black-purple rice. Food Chem. in press.
334. Selmar, D., and Kleinwächter, M. (2013). Stress enhances the synthesis of secondary plant products: the impact of stress-related over-reduction on the accumulation of natural products. Plant Cell Physiol. 54: 817–826.
2013年6月12日水曜日
研究室セミナー
本日の研究室セミナーの文献紹介の担当は化学生態学研究室四年生の鈴木君で，紹介した論文は Nobusawa, T. et al. Synthesis of very-long-chain fatty acids in the epidermis controls plant organ growth by restricting cell proliferation. PLoS Biology 11, e1001531 (2013) でした． 植物表皮の超長鎖脂肪酸生合成が植物の生長を制限していることを報告した論文です．
2013年6月8日土曜日
下村伸君の論文が Biosci. Biotechnol. Biochem. 誌に掲載
2011 年 3 月に修士課程を修了した下村伸君が筆頭著者の論文が Biosci. Biotechnol. Biochem. 誌（日本農芸化学会の英文誌）のサイトでオンライン公開されました．
Shimomura, S., Oyama, S., Nakano, K., Hasegawa, M., and Toshima, H. (2013). Stereoselective synthesis of a promising flower-inducing KODA analog, (9R,12S,13R,15Z)-9-hydroxy-12,13-methylene-10-oxooctadec-15-enoic acid. Biosci. Biotechnol. Biochem. in press.
植物の花芽の形成は日長などの環境要因によって厳密に制御されています．花咲か爺さんの昔話にあるように，花芽形成を人間の意のままに自由に制御することができれば，その技術は農業生産や育種，園芸の現場などで大変役に立つはずです．
この論文は，アオウキクサから単離された花芽形成促進物質 KODA 類縁体の立体選択的合成を報告したものです．KODA は資生堂の横山らによって発見された化合物（こちらを参照）ですが，そのまま植物生長調節剤として用いるには不安定な化合物であるため，本研究室ではシクロプロパン環を導入した化学的に安定な類縁体を設計し，その合成方法について研究を行ってきました．本論文で報告した類縁体が花芽形成促進活性を有するかは今後の研究によって明らかにしなければいけませんが，有効な花芽形成促進物質となることが期待されています．
この論文は下村君だけでなく，今年修了した大山秀芳君と 2008 年修了の中野恭平君たちの修士論文研究成果の一部をまとめたものです．
Shimomura, S., Oyama, S., Nakano, K., Hasegawa, M., and Toshima, H. (2013). Stereoselective synthesis of a promising flower-inducing KODA analog, (9R,12S,13R,15Z)-9-hydroxy-12,13-methylene-10-oxooctadec-15-enoic acid. Biosci. Biotechnol. Biochem. in press.
植物の花芽の形成は日長などの環境要因によって厳密に制御されています．花咲か爺さんの昔話にあるように，花芽形成を人間の意のままに自由に制御することができれば，その技術は農業生産や育種，園芸の現場などで大変役に立つはずです．
この論文は，アオウキクサから単離された花芽形成促進物質 KODA 類縁体の立体選択的合成を報告したものです．KODA は資生堂の横山らによって発見された化合物（こちらを参照）ですが，そのまま植物生長調節剤として用いるには不安定な化合物であるため，本研究室ではシクロプロパン環を導入した化学的に安定な類縁体を設計し，その合成方法について研究を行ってきました．本論文で報告した類縁体が花芽形成促進活性を有するかは今後の研究によって明らかにしなければいけませんが，有効な花芽形成促進物質となることが期待されています．
KODA の化学構造（天然から単離された KODA は 1R:1S = 7:3 の混合物）
本論文で報告した KODA 類縁体
2013年6月7日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
325. Cesari, S. et al. (2013). The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding. Plant Cell 25: 1463–1481.
326. Chi, W.-C. et al. (2013). Autotoxicity mechanism of Oryza sativa: transcriptome response in rice roots exposed to ferulic acid. BMC Genomics 14: 351.
327. Bernstein, M.A., Sýkora, S., Peng, C., Barba, A., and Cobas, C. Optimization and automation of quantitative NMR data extraction. Anal. Chem. in press.
328. Pedras, M.S.C., and Abdoli, A. Metabolism of the phytoalexins camalexins, their bioisosteres and analogues in the plant pathogenic fungus Alternaria brassicicola. Bioorg. Med. Chem. in press.
329. Patterson, D., Schnell, M., and Doyle, J.M. (2013). Enantiomer-specific detection of chiral molecules via microwave spectroscopy. Nature 497: 475–477.
325. Cesari, S. et al. (2013). The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding. Plant Cell 25: 1463–1481.
326. Chi, W.-C. et al. (2013). Autotoxicity mechanism of Oryza sativa: transcriptome response in rice roots exposed to ferulic acid. BMC Genomics 14: 351.
327. Bernstein, M.A., Sýkora, S., Peng, C., Barba, A., and Cobas, C. Optimization and automation of quantitative NMR data extraction. Anal. Chem. in press.
328. Pedras, M.S.C., and Abdoli, A. Metabolism of the phytoalexins camalexins, their bioisosteres and analogues in the plant pathogenic fungus Alternaria brassicicola. Bioorg. Med. Chem. in press.
329. Patterson, D., Schnell, M., and Doyle, J.M. (2013). Enantiomer-specific detection of chiral molecules via microwave spectroscopy. Nature 497: 475–477.
2013年6月5日水曜日
研究室セミナー
本日の研究室セミナーの文献紹介の担当は四年生の渡辺さんで，紹介した論文は Kitada, S., Fujita, S., Okada, Y., Kim, S. & Chiba, K. Total synthesis of α-conotoxin MII using a soluble-tag-assisted method. Tetrahedron 69, 2555-2559 (2013) でした． 可溶性タグを用いた液相ペプチド合成法によるイモガイ由来の毒素 α-conotoxin MII の全合成に関する論文です．
2013年5月31日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
320. Mora-Pale, M., Sanchez-Rodriguez, S.P., Linhatrdt, R.J., Dordick, J.S., and Koffas, M.A.G. Metabolic engineering and in vitro biosynthesis of phytochemicals and non-natural analogues. Plant Sci. in press.
321. Wei, G.-J. et al. (2013). Identification of sinensetin metabolites in rat urine by an isotope-labeling method and ultrahigh-performance liquid chromatography–electrospray ionization mass spectrometry. J. Agric. Food Chem. 61: 5016–5021.
322. Fürstenberg-Hägg, J., Zagrobelny, M., and Bak, S. (2013). Plant defense against insect herbivores. Int. J. Mol. Sci. 14: 10242–10297.
323. Kato-Noguchi, H., and Peters, R.J. (2013). The role of momilactones in rice allelopathy. J. Chem. Ecol. 39: 175–185.
324. Fang, C. et al. (2013). Changes in rice allelopathy and rhizosphere microflora by inhibiting rice phenylalanine ammonia-lyase gene expression. J. Chem. Ecol. 39: 204–212.
320. Mora-Pale, M., Sanchez-Rodriguez, S.P., Linhatrdt, R.J., Dordick, J.S., and Koffas, M.A.G. Metabolic engineering and in vitro biosynthesis of phytochemicals and non-natural analogues. Plant Sci. in press.
321. Wei, G.-J. et al. (2013). Identification of sinensetin metabolites in rat urine by an isotope-labeling method and ultrahigh-performance liquid chromatography–electrospray ionization mass spectrometry. J. Agric. Food Chem. 61: 5016–5021.
322. Fürstenberg-Hägg, J., Zagrobelny, M., and Bak, S. (2013). Plant defense against insect herbivores. Int. J. Mol. Sci. 14: 10242–10297.
323. Kato-Noguchi, H., and Peters, R.J. (2013). The role of momilactones in rice allelopathy. J. Chem. Ecol. 39: 175–185.
324. Fang, C. et al. (2013). Changes in rice allelopathy and rhizosphere microflora by inhibiting rice phenylalanine ammonia-lyase gene expression. J. Chem. Ecol. 39: 204–212.
2013年5月26日日曜日
研究室セミナー & 先週の輪読
先週水曜日の研究室セミナーの文献紹介の担当は四年生の遠藤君で，紹介した論文は Kettles, G. J., Drurey, C., Schoonbeek, H.-j., Maule, A. J. & Hogenhout, S. A. Resistance of Arabidopsis thaliana to the green peach aphid, Myzus persicae, involves camalexin and is regulated by microRNAs. New Phytol. 198, 1178-1190 (2013) でした． モモアカアブラムシに対するシロイヌナズナの抵抗性にフィトアレキシンであるカマレキシンが関与していて，その制御にはマイクロ RNA が関わっていることを報告した論文です．
先週の輪読では下記の論文を取り上げました．
315. Tan, J. et al. (2013). A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin. Plant Physiol. 162: 86–95.
316. Schmidts, V. et al. (2013). RDC-based determination of the relative configuration of the fungicidal cyclopentenone 4,6-diacetylhygrophorone A12. J. Nat. Prod. 76: 839–844.
317. Wang, C., Bao, Y., Wang, Q., and Zhang, H. Introduction of the rice CYP714D1 gene into Populus inhibits expression of its homologous genes and promotes growth, biomass production, and xylem fibre length in transgenic trees. J. Exp. Bot. in press.
318. Jung, E.S. et al. Metabolite profiling of the short-term responses of rice leaves (Oryza sativa cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities. Plant Sci. in press.
319. Pajerowska-Mukhtar, K.M., Emerine, D.K., and Mukhtar, M.S. Tell me more: roles of NPRs in plant immunity. Trends Plant Sci. in press.
先週の輪読では下記の論文を取り上げました．
315. Tan, J. et al. (2013). A genetic and metabolic analysis revealed that cotton fiber cell development was retarded by flavonoid naringenin. Plant Physiol. 162: 86–95.
316. Schmidts, V. et al. (2013). RDC-based determination of the relative configuration of the fungicidal cyclopentenone 4,6-diacetylhygrophorone A12. J. Nat. Prod. 76: 839–844.
317. Wang, C., Bao, Y., Wang, Q., and Zhang, H. Introduction of the rice CYP714D1 gene into Populus inhibits expression of its homologous genes and promotes growth, biomass production, and xylem fibre length in transgenic trees. J. Exp. Bot. in press.
318. Jung, E.S. et al. Metabolite profiling of the short-term responses of rice leaves (Oryza sativa cv. Ilmi) cultivated under different LED lights and its correlations with antioxidant activities. Plant Sci. in press.
319. Pajerowska-Mukhtar, K.M., Emerine, D.K., and Mukhtar, M.S. Tell me more: roles of NPRs in plant immunity. Trends Plant Sci. in press.
2013年5月17日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
310. Belofsky, G. et al. Fungal ABC transporter-associated activity of isoflavonoids from the root extract of Dalea formosa. J. Nat. Prod. in press.
311. Scala, A. et al. (2013). E-2-hexenal promotes susceptibility to Pseudomonas syringae by activating jasmonic acid pathways in Arabidopsis. Front. Plant Sci. 4: 74.
312. Lai, D. et al. (2013). Cembranoids from the soft coral Sinularia rigida with antifouling activities. J. Agric. Food Chem. 61: 4585–4592.
313. Tokuda, M. et al. (2013). Phytohormones related to host plant manipulation by a gall-inducing leafhopper. PLoS ONE 8: e62350.
314. Wang, X. et al. (2013). Antifungal activity against plant pathogens of metabolites from the endophytic fungus Cladosporium cladosporioides. J. Agric. Food Chem. 61: 4551–4555.
310. Belofsky, G. et al. Fungal ABC transporter-associated activity of isoflavonoids from the root extract of Dalea formosa. J. Nat. Prod. in press.
311. Scala, A. et al. (2013). E-2-hexenal promotes susceptibility to Pseudomonas syringae by activating jasmonic acid pathways in Arabidopsis. Front. Plant Sci. 4: 74.
312. Lai, D. et al. (2013). Cembranoids from the soft coral Sinularia rigida with antifouling activities. J. Agric. Food Chem. 61: 4585–4592.
313. Tokuda, M. et al. (2013). Phytohormones related to host plant manipulation by a gall-inducing leafhopper. PLoS ONE 8: e62350.
314. Wang, X. et al. (2013). Antifungal activity against plant pathogens of metabolites from the endophytic fungus Cladosporium cladosporioides. J. Agric. Food Chem. 61: 4551–4555.
2013年5月15日水曜日
研究室セミナー
今日の研究室セミナーの文献紹介の担当は化学生態学研究室四年生の藤原さんで，紹介した論文は Motte, H. et al. Phenyl-adenine, identified in a LIGHT-DEPENDENT SHORT HYPOCOTYLS4-assisted chemical screen, is a potent compound for shoot regeneration through the inhibition of CYTOKININ OXIDASE/DEHYDROGENASE activity. Plant Physiol. 161, 1229-1241 (2013) でした．化合物ライブラリのスクリーニングから見いだしたシュート誘導物質フェニルアデニンについての報告です．
2013年5月10日金曜日
研究室セミナー & 今週の輪読
今週水曜日の研究室セミナーの文献紹介の担当は博士一年生の堀江君で，紹介した論文は Fujishima, S.-h., Yasui, R., Miki, T., Ojida, A. & Hamachi, I. Ligand-directed acyl imidazole chemistry for labeling of membrane-bound proteins on live cells. J. Am. Chem. Soc. 134, 3961-3964 (2012) でした．細胞内のタンパク質に特異的に結合できる新規標識法についての報告です．
先週水曜日の研究室セミナーの文献紹介の担当は修士一年生の濱名君で，紹介した論文は Li, B. et al. Visualizing metabolite distribution and enzymatic conversion in plant tissues by desorption electrospray ionization mass spectrometry imaging. Plant J. in press でした．植物組織内の青酸配糖体を質量分析イメージングを用いて可視化する方法についての報告です．
今週の輪読では下記の論文を取り上げました．
306. Shi, H. et al. (2013). BR-SIGNALING KINASE1 physically associates with FLAGELLIN SENSING2 and regulates plant innate immunity in Arabidopsis. Plant Cell 25: 1143–1157.
307. Paddon, C.J. et al. (2013). High-level semi-synthetic production of the potent antimalarial artemisinin. Nature 496: 528–532.
308. Toyomasu, T. et al. Reverse genetic approach to verify physiological roles of rice phytoalexins: characterization of a knockdown mutant of OsCPS4 phytoalexin biosynthetic gene in rice. Physiol. Plant. in press.
309. Saito, K. Phytochemical genomics – a new trend. Curr. Opin. Plant Biol. in press.
先週水曜日の研究室セミナーの文献紹介の担当は修士一年生の濱名君で，紹介した論文は Li, B. et al. Visualizing metabolite distribution and enzymatic conversion in plant tissues by desorption electrospray ionization mass spectrometry imaging. Plant J. in press でした．植物組織内の青酸配糖体を質量分析イメージングを用いて可視化する方法についての報告です．
今週の輪読では下記の論文を取り上げました．
306. Shi, H. et al. (2013). BR-SIGNALING KINASE1 physically associates with FLAGELLIN SENSING2 and regulates plant innate immunity in Arabidopsis. Plant Cell 25: 1143–1157.
307. Paddon, C.J. et al. (2013). High-level semi-synthetic production of the potent antimalarial artemisinin. Nature 496: 528–532.
308. Toyomasu, T. et al. Reverse genetic approach to verify physiological roles of rice phytoalexins: characterization of a knockdown mutant of OsCPS4 phytoalexin biosynthetic gene in rice. Physiol. Plant. in press.
309. Saito, K. Phytochemical genomics – a new trend. Curr. Opin. Plant Biol. in press.
2013年5月2日木曜日
今週の輪読
今週はゴールデンウイークの谷間でしたが，暦通りに輪読は 3 回行いました．今週の輪読では下記の論文を取り上げました．
303. de Wit, M. et al. Perception of low Red:Far-red ratio compromises both salicylic acid- and jasmonic acid-dependent pathogen defences in Arabidopsis. Plant J. in press.
304. Jones, A.M. et al. In vivo biochemistry: applications for small molecule biosensors in plant biology. Curr. Opin. Plant Biol. in press.
305. Su, Z.-Z. et al. (2013). Evidence for biotrophic lifestyle and biocontrol potential of dark septate endophyte Harpophora oryzae to rice blast disease. PLoS ONE 8: e61332.
303. de Wit, M. et al. Perception of low Red:Far-red ratio compromises both salicylic acid- and jasmonic acid-dependent pathogen defences in Arabidopsis. Plant J. in press.
304. Jones, A.M. et al. In vivo biochemistry: applications for small molecule biosensors in plant biology. Curr. Opin. Plant Biol. in press.
305. Su, Z.-Z. et al. (2013). Evidence for biotrophic lifestyle and biocontrol potential of dark septate endophyte Harpophora oryzae to rice blast disease. PLoS ONE 8: e61332.
2013年4月26日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
298. Solano, R., and Gimenez-Ibanez, S. (2013). Nuclear jasmonate and salicylate signaling and crosstalk in defense against pathogens. Front. Plant Sci. 4: 72.
299. Kusano, M., Iizuka, Y., Kobayashi, M., Fukushima, A., and Saito, K. (2013). Development of a direct headspace collection method from Arabidopsis seedlings using HS-SPME-GC-TOF-MS analysis. Metabolites 3: 223–242.
300. Faure, K., Bouju, E., Suchet, P., and Berthod, A. Use of limonene in countercurrent chromatography: a green alkane substitute. Anal. Chem. in press.
301. Liu, Z., Wu, J., and Huang, D. New stilbenoids isolated from fungus-challenged black skin peanut seeds and their adipogenesis inhibitory activity in 3T3-L1 cells. J. Agric. Food Chem. in press.
302. Yang, D.-L., Yang, Y., and He, Z. Role of plant hormones and their cross-talks in rice immunity. Mol. Plant in press.
298. Solano, R., and Gimenez-Ibanez, S. (2013). Nuclear jasmonate and salicylate signaling and crosstalk in defense against pathogens. Front. Plant Sci. 4: 72.
299. Kusano, M., Iizuka, Y., Kobayashi, M., Fukushima, A., and Saito, K. (2013). Development of a direct headspace collection method from Arabidopsis seedlings using HS-SPME-GC-TOF-MS analysis. Metabolites 3: 223–242.
300. Faure, K., Bouju, E., Suchet, P., and Berthod, A. Use of limonene in countercurrent chromatography: a green alkane substitute. Anal. Chem. in press.
301. Liu, Z., Wu, J., and Huang, D. New stilbenoids isolated from fungus-challenged black skin peanut seeds and their adipogenesis inhibitory activity in 3T3-L1 cells. J. Agric. Food Chem. in press.
302. Yang, D.-L., Yang, Y., and He, Z. Role of plant hormones and their cross-talks in rice immunity. Mol. Plant in press.
2013年4月24日水曜日
研究室セミナー
今週から研究室セミナーで文献紹介が始まりました．また，先週は就職活動等で発表できなかった四年生 3 名の年間計画発表も行われました．
本日の研究室セミナーの文献紹介の担当は化学生態学研究室修士二年生の吉田さんで，紹介した論文は Wright, G. A. et al. Caffeine in floral nectar enhances a pollinator’s memory of reward. Science 339, 1202-1204 (2013) でした．コーヒーノキや柑橘類の花の蜜に含まれるカフェインがミツバチの匂いの記憶を強化するという内容の論文です．
本日の研究室セミナーの文献紹介の担当は化学生態学研究室修士二年生の吉田さんで，紹介した論文は Wright, G. A. et al. Caffeine in floral nectar enhances a pollinator’s memory of reward. Science 339, 1202-1204 (2013) でした．コーヒーノキや柑橘類の花の蜜に含まれるカフェインがミツバチの匂いの記憶を強化するという内容の論文です．
2013年4月21日日曜日
年間計画発表会 & 先週の輪読
先週の水曜日には生物制御化学研究室と化学生態学研究室合同での年間計画発表会が開催されました．今年度は両研究室合計で 10 名の学生さんがいるのですが，昨年のメンバーで残っているのは 2 名だけで 8 名が新しいメンバーとなりましたので，皆まだ研究が始まったばかりです．研究は大抵の場合は計画通りには進まないものですが，うまくいかないところをうまくいくようにしたり，予想外の結果から面白い成果が得られたりするのが研究なので，10 ヶ月後の修論・卒論発表会では良い成果が発表されることを期待しています．
先週の輪読では下記の論文を取り上げました．
293. Bartley, L.E. et al. (2013). Overexpression of a BAHD acyltransferase, OsAt10, alters rice cell wall hydroxycinnamic acid content and saccharification. Plant Physiol. 161: 1615–1633.
294. Buxdorf, K., Rahat, I., Gafni, A., and Levy, M. (2013). The epiphytic fungus Pseudozyma aphidis induces jasmonic acid- and salicylic acid/Nonexpressor of PR1-independent local and systemic resistance. Plant Physiol. 161: 2014–2022.
295. Grebner, W., Stingl, N.E., Oenel, A., Mueller, M.J., and Berger, S. (2013). Lipoxygenase6-dependent oxylipin synthesis in roots is required for abiotic and biotic stress resistance of Arabidopsis. Plant Physiol. 161: 2159–2170.
296. Mou, S. et al. (2013). Functional analysis and expressional characterization of rice ankyrin repeat-containing protein, OsPIANK1, in basal defense against Magnaporthe oryzae attack. PLoS ONE 8: e59699.
297. Nobusawa, T. et al. (2013). Synthesis of very-long-chain fatty acids in the epidermis controls plant organ growth by restricting cell proliferation. PLoS Biology 11: e1001531.
先週の輪読では下記の論文を取り上げました．
293. Bartley, L.E. et al. (2013). Overexpression of a BAHD acyltransferase, OsAt10, alters rice cell wall hydroxycinnamic acid content and saccharification. Plant Physiol. 161: 1615–1633.
294. Buxdorf, K., Rahat, I., Gafni, A., and Levy, M. (2013). The epiphytic fungus Pseudozyma aphidis induces jasmonic acid- and salicylic acid/Nonexpressor of PR1-independent local and systemic resistance. Plant Physiol. 161: 2014–2022.
295. Grebner, W., Stingl, N.E., Oenel, A., Mueller, M.J., and Berger, S. (2013). Lipoxygenase6-dependent oxylipin synthesis in roots is required for abiotic and biotic stress resistance of Arabidopsis. Plant Physiol. 161: 2159–2170.
296. Mou, S. et al. (2013). Functional analysis and expressional characterization of rice ankyrin repeat-containing protein, OsPIANK1, in basal defense against Magnaporthe oryzae attack. PLoS ONE 8: e59699.
297. Nobusawa, T. et al. (2013). Synthesis of very-long-chain fatty acids in the epidermis controls plant organ growth by restricting cell proliferation. PLoS Biology 11: e1001531.
2013年4月12日金曜日
授業開始 & 今週の輪読
今週の水曜日から大学は授業が始まりました．長谷川が水戸で担当している化学生態学入門も今日が初日の講義でした．
今週の輪読では下記の論文を取り上げました．
288. Kettles, G.J., Drurey, C., Schoonbeek, H., Maule, A.J., and Hogenhout, S.A. Resistance of Arabidopsis thaliana to the green peach aphid, Myzus persicae, involves camalexin and is regulated by microRNAs. New Phytol. in press.
289. Chalfoun, N.R. et al. Purification and characterization of AsES: a subtilisin secreted by Acremonium strictum is a novel plant defense elicitor. J. Biol. Chem. in press.
290. Li, B. et al. Visualizing metabolite distribution and enzymatic conversion in plant tissues by desorption electrospray ionization mass spectrometry imaging. Plant J. in press.
291. Inokuma, Y. et al. (2013). X-ray analysis on the nanogram to microgram scale using porous complexes. Nature 495: 461–466.
292. Mugford, S.T. et al. Modularity of plant metabolic gene clusters: a trio of linked genes that are collectively required for acylation of triterpenes in oat. Plant Cell in press.
今週の輪読では下記の論文を取り上げました．
288. Kettles, G.J., Drurey, C., Schoonbeek, H., Maule, A.J., and Hogenhout, S.A. Resistance of Arabidopsis thaliana to the green peach aphid, Myzus persicae, involves camalexin and is regulated by microRNAs. New Phytol. in press.
289. Chalfoun, N.R. et al. Purification and characterization of AsES: a subtilisin secreted by Acremonium strictum is a novel plant defense elicitor. J. Biol. Chem. in press.
290. Li, B. et al. Visualizing metabolite distribution and enzymatic conversion in plant tissues by desorption electrospray ionization mass spectrometry imaging. Plant J. in press.
291. Inokuma, Y. et al. (2013). X-ray analysis on the nanogram to microgram scale using porous complexes. Nature 495: 461–466.
292. Mugford, S.T. et al. Modularity of plant metabolic gene clusters: a trio of linked genes that are collectively required for acylation of triterpenes in oat. Plant Cell in press.
2013年4月8日月曜日
井上靖乃さんの論文が Biosci. Biotechnol. Biochem. 誌に掲載
先月，修士課程を修了した井上靖乃さんの論文がBiosci. Biotechnol. Biochem. 誌にオンライン公開されました．
Inoue, Y., Sakai, M., Yao, Q., Tanimoto, Y., Toshima, H. and Hasegawa, M. (2013) Identification of a novel casbane-type diterpene phytoalexin, ent-10-oxodepressin, from rice leaves. Biosci. Biotechnol. Biochem. in press.
紫外線照射イネ葉から，新規ジテルペン化合物 ent-10-oxodepressin を単離・同定し，この化合物がイネいもち病菌接種によっても蓄積が誘導され，いもち病菌胞子に対する抗菌活性を有することも示して，イネの新規フィトアレキシンであることを明らかにした論文です．この化合物は従来知られていたイネのジテルペン系フィトアレキシンとは全く異なるカスバン骨格を持つ化合物でした．カスバン型ジテルペンとしては，トウダイグサ科植物と Sinularia 属軟質サンゴ以外の生物からは初めての発見です．また，イネのフィトアレキシンとしては 1997 年のファイトカサン E の発見の報告依頼 16 年ぶり 16 個目の発見となりました．
この研究は井上さんの修士論文研究の一部ですが，卒業・修了生の阪井美紀さん，姚群さん，谷本洋輔君の卒業論文・修士論文研究が引き継がれてまとめられたものです．
Inoue, Y., Sakai, M., Yao, Q., Tanimoto, Y., Toshima, H. and Hasegawa, M. (2013) Identification of a novel casbane-type diterpene phytoalexin, ent-10-oxodepressin, from rice leaves. Biosci. Biotechnol. Biochem. in press.
紫外線照射イネ葉から，新規ジテルペン化合物 ent-10-oxodepressin を単離・同定し，この化合物がイネいもち病菌接種によっても蓄積が誘導され，いもち病菌胞子に対する抗菌活性を有することも示して，イネの新規フィトアレキシンであることを明らかにした論文です．この化合物は従来知られていたイネのジテルペン系フィトアレキシンとは全く異なるカスバン骨格を持つ化合物でした．カスバン型ジテルペンとしては，トウダイグサ科植物と Sinularia 属軟質サンゴ以外の生物からは初めての発見です．また，イネのフィトアレキシンとしては 1997 年のファイトカサン E の発見の報告依頼 16 年ぶり 16 個目の発見となりました．
この研究は井上さんの修士論文研究の一部ですが，卒業・修了生の阪井美紀さん，姚群さん，谷本洋輔君の卒業論文・修士論文研究が引き継がれてまとめられたものです．
ent-10-oxodepressin
2013年4月5日金曜日
イネのフィトアレキシンのページ開設 & 今週の輪読
研究室ウェブサイトに新たなコンテンツとして「イネのフィトアレキシン」というページを作りました．イネフィトアレキシンに関する色々な情報を掲載していこうと考えていますが，まずは関連する文献リストを最初のコンテンツとして公開しました．このリストもまだ不完全ですが，順次アップデートしていく予定です．
新年度の新しいメンバーで今週から輪読がスタートしました．今週の輪読では下記の論文を取り上げました．
283. Zheng, Z. et al. (2013). Coordination of auxin and ethylene biosynthesis by the aminotransferase VAS1. Nat. Chem. Biol. 9: 244–246.
284. Quandt, E.M. et al. Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth. Biol. in press.
285. Vaughan, M.M. et al. Formation of the unusual semivolatile diterpene rhizathalene by the Arabidopsis class I terpene synthase TPS08 in the root stele is involved in defense against belowground herbivory. Plant Cell in press.
286. Ejike, C.E.C.C., Gong, M., and Udenigwe, C.C. Phytoalexins from the Poaceae: Biosynthesis, function and prospects in food preservation. Food Res. Int. in press.
287. Mutka, A.M., Fawley, S., Tsao, T., and Kunkel, B.N. Auxin promotes susceptibility to Pseudomonas syringae via a mechanism independent of suppression of salicylic acid-mediated defenses. Plant J. in press.
新年度の新しいメンバーで今週から輪読がスタートしました．今週の輪読では下記の論文を取り上げました．
283. Zheng, Z. et al. (2013). Coordination of auxin and ethylene biosynthesis by the aminotransferase VAS1. Nat. Chem. Biol. 9: 244–246.
284. Quandt, E.M. et al. Decaffeination and measurement of caffeine content by addicted Escherichia coli with a refactored N-demethylation operon from Pseudomonas putida CBB5. ACS Synth. Biol. in press.
285. Vaughan, M.M. et al. Formation of the unusual semivolatile diterpene rhizathalene by the Arabidopsis class I terpene synthase TPS08 in the root stele is involved in defense against belowground herbivory. Plant Cell in press.
286. Ejike, C.E.C.C., Gong, M., and Udenigwe, C.C. Phytoalexins from the Poaceae: Biosynthesis, function and prospects in food preservation. Food Res. Int. in press.
287. Mutka, A.M., Fawley, S., Tsao, T., and Kunkel, B.N. Auxin promotes susceptibility to Pseudomonas syringae via a mechanism independent of suppression of salicylic acid-mediated defenses. Plant J. in press.
2013年4月1日月曜日
新年度開始
今日から新しい年度のスタートです．今年度は新たに博士課程の学生さんも研究室に加わることになり，博士 1，修士，1，学部 4 の計 6 名の研究室となりました．修士の学生さんを除いて，6 名中 5 名が新人で，かなりガラッと顔ぶれが変わることになりました．
まだ，全員が揃ってはいませんが，今日は年度始め恒例の試薬の棚卸し作業をやってもらいました．あとちょっとで終了です．
まだ，全員が揃ってはいませんが，今日は年度始め恒例の試薬の棚卸し作業をやってもらいました．あとちょっとで終了です．
2013年3月29日金曜日
卒業式 & 日本農芸化学会 2013 年度大会
3/26（火）に茨城県武道館で茨城大学の卒業式が挙行されました．今年度は生物制御化学研究室からは修士 2 名，学士 2 名の学生さんたちが卒業することになりました．卒業論文や修士論文研究に取り組んだ過程で培った（はずの）力を生かして，社会へ出ても活躍されることを期待しています．
3/24-27 には仙台の東北大学で日本農芸化学会 2013 年度大会が開催され，研究室からは戸嶋先生と修士 2 年の大山君と私が参加しました．大山君は 3/26 に「抗β酸化型 CP-KODA (3-oxo-CP-KODA) の合成研究」というタイトルで発表を行いました．大山君は，この日は卒業式だったのですが，卒業式には参加できずにしかも引っ越し準備などで超多忙のため仙台日帰りという厳しい日程での発表となりましたが，立派に発表できたのではなかろうかと思います（私は別の会場にいたので推測）．
本日で今年度は終了です．来週からは研究室メンバーも大幅に入れ替わって新年度がスタートします．来年の明治大学での農芸化学会ではもっと多くの発表ができるようにしたいと思います．
3/24-27 には仙台の東北大学で日本農芸化学会 2013 年度大会が開催され，研究室からは戸嶋先生と修士 2 年の大山君と私が参加しました．大山君は 3/26 に「抗β酸化型 CP-KODA (3-oxo-CP-KODA) の合成研究」というタイトルで発表を行いました．大山君は，この日は卒業式だったのですが，卒業式には参加できずにしかも引っ越し準備などで超多忙のため仙台日帰りという厳しい日程での発表となりましたが，立派に発表できたのではなかろうかと思います（私は別の会場にいたので推測）．
本日で今年度は終了です．来週からは研究室メンバーも大幅に入れ替わって新年度がスタートします．来年の明治大学での農芸化学会ではもっと多くの発表ができるようにしたいと思います．
2013年3月22日金曜日
謝恩会 & 今週の輪読
今年の茨城大学卒業式は 3/26（火）に開催予定なのですが，資源生物科学科の多くの教員と学生が参加する日本農芸化学会（3/24-27）と日程が重なってしまったため，謝恩会は 3/18（月）につくばのホテルグランド東雲で開催されました．今年はうちの研究室の学生さんたちが当番で幹事になっていたので，準備や司会で大変だったようですが，無事に楽しい謝恩会が開催できたと思います．
今週の輪読では下記の論文を取り上げました．来週は輪読はお休みで，再来週から新年度の体制で再開予定です．
279. Wang, Y. et al. The Arabidopsis Elongator complex subunit2 epigenetically regulates plant immune responses. Plant Cell in press.
280. Li, W. et al. Oscyp71Z2 involves diterpenoid phytoalexin biosynthesis that contributes to bacterial blight resistance in rice. Plant Sci. in press.
281. Wright, G.A. et al. (2013). Caffeine in floral nectar enhances a pollinator’s memory of reward. Science 339: 1202–1204.
282. Yamaguchi, K. et al. (2013). A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Cell Host Microbe 13: 347–357.
今週の輪読では下記の論文を取り上げました．来週は輪読はお休みで，再来週から新年度の体制で再開予定です．
279. Wang, Y. et al. The Arabidopsis Elongator complex subunit2 epigenetically regulates plant immune responses. Plant Cell in press.
280. Li, W. et al. Oscyp71Z2 involves diterpenoid phytoalexin biosynthesis that contributes to bacterial blight resistance in rice. Plant Sci. in press.
281. Wright, G.A. et al. (2013). Caffeine in floral nectar enhances a pollinator’s memory of reward. Science 339: 1202–1204.
282. Yamaguchi, K. et al. (2013). A receptor-like cytoplasmic kinase targeted by a plant pathogen effector is directly phosphorylated by the chitin receptor and mediates rice immunity. Cell Host Microbe 13: 347–357.
2013年3月15日金曜日
新しい遠心エバポレーター & 今週の輪読
試料溶液の濃縮操作ではロータリーエバポレーターが多用されますが，少量試料を同時に多数濃縮する必要がある場合には遠心エバポレーターが便利です．研究室では 15 年以上使っている東京理化の遠心エバポレーターがあったのですが，やや回転が不安定で昨年は一回壊れて修理に出したりしていました．幸い予算の都合が付いたので，東京理化の遠心エバポレーター CVE-3100 を新たに購入することができました．同時に同じく 15 年以上使っていたバキューブランドのダイヤフラム真空ポンプ（スイッチが壊れていて，ときどき電源オンできなくなっていた）もビュッヒの V-700 に置き換えることにしました．今週から使い始めましたが，新しい遠心エバポレーターは回転音が非常に回転音が静かです．
今週の輪読では下記の論文を取り上げました．
274. Carvalhais, L.C. et al. (2013). Activation of the jasmonic acid plant defence pathway alters the composition of rhizosphere bacterial communities. PLoS ONE 8: e56457.
275. Simmler, C. et al. (2013). Dynamic residual complexity of the isoliquiritigenin–liquiritigenin interconversion during bioassay. J. Agric. Food Chem. 61: 2146–2157.
276. Van der Does, D. et al. Salicylic acid suppresses jasmonic acid signaling downstream of SCFCOI1-JAZ by targeting GCC promoter motifs via transcription factor ORA59. Plant Cell in press.
277. Datta, S., Zhou, Y.-D., and Nagle, D.G. Comparative study of chromatographic medium-associated mass and potential antitumor activity loss with bioactive extracts. J. Nat. Prod. in press.
278. Shah, J., and Zeier, J. (2013). Long-distance communication and signal amplification in systemic acquired resistance. Front. Plant Sci. 4: 30.
新しい遠心エバポレーター．左のウォーターバスの奥にあるのが新しい真空ポンプ
274. Carvalhais, L.C. et al. (2013). Activation of the jasmonic acid plant defence pathway alters the composition of rhizosphere bacterial communities. PLoS ONE 8: e56457.
275. Simmler, C. et al. (2013). Dynamic residual complexity of the isoliquiritigenin–liquiritigenin interconversion during bioassay. J. Agric. Food Chem. 61: 2146–2157.
276. Van der Does, D. et al. Salicylic acid suppresses jasmonic acid signaling downstream of SCFCOI1-JAZ by targeting GCC promoter motifs via transcription factor ORA59. Plant Cell in press.
277. Datta, S., Zhou, Y.-D., and Nagle, D.G. Comparative study of chromatographic medium-associated mass and potential antitumor activity loss with bioactive extracts. J. Nat. Prod. in press.
278. Shah, J., and Zeier, J. (2013). Long-distance communication and signal amplification in systemic acquired resistance. Front. Plant Sci. 4: 30.
2013年3月8日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
269. Del Terra, L. et al. Functional characterization of three Coffea arabica L. monoterpene synthases: Insights into the enzymatic machinery of coffee aroma. Phytochemistry in press.
270. Sobolev, V.S. (2013). Production of phytoalexins in peanut (Arachis hypogaea) seed elicited by selected microorganisms. J. Agric. Food Chem. 61: 1850–1858.
271. Hentrich, M. et al. The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression. Plant J. in press.
272. Clarke, D., Whitney, H., Sutton, G., and Robert, D. Detection and learning of floral electric fields by bumblebees. Science in press.
273. Mazzeo, G. et al. Absolute configurations of fungal and plant metabolites by chiroptical methods. ORD, ECD, and VCD studies on phyllostin, scytolide, and oxysporone. J. Nat. Prod. in press.
269. Del Terra, L. et al. Functional characterization of three Coffea arabica L. monoterpene synthases: Insights into the enzymatic machinery of coffee aroma. Phytochemistry in press.
270. Sobolev, V.S. (2013). Production of phytoalexins in peanut (Arachis hypogaea) seed elicited by selected microorganisms. J. Agric. Food Chem. 61: 1850–1858.
271. Hentrich, M. et al. The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression. Plant J. in press.
272. Clarke, D., Whitney, H., Sutton, G., and Robert, D. Detection and learning of floral electric fields by bumblebees. Science in press.
273. Mazzeo, G. et al. Absolute configurations of fungal and plant metabolites by chiroptical methods. ORD, ECD, and VCD studies on phyllostin, scytolide, and oxysporone. J. Nat. Prod. in press.
2013年3月4日月曜日
先週の輪読
先週の輪読では下記の論文を取り上げました．先週の月曜日は入学試験だったので，学生の構内立ち入りが制限されていたため，輪読は中止しました．
265. Yan, J. et al. The Arabidopsis F-Box protein CORONATINE INSENSITIVE1 is stabilized by SCFCOI1 and degraded via the 26S proteasome pathway. Plant Cell in press.
266. Cheng, X., Zhu, L., and He, G. The understanding of molecular interaction between rice and brown planthopper. Mol. Plant in press.
267. Neilson, E.H., Goodger, J.Q.D., Woodrow, I.E., and Møller, B.L. Plant chemical defense: at what cost? Trends Plant Sci. in press.
268. Cecchetti, V. et al. Auxin controls Arabidopsis anther dehiscence by regulating endothecium lignification and jasmonic acid biosynthesis. Plant J. in press.
265. Yan, J. et al. The Arabidopsis F-Box protein CORONATINE INSENSITIVE1 is stabilized by SCFCOI1 and degraded via the 26S proteasome pathway. Plant Cell in press.
266. Cheng, X., Zhu, L., and He, G. The understanding of molecular interaction between rice and brown planthopper. Mol. Plant in press.
267. Neilson, E.H., Goodger, J.Q.D., Woodrow, I.E., and Møller, B.L. Plant chemical defense: at what cost? Trends Plant Sci. in press.
268. Cecchetti, V. et al. Auxin controls Arabidopsis anther dehiscence by regulating endothecium lignification and jasmonic acid biosynthesis. Plant J. in press.
2013年2月28日木曜日
新しい真空ポンプ
今日は新しいビュッヒのダイヤフラム真空ポンプの設置作業がありました．研究室では有機溶媒中に溶けている化合物を取り出すために，頻繁に濃縮操作が行われます．そのためにはロータリーエバポレーターや遠心エバポレーターという機器が用いられるのですが，これらは大気圧よりも圧力を低くすることによって液体の沸点を低下させて効率よく溶媒を蒸発させるものです．これらの機器を用いるためには真空ポンプが必要となるのですが，真空ポンプで減圧するときに圧力を低くしすぎると突沸という急激に沸騰が起こる状態になり，困ったことになります．従来は非常に難しいコックの操作をして突沸が起こらないように対応していたのですが，新しい真空ポンプは圧力を精密に制御できるコントローラーが付いているので，エバポレーター使用中の突沸がかなり防げるのではないかと期待しています．
新しいビュッヒの真空ポンプ V-700 + コントローラー V-850
2013年2月22日金曜日
超音波洗浄器更新
研究室で私が学生だった頃から二十年以上使っている超音波洗浄器（シャープ製）があるのですが，一昨年ぐらいからタイマーが壊れてしまいました．超音波自体には特に問題がなかったため，そのまま使い続けていたのですが，予算の都合が付いたので，新しい超音波洗浄器を購入しました．アズワンで売っているものを購入したのですが，従来のものに比べると洗浄槽が浅いのが難点です．また，超音波の出力もだいぶ弱いような気がします．安価なものを買ったので，仕方がないところなのかもしれません．古い方の洗浄器もまだ使えないわけではないので，サブとしてしばらくは使い続ける予定です．
新しい超音波洗浄器
20 年以上使っている超音波洗浄器．さびてぼろぼろだけれど，タイマー以外は問題なし．
今週の輪読
今週の輪読では下記の論文を取り上げました．今週から来年度研究室に配属される三年生が実験の引き継ぎのために研究室に来るようになりましたので，輪読にも参加してもらうことにしました．
260. Derewacz, D.K., Goodwin, C.R., McNees, C.R., McLean, J.A., and Bachmann, B.O. (2013). Antimicrobial drug resistance affects broad changes in metabolomic phenotype in addition to secondary metabolism. Proc. Natl. Acad. Sci. USA 110: 2336–2341.
261. Fuchs, J. et al. (2013). A noninvasive platform for imaging and quantifying oil storage in submillimeter tobacco seed. Plant Physiol. 161: 583–593.
262. Hall, D.E. et al. (2013). Evolution of conifer diterpene synthases: diterpene resin acid biosynthesis in lodgepole pine and jack pine involves monofunctional and bifunctional diterpene synthases. Plant Physiol. 161: 600–616.
263. Kollner, T. et al. (2013). Localization of sesquiterpene formation and emission in maize leaves after herbivore damage. BMC Plant Biol. 13: 15.
264. Wahl, V. et al. (2013). Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana. Science 339: 704–707.
260. Derewacz, D.K., Goodwin, C.R., McNees, C.R., McLean, J.A., and Bachmann, B.O. (2013). Antimicrobial drug resistance affects broad changes in metabolomic phenotype in addition to secondary metabolism. Proc. Natl. Acad. Sci. USA 110: 2336–2341.
261. Fuchs, J. et al. (2013). A noninvasive platform for imaging and quantifying oil storage in submillimeter tobacco seed. Plant Physiol. 161: 583–593.
262. Hall, D.E. et al. (2013). Evolution of conifer diterpene synthases: diterpene resin acid biosynthesis in lodgepole pine and jack pine involves monofunctional and bifunctional diterpene synthases. Plant Physiol. 161: 600–616.
263. Kollner, T. et al. (2013). Localization of sesquiterpene formation and emission in maize leaves after herbivore damage. BMC Plant Biol. 13: 15.
264. Wahl, V. et al. (2013). Regulation of flowering by trehalose-6-phosphate signaling in Arabidopsis thaliana. Science 339: 704–707.
2013年2月15日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
256. Nawkar, G.M. et al. (2013). UV-induced cell death in plants. Int. J. Mol. Sci. 14: 1608–1628.
257. Kato-Noguchi, H., Ota, K., Kujime, H., and Ogawa, M. Effects of momilactone on the protein expression in Arabidopsis germination. Weed Biol. Manage. in press.
258. Pourcel, L. et al. A chemical complementation approach reveals genes and interactions of flavonoids with other pathways. Plant J. in press.
259. Li, W., Lin, H., Flarakos, J., and Tse, F.L.S. Practical approaches to incurred sample LC-MS/MS reanalysis: confirming unexpected results. Anal. Chem. in press.
256. Nawkar, G.M. et al. (2013). UV-induced cell death in plants. Int. J. Mol. Sci. 14: 1608–1628.
257. Kato-Noguchi, H., Ota, K., Kujime, H., and Ogawa, M. Effects of momilactone on the protein expression in Arabidopsis germination. Weed Biol. Manage. in press.
258. Pourcel, L. et al. A chemical complementation approach reveals genes and interactions of flavonoids with other pathways. Plant J. in press.
259. Li, W., Lin, H., Flarakos, J., and Tse, F.L.S. Practical approaches to incurred sample LC-MS/MS reanalysis: confirming unexpected results. Anal. Chem. in press.
2013年2月14日木曜日
卒業論文発表会
今日と明日は資源生物科学科の卒業論文発表会が開催されています．生物制御化学研究室の四年生 2 名は今日の午後の順番だったので，すでに無事に発表を終えました．平石由貴さんが「イネフィトアレキシン モミラクトンAのいもち病菌による代謝」，渡邊峰花さんが「カビ毒ニバレノールの簡易スクリーニング手法の開発 ―ニバレノール抗原および蛍光トレーサーの合成研究―」というタイトルでの発表でした．二人とも発表自体はまあまあの出来だったと思いますが，どちらも研究内容が目標到達まであと一歩（数歩？）というところで時間切れになってしまったのが残念なところでした．卒論発表会は他の研究室の学生さんの発表が明日も続きます．また，明日は卒業論文の提出〆切日にもなっていますので，もうひと頑張りが必要です．
2013年2月12日火曜日
修士論文発表会
今日は茨城大学大学院農学研究科資源生物科学専攻の修士論文発表会が開催されました．農業化学生態学専門分野の発表は午前中で，生物制御化学研究室からは昼休み前に井上靖乃さんと大山秀芳君の発表がそれぞれ「紫外線照射イネ葉に蓄積するストレス誘導性化合物に関する研究」と「花芽誘導活性を有する脂肪酸誘導体の合成研究」というタイトルでありました．二人とも落ち着いて良い発表ができたと思います．この後は木曜日に研究室の卒論学生 2 名の卒業論文発表会が予定されていて，現在準備がラストスパートに入っているところです．
2013年2月8日金曜日
修論・卒論，期末試験，今週の輪読
いよいよ修士論文や卒業論文が大詰めの時期になってきました．先週末の 2/1 が修士論文の提出〆切日で生物制御化学研究室の学生さんたちの修士論文予備審査会は今週の水曜日 2/6 の午後に開催されました．2/7 には修士論文と卒業論文の発表練習会が化学生態学研究室と生物制御化学研究室合同で開催され，来週の本番の発表に向けて多くの要改善点が指摘されました．修士論文発表会は来週火曜日，卒業論文発表会は木，金曜日に開催予定です．また，今日で期末試験が終わりましたので，研究室に所属していない学生さんは明日から二ヶ月間の長い春休みに入ります．四年生と修士二年生の学生はもうひと頑張りです．
今週の輪読では下記の論文を取り上げました．
251. Huigens, R.W. et al. A ring-distortion strategy to construct stereochemically complex and structurally diverse compounds from natural products. Nat. Chem. in press.
252. Mornar, A., Sertić, M., and Nigović, B. (2013). Development of a rapid LC/DAD/FLD/MSn method for the simultaneous determination of monacolins and citrinin in red fermented rice products. J. Agric. Food Chem. 61: 1072–1080.
253. Riemann, M. et al. Identification of rice ALLENE OXIDE CYCLASE mutants and the function of jasmonate for defence against Magnaporthe oryzae. Plant J. in press.
254. Nwachukwu, I.D., Luciano, F.B., and Udenigwe, C.C. The inducible soybean glyceollin phytoalexins with multifunctional health-promoting properties. Food Res. Int. in press.
255. Avizcuri-Inac, J.-M. et al. (2013). Effect of cluster thinning and prohexadione calcium applications on phenolic composition and sensory properties of red wines. J. Agric. Food Chem. 61: 1124–1137.
今週の輪読では下記の論文を取り上げました．
251. Huigens, R.W. et al. A ring-distortion strategy to construct stereochemically complex and structurally diverse compounds from natural products. Nat. Chem. in press.
252. Mornar, A., Sertić, M., and Nigović, B. (2013). Development of a rapid LC/DAD/FLD/MSn method for the simultaneous determination of monacolins and citrinin in red fermented rice products. J. Agric. Food Chem. 61: 1072–1080.
253. Riemann, M. et al. Identification of rice ALLENE OXIDE CYCLASE mutants and the function of jasmonate for defence against Magnaporthe oryzae. Plant J. in press.
254. Nwachukwu, I.D., Luciano, F.B., and Udenigwe, C.C. The inducible soybean glyceollin phytoalexins with multifunctional health-promoting properties. Food Res. Int. in press.
255. Avizcuri-Inac, J.-M. et al. (2013). Effect of cluster thinning and prohexadione calcium applications on phenolic composition and sensory properties of red wines. J. Agric. Food Chem. 61: 1124–1137.
2013年2月1日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
246. Wells, D.M., Laplaze, L., Bennett, M.J., and Vernoux, T. Biosensors for phytohormone quantification: challenges, solutions, and opportunities. Trends Plant Sci. in press.
247. Balmer, D., de Papajewski, D.V., Planchamp, C., Glauser, G., and Mauch-Mani, B. Induced resistance in maize is based on organ-specific defence responses. Plant J. in press.
248. Tagami, K. et al. (2013). Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae. J. Am. Chem. Soc. 135: 1260–1263.
249. Banasiak, J. et al. A Medicago truncatula ABC transporter belonging to subfamily G modulates the level of isoflavonoids. J. Exp. Bot. in press.
250. Lee, H.Y. et al. (2013). Oryza sativa COI homologues restore jasmonate signal transduction in Arabidopsis coi1-1 mutants. PLoS ONE 8: e52802.
246. Wells, D.M., Laplaze, L., Bennett, M.J., and Vernoux, T. Biosensors for phytohormone quantification: challenges, solutions, and opportunities. Trends Plant Sci. in press.
247. Balmer, D., de Papajewski, D.V., Planchamp, C., Glauser, G., and Mauch-Mani, B. Induced resistance in maize is based on organ-specific defence responses. Plant J. in press.
248. Tagami, K. et al. (2013). Reconstitution of biosynthetic machinery for indole-diterpene paxilline in Aspergillus oryzae. J. Am. Chem. Soc. 135: 1260–1263.
249. Banasiak, J. et al. A Medicago truncatula ABC transporter belonging to subfamily G modulates the level of isoflavonoids. J. Exp. Bot. in press.
250. Lee, H.Y. et al. (2013). Oryza sativa COI homologues restore jasmonate signal transduction in Arabidopsis coi1-1 mutants. PLoS ONE 8: e52802.
2013年1月25日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
241. Renata, H., Zhou, Q., and Baran, P.S. (2013). Strategic redox relay enables a scalable synthesis of ouabagenin, a bioactive cardenolide. Science 339: 59–63.
242. Ding, S.-Y. et al. (2012). How does plant cell wall nanoscale architecture correlate with enzymatic digestibility? Science 338: 1055–1060.
243. Cueto, D., Sergio, Encinar, J.R., Sanz-Medel, A., and Alonso, J.I.G. Liquid chromatography, chemical oxidation, and online carbon isotope dilution mass spectrometry as a universal quantification system for nonvolatile organic compounds. Anal. Chem. in press.
244. Quadri, S.S., Stratford, R.E., Boué, S.M., and Cole, R.B. Screening and identification of glyceollins and their metabolites by electrospray ionization tandem mass spectrometry with precursor ion scanning. Anal. Chem. in press.
245. Sharma, R., Vleesschauwer, D.D., Sharma, M.K., and Ronald, P.C. Elucidating stress regulatory crosstalk in rice. Mol. Plant in press.
241. Renata, H., Zhou, Q., and Baran, P.S. (2013). Strategic redox relay enables a scalable synthesis of ouabagenin, a bioactive cardenolide. Science 339: 59–63.
242. Ding, S.-Y. et al. (2012). How does plant cell wall nanoscale architecture correlate with enzymatic digestibility? Science 338: 1055–1060.
243. Cueto, D., Sergio, Encinar, J.R., Sanz-Medel, A., and Alonso, J.I.G. Liquid chromatography, chemical oxidation, and online carbon isotope dilution mass spectrometry as a universal quantification system for nonvolatile organic compounds. Anal. Chem. in press.
244. Quadri, S.S., Stratford, R.E., Boué, S.M., and Cole, R.B. Screening and identification of glyceollins and their metabolites by electrospray ionization tandem mass spectrometry with precursor ion scanning. Anal. Chem. in press.
245. Sharma, R., Vleesschauwer, D.D., Sharma, M.K., and Ronald, P.C. Elucidating stress regulatory crosstalk in rice. Mol. Plant in press.
2013年1月18日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
237. Chamam, A. et al. Plant secondary metabolite profiling evidences strain-dependent effect in the Azospirillum–Oryza sativa association. Phytochemistry in press.
238. Coelho, P.S., Brustad, E.M., Kannan, A., and Arnold, F.H. (2013). Olefin cyclopropanation via carbene transfer catalyzed by engineered cytochrome P450 enzymes. Science 339: 307–310.
239. Kim, T.-H. et al. Natural variation in small molecule–induced TIR-NB-LRR signaling induces root growth arrest via EDS1- and PAD4-complexed R protein VICTR in Arabidopsis. Plant Cell in press.
240. Sasaki, E. et al. Uniconazole, a cytochrome P450 inhibitor, inhibits trans-zeatin biosynthesis in Arabidopsis. Phytochemistry in press.
237. Chamam, A. et al. Plant secondary metabolite profiling evidences strain-dependent effect in the Azospirillum–Oryza sativa association. Phytochemistry in press.
238. Coelho, P.S., Brustad, E.M., Kannan, A., and Arnold, F.H. (2013). Olefin cyclopropanation via carbene transfer catalyzed by engineered cytochrome P450 enzymes. Science 339: 307–310.
239. Kim, T.-H. et al. Natural variation in small molecule–induced TIR-NB-LRR signaling induces root growth arrest via EDS1- and PAD4-complexed R protein VICTR in Arabidopsis. Plant Cell in press.
240. Sasaki, E. et al. Uniconazole, a cytochrome P450 inhibitor, inhibits trans-zeatin biosynthesis in Arabidopsis. Phytochemistry in press.
2013年1月11日金曜日
今週の輪読
今週の輪読では下記の論文を取り上げました．
232. Rawat, N., Himabindu, K., Neeraja, C.N., Nair, S., and Bentur, J.S. (2013). Suppressive subtraction hybridization reveals that rice gall midge attack elicits plant-pathogen-like responses in rice. Plant Physiol. Biochem. 63: 122–130.
233. Bagnaresi, P. et al. (2012). Comparative transcriptome profiling of the early response to Magnaporthe oryzae in durable resistant vs susceptible rice (Oryza sativa L.) genotypes. PLoS ONE 7: e51609.
234. Ballhorn, D., Kautz, S., and Schädler, M. Induced plant defense via volatile production is dependent on rhizobial symbiosis. Oecologia in press.
235. Ribot, C. et al. The Magnaporthe oryzae effector AVR1-C039 is translocated into rice cells independently of a fungal-derived machinery. Plant J. in press.
236. Desaki, Y. et al. (2012). Positive crosstalk of MAMP signaling pathways in rice cells. PLoS ONE 7: e51953.
232. Rawat, N., Himabindu, K., Neeraja, C.N., Nair, S., and Bentur, J.S. (2013). Suppressive subtraction hybridization reveals that rice gall midge attack elicits plant-pathogen-like responses in rice. Plant Physiol. Biochem. 63: 122–130.
233. Bagnaresi, P. et al. (2012). Comparative transcriptome profiling of the early response to Magnaporthe oryzae in durable resistant vs susceptible rice (Oryza sativa L.) genotypes. PLoS ONE 7: e51609.
234. Ballhorn, D., Kautz, S., and Schädler, M. Induced plant defense via volatile production is dependent on rhizobial symbiosis. Oecologia in press.
235. Ribot, C. et al. The Magnaporthe oryzae effector AVR1-C039 is translocated into rice cells independently of a fungal-derived machinery. Plant J. in press.
236. Desaki, Y. et al. (2012). Positive crosstalk of MAMP signaling pathways in rice cells. PLoS ONE 7: e51953.
2013年1月10日木曜日
研究室セミナー
本日の研究室セミナーの文献紹介の担当は化学生態学研究室四年生の貫井君で，紹介した論文は Behie SW, Zelisko PM, Bidochka MJ (2012) Endophytic insect-parasitic fungi translocate nitrogen directly from insects to plants. Science 336: 1576–1577 でした．昆虫の病原菌でもあるエンドファイトが植物へ窒素を供給しているという内容の論文です．
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